Urbanization is the result of economic and social development,but also an important indicator to measure the level of regional economic and social development. Since the reform and opening up,the level of urbanization...Urbanization is the result of economic and social development,but also an important indicator to measure the level of regional economic and social development. Since the reform and opening up,the level of urbanization in Hebei province has been lagging behind the national average level. The 18 th National Congress of the CPC proposed to develop new urbanization,and this paper evaluated the comprehensive urbanization level of Hebei province combined with new connotation of urbanization. It constructed an evaluation index system of Hebei Province's urbanization comprehensive level,and then used the method of factor analysis with SPSS statistical analysis software to distinguish the major influencing factors. Thirdly,it overlaid the result with spatial pattern,which calculated by the spatial visualization function of ArcGIS software,making the evaluation results presented in a more vivid way. Finally,it analyzed the driving factors of urbanization development in Hebei Province and put forward some suggestions.展开更多
Both the mechanosensitive actin cytoskeleton and caveolae contribute to active processes such as cell migration,morphogenesis,and vesicular trafficking.Although distinct actin components are well studied,how they cont...Both the mechanosensitive actin cytoskeleton and caveolae contribute to active processes such as cell migration,morphogenesis,and vesicular trafficking.Although distinct actin components are well studied,how they contribute to cytoplasmic caveolae,especially in the context of mechano-stress,has remained elusive.Here,we identify two actin-associated mobility stereotypes of caveolin-1(CAV-1)-marked intracellular vesicles,which are characterized as‘dwelling’and‘go and dwelling’.In order to exploit the reason for their distinct dynamics,elongated actin-associated formin functions are perturbed.We find drastically decreased density,increased clustering,and compromised motility of cytoplasmic CAV-1 vesicles resulting from lacking actin nucleator formins by both chemical treatment and RNA silencing of formin genes.Furthermore,hypo-osmosis-stimulated diminishing of CAV-1 is dramatically intensified upon blocking formins.The clustering of CAV-1 vesicles when cells are cultured on soft substrate is also aggravated under formin inhibition condition.Together,we reveal that actin-associated formins are essential for maintaining the dynamic organization of cytoplasmic CAV-1 and importantly its sensitivity upon mechanical challenge.We conclude that tension-controlled actin formins act as a safety valve dampening excessive tension on CAV-1 and safeguarding CAV-1 against mechanical damage.展开更多
基金Supported by Soft Science Project of Hebei Provincial Department of Science and Technology(13456107D)"Triple Talent"Fund Project of Hebei Province in 2011
文摘Urbanization is the result of economic and social development,but also an important indicator to measure the level of regional economic and social development. Since the reform and opening up,the level of urbanization in Hebei province has been lagging behind the national average level. The 18 th National Congress of the CPC proposed to develop new urbanization,and this paper evaluated the comprehensive urbanization level of Hebei province combined with new connotation of urbanization. It constructed an evaluation index system of Hebei Province's urbanization comprehensive level,and then used the method of factor analysis with SPSS statistical analysis software to distinguish the major influencing factors. Thirdly,it overlaid the result with spatial pattern,which calculated by the spatial visualization function of ArcGIS software,making the evaluation results presented in a more vivid way. Finally,it analyzed the driving factors of urbanization development in Hebei Province and put forward some suggestions.
基金This study was supported by grants from the National Natural Science Foundation of China(92054104 and 31970660)CAS–VPST Silk Road Science Fund 2021(GJHZ2021138)+2 种基金Shanghai Municipal Science and Technology Major Project(2019SHZDZX02)Natural Science Foundation of Shanghai(19ZR1463000)‘100 Talents Program’from the Chinese Academy of Sciences.
文摘Both the mechanosensitive actin cytoskeleton and caveolae contribute to active processes such as cell migration,morphogenesis,and vesicular trafficking.Although distinct actin components are well studied,how they contribute to cytoplasmic caveolae,especially in the context of mechano-stress,has remained elusive.Here,we identify two actin-associated mobility stereotypes of caveolin-1(CAV-1)-marked intracellular vesicles,which are characterized as‘dwelling’and‘go and dwelling’.In order to exploit the reason for their distinct dynamics,elongated actin-associated formin functions are perturbed.We find drastically decreased density,increased clustering,and compromised motility of cytoplasmic CAV-1 vesicles resulting from lacking actin nucleator formins by both chemical treatment and RNA silencing of formin genes.Furthermore,hypo-osmosis-stimulated diminishing of CAV-1 is dramatically intensified upon blocking formins.The clustering of CAV-1 vesicles when cells are cultured on soft substrate is also aggravated under formin inhibition condition.Together,we reveal that actin-associated formins are essential for maintaining the dynamic organization of cytoplasmic CAV-1 and importantly its sensitivity upon mechanical challenge.We conclude that tension-controlled actin formins act as a safety valve dampening excessive tension on CAV-1 and safeguarding CAV-1 against mechanical damage.
