Microbial production of monoterpenoid indole alkaloids(MIAs)provides a sustainable and eco-friendly means to obtain compounds with high pharmaceutical values.However,efficient biosynthesis of MIAs in heterologous micr...Microbial production of monoterpenoid indole alkaloids(MIAs)provides a sustainable and eco-friendly means to obtain compounds with high pharmaceutical values.However,efficient biosynthesis of MIAs in heterologous microorganisms is hindered due to low supply of key precursors such as geraniol and its derivative 8-hydroxygeraniol catalyzed by geraniol 8-hydroxylase(G8H).In this study,we developed a facile evolution platform to screen strains with improved yield of geraniol by using the SCRaMbLE system embedded in the Sc2.0 synthetic yeast and confirmed the causal role of relevant genomic targets.Through genome mining,we identified several G8H enzymes that perform much better than the commonly used CrG8H for 8-hydroxygeraniol production in vivo.We further showed that the N-terminus of these G8H enzymes plays an important role in cellular activity by swapping experiments.Finally,the combination of the engineered chassis,optimized biosynthesis pathway,and utilization of G8H led to the final strain with more than 30-fold improvement in producing 8-hydroxygeraniol compared with the starting strain.Overall,this study will provide insights into the construction and optimization of yeast cells for efficient biosynthesis of 8-hydroxygeraniol and its derivatives.展开更多
Green building development in China relies mainly on compulsory measures that lack incentive policies to motivate the enthusiasm of real estate developers.A floor area ratio bonus is encouraged by the Chinese State Co...Green building development in China relies mainly on compulsory measures that lack incentive policies to motivate the enthusiasm of real estate developers.A floor area ratio bonus is encouraged by the Chinese State Council.In order to ensure the feasibility of a reward quota,residential buildings that met the requirement for energy efficiency during the official assessment in Ningbo in 2014 were selected as research objects.The amount of energy and water savings in terms of the Assessment Standard for Green Building is converted into carbon emissions.Carbon emissions of different star-rated green buildings are then measured in accordance with the actual water and power consumption of residential dwellings in 2014.A regression equation is set up concerning the floor area ratio and index for residential land per capita.A carbon emission-based method is proposed for measuring the reward quota associated with floor area ratio and recommendations are given for development using green building.展开更多
基金supported by grants from the National Key Research and Development Program of China (No.2021YFC2100800 to Y.Z.)the Science,Technology and Innovation Commission of Shenzhen Municipality under grant (No.JCYJ20180507183534578)the Tip-top Scientific and Technical Innovative Youth Talents of Guangdong Special Support Program (2019TQ05Y876 to Y.S.)。
文摘Microbial production of monoterpenoid indole alkaloids(MIAs)provides a sustainable and eco-friendly means to obtain compounds with high pharmaceutical values.However,efficient biosynthesis of MIAs in heterologous microorganisms is hindered due to low supply of key precursors such as geraniol and its derivative 8-hydroxygeraniol catalyzed by geraniol 8-hydroxylase(G8H).In this study,we developed a facile evolution platform to screen strains with improved yield of geraniol by using the SCRaMbLE system embedded in the Sc2.0 synthetic yeast and confirmed the causal role of relevant genomic targets.Through genome mining,we identified several G8H enzymes that perform much better than the commonly used CrG8H for 8-hydroxygeraniol production in vivo.We further showed that the N-terminus of these G8H enzymes plays an important role in cellular activity by swapping experiments.Finally,the combination of the engineered chassis,optimized biosynthesis pathway,and utilization of G8H led to the final strain with more than 30-fold improvement in producing 8-hydroxygeraniol compared with the starting strain.Overall,this study will provide insights into the construction and optimization of yeast cells for efficient biosynthesis of 8-hydroxygeraniol and its derivatives.
基金supported by Zhejiang Provincial Construction Research Projects(2015K150)Natural Science Foundation of Ningbo(2015A610302).
文摘Green building development in China relies mainly on compulsory measures that lack incentive policies to motivate the enthusiasm of real estate developers.A floor area ratio bonus is encouraged by the Chinese State Council.In order to ensure the feasibility of a reward quota,residential buildings that met the requirement for energy efficiency during the official assessment in Ningbo in 2014 were selected as research objects.The amount of energy and water savings in terms of the Assessment Standard for Green Building is converted into carbon emissions.Carbon emissions of different star-rated green buildings are then measured in accordance with the actual water and power consumption of residential dwellings in 2014.A regression equation is set up concerning the floor area ratio and index for residential land per capita.A carbon emission-based method is proposed for measuring the reward quota associated with floor area ratio and recommendations are given for development using green building.
