GC-MS/MS Method for Determination and Pharmacokinetics of Main Components of Cang-Ai Volatile Oil in Rat Plasma after Intravenous Administration

[Objectives]To establish a gas chromatography-triple quadrupole mass spectrometry(GC-MS/MS)method based on multiple reaction monitoring(MRM)mode for the analysis of the major components in Cang-ai volatile oil(CAVO).[Methods]An ultrasensitive gas chromatography-tandem mass spectrometry(GC-MS/MS)method was developed and validated for the determination of three highly abundant components in rat plasma samples.Paeonol was used as an internal standard.A multiple reaction monitoring(MRM)model was employed for the quantification of the three major components of CAVO.[Results]The method demonstrated linearity over the range of 0.25 to 50μg/mL with a correlation coefficient(R 2)greater than 0.9998.The lower limit of quantification was 0.25μg/mL.Intra-day and inter-day accuracy and precision were within 15%.Extraction recovery and matrix effect values ranged from 90.1%to 110.6%and 0.1%to 2.1%,respectively.[Conclusions]This method was successfully applied to the simultaneous determination of the three components in high-level CAVO plasma samples,providing a basis for subsequent studies of CAVO.

Method for Predicting the Fatigue Life of Geometrically Discontinuous Structures Under Combined Bending and Torsion

The fatigue damage model based on theory of damage mechanics is capable of predicting the fatigue life under multiaxial loading. Meanwhile, the application of critical plane method in the prediction of multiaxial fatigue life has made certain progress. According to the law of thermodynamics, a new damage evolution equation is developed in the present study to predict the fatigue life of geometrically discontinuous structure under tension-torsion loading based on damage mechanics and the critical plane method. The essence of this approach is tha t the st rain parame ter of the uniaxial nonlinear fatigue damage model is replaced with the equivalent strain, which consists of the releva nt parame ters of the critical plane. However, it is difficult to calculate the stress-strain status and the critical plane position of geometrically dis? continuous structure by theoretical methods because of the existence of stress concentration and the multiaxial nonproportional characteristics. Therefore, a new numerical simulation method is proposed to determine the critical plane of geometrically discontinuous structure under multiaxial loading by means of the finite element method and MATLAB software. The fatigue life of notched specimens subjected to combined bending and torsion is predicted using the proposed met hod, and the result is compared with t hose from the experimen ts and the Manson-Cfiffin law. The comparisons show that the proposed method is superior to the Manson-Coffin law and is capable of reproducing the experimental results reasonably when the geometry of the structure is complex. It completely meets the needs of engineering practice.

A Two-point Method for Multiaxial Fatigue Life Prediction

Fatigue fracture is one of the most common failure modes of engineering compo-nents,and the combined action of geometrie discontinuity and multiaxial loading is more likely to cause severe fatigue damage of components.This work focuses on the fatigue behavior of U-notched Q345 steel specimens with differen t notch sizes under proportional cyclic tension-torsion.Firstly,based on the concept of strain energy,the calculation method of critical plane is given and the equivalent stress of the specified path on the critical plane is extracted to char-acterize the equivalent stress distribution state and the stress gradient effect.Then,based on the high stress volume method and theory of critical distance,a simple method for determining the critical distance is given considering the contribution of stress at the dangerous point and the critical point.In addition,based on the idea of stress-distance normalization,a new stress gradient impact factor is defined and a new method for predicting the multiaxial fatigue life of notched specimens is given.The prediction results of the proposed model,the local stress-strain method and the point method of theory of critical distance are compared with the experimental results.The comparisons show that the prediction results of the proposed model are closer to experimentai life,and the calculation accuracy is higher.