Differential expression of serum miR-126,miR-141 and miR-21 as novel biomarkers for early detection of liver metastasis in colorectal cancer

Objective：MicroRNAs （miRNAs） have potential as diagnostic biomarkers in cancer.Evaluation of the association between miRNA expression patterns and early detection of liver metastasis in colorectal cancer （CRC） has not been reported.Methods：We investigated the expression of metastasis-associated miRs-31,335,206,141,126,200b,200c,21,Let7a,Let7b and Let7c in localized,liver-metastatic and other organ-metastatic CRC （OM-CRC）.Expressions of target miRNAs in serum were evaluated in 116 consecutive localized CRC （L-CRC）,72 synchronous liver-metastatic CRC （SLM-CRC） and 36 other OM-CRC by quantitative real-time PCR.Results：Seven of 11 tested miRNAs could be detected from serum.Four miRNAs,miR-126,Let-7a,miR141 and miR-21 were identified as metastasis-associated miRNAs.Compared with L-CRC,significant upregulated expression was observed for miR-141 and miR-21 in SLM-CRC and OM-CRC,down-regulated expression was observed for miR-126 in SLM-CRC and OM-CRC,and up-regulated expression of Let-7a in OM-CRC.The receiver operating characteristic （ROC） curve showed serum miR-126 had a cut-off [log10 relative quantity （log10RQ）=--0.2005] with 77.78％ sensitivity and 68.97％ specificity with an area under curve （AUC） of 0.7564,miR-141 had a cut-off （1og10RQ=-0.2285） with 86.11％ sensitivity and 76.11％ specificity with an AUC of 0.8279,and miR-21 had a cut-off （log10RQ=-0.1310） with 73.61％ sensitivity and 66.38％ specificity with an AUC of 0.7479.Conclusions：We identified liver metastasis-associated miRNAs,suggesting serum miR-126,miR-141 and miR-21 may be novel biomarkers for clinical diagnosis of early stage liver-metastatic CRC.

氮硫硅协效阻燃剂的合成及在聚碳酸酯中的应用

为研究氮硫硅元素间的协效阻燃作用,以三聚氯氰、氨基乙磺酸、对氨基苯酚和1,3-双(3-氨基丙基)四甲基二硅氧烷为原料,通过亲核取代反应制备了一种含氮硫硅的阻燃剂2,2’-(1,1,3,3-四甲基二硅氧烷-1,3-二基)双(丙烷-3,1-二基)-双(4-氨基乙磺酸钾-6-(4-(羟基苯基)氨基)-1,3,5-均三嗪)(KTS),并用熔融共混技术制备了聚碳酸酯(PC)/KTS复合材料。采用热重分析(TGA)、极限氧指数(LOI)、垂直燃烧(UL 94)及锥形量热(CONE)研究了复合材料的热稳定性和燃烧性能。结果表明,KTS的加入虽然降低了复合材料的热稳定性,但也促使复合材料提前分解成炭,抑制了材料的进一步分解;当KTS的质量分数为0.5%时,复合材料的LOI提高到34.6%,并通过了UL 94 V-0级;复合材料的热释放峰值和烟释放峰值分别降低了41.3%和35.3%。扫描电镜和拉曼光谱结果表明,KTS的加入提高了炭层的表面规整性和石墨化程度,起到良好的凝聚相阻燃作用。

EBI2 Regulating the Migration of B cells in T-dependent Antibody Responses

EBI2 is known as a kind of 7TM G protein-coupled receptors, and EBi2 gene is one of the most upregulated genes in Epstein-Barr virus （EBV） -infected lymphocytes. As a constitutively active receptor, EBI2 signals via Gai, and TM-II plays an important role in the signal transduct/on process. EBI2 is expressed mainly in mature, B cells and the expression level increases at the early stage of activation; subsequently, B cells are transferred into GC and the expression level is down-regulated. EBI2 can regulate independently and mediate synergistically the migration of B cell towards the extrafollicular areas and GC depending on chemo- kine receptors CXCR5 and CCR7 （EBII）. Differential expression of EBI2 receptor is conducive to accurate migration of B cells at different stages, thereby leading to appropriate irranunologic responses to T-dependent antigens. Recently, two research groups identified a specific oxysteml as the natural ligand for EBL2. Among all the oxysterols, 7α, 25-Dihydrexycholesteml （7a, 25-OHC） is the most effective ligand and activator, and EBI2-Oxysterol signal pathway plays an important role in adaptive immune responses. In addition, EBI2 is also closely related with specific kinds of autoimmune diseases, so it is considered as a negative regulator of the innate immune response in macrophages.

Application of M13 Phage Coat Proteins

M13 phage＇is a filamentous bacteriophage containing a circular single-stranded DNA molecular, which is surrounded by approximately 2 800 copies of protein PVIII. In addition, there are five copies of each of proteins PVII and PXI in one end, and five copies of each of proteins PVI and PUI on the other. These coat proteins have play an important role in the infection and assembly of M13 phage. With the development of phage display technology, these five coat proteins all can be used in phage display, which plays an increasingly important role in molecular detection and treatment.

Tuning crystal structure and redox potential of NASICON-type cathodes for sodium-ion batteries

Sodium superionic conductor(NASICON)-type compounds have been regarded as promising cathodes for sodium-ion batteries(SIBs)due to their favorable ionic conductivity and robust structural stability.However,their high cost and relatively low energy density restrict their further practical application,which can be tailored by widening the operating voltages with earth-abundant elements such as Mn.Here,we propose a rational strategy of infusing Mn element in NASICON frameworks with sufficiently mobile sodium ions that enhances the redox voltage and ionic migration activity.The optimized structure of Na3.5Mn0.5V1.5(PO4)3/C is achieved and investigated systematically to be a durable cathode(76.6%capacity retention over 5,000 cycles at 20 C)for SIBs,which exhibits high reversible capacity(113.1 mAh·g^−1 at 0.5 C)with relatively low volume change(7.6%).Importantly,its high-areal-loading and temperature-resistant sodium ion storage properties are evaluated,and the full-cell configuration is demonstrated.This work indicates that this Na3.5Mn0.5V1.5(PO4)3/C composite could be a promising cathode candidate for SIBs.