[Objectives]This study was conducted to explore the feasibility of planting pepino(Solanum muricatum Ait.)in Qinghai Province,and to understand the cultivation quality and yield in various areas of Qinghai.[Methods]Wi...[Objectives]This study was conducted to explore the feasibility of planting pepino(Solanum muricatum Ait.)in Qinghai Province,and to understand the cultivation quality and yield in various areas of Qinghai.[Methods]With small fruit type of pepino as the material,four areas in eastern Qinghai were selected to determine the agronomic traits,yield and quality indexes of pepino.[Results]Under the same cultivation conditions,there were some differences in the cultivation status of pepino,but overall,pepino fruit had higher quality.Various physiological indexes were correlated with quality and yield.[Conclusions]This study clarified the specific cultivation situation of pepino in Qinghai Province,and evaluated the quality and yield of pepino,providing strong data support for the promotion and planting of pepino in various regions in the future.展开更多
MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attra...MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attracted significant interest, as abnormal miRNA expression is identified to contribute to serious human diseases such as cancers. Traditional methods for miRNA detection do not meet current demands. In particular, nanomaterial-based methods, nucleic acid amplification-based methods such as rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), strand-displacement amplification (SDA) and some enzyme-free amplifications have been employed widely for the highly sensitive detection of miRNA. MiRNA functional research and clinical diagnostics have been accelerated by these new techniques. Herein, we summarize and discuss the recent progress in the development of miRNA detection methods and new applications. This review will provide guidelines for the development of follow-up miRNA detection methods with high sensitivity and specificity, and applicability to disease diagnosis and therapy.展开更多
基金Supported by Science and Technology Demonstration of Rural Revitalization in Jianzha County(2023-NK-X01)。
文摘[Objectives]This study was conducted to explore the feasibility of planting pepino(Solanum muricatum Ait.)in Qinghai Province,and to understand the cultivation quality and yield in various areas of Qinghai.[Methods]With small fruit type of pepino as the material,four areas in eastern Qinghai were selected to determine the agronomic traits,yield and quality indexes of pepino.[Results]Under the same cultivation conditions,there were some differences in the cultivation status of pepino,but overall,pepino fruit had higher quality.Various physiological indexes were correlated with quality and yield.[Conclusions]This study clarified the specific cultivation situation of pepino in Qinghai Province,and evaluated the quality and yield of pepino,providing strong data support for the promotion and planting of pepino in various regions in the future.
基金financial support from the National Natural Science Foundation of China(Grant 81573389)the National Key R&D Program of China(2017YFC0908600)
文摘MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attracted significant interest, as abnormal miRNA expression is identified to contribute to serious human diseases such as cancers. Traditional methods for miRNA detection do not meet current demands. In particular, nanomaterial-based methods, nucleic acid amplification-based methods such as rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), strand-displacement amplification (SDA) and some enzyme-free amplifications have been employed widely for the highly sensitive detection of miRNA. MiRNA functional research and clinical diagnostics have been accelerated by these new techniques. Herein, we summarize and discuss the recent progress in the development of miRNA detection methods and new applications. This review will provide guidelines for the development of follow-up miRNA detection methods with high sensitivity and specificity, and applicability to disease diagnosis and therapy.
