The isolation of circulating tumor cells(CTCs)from complex biological samples is of paramount signifi-cance for advancing cancer diagnosis,prognosis,and treatment.However,the low concentration of CTCs and nonspecific ...The isolation of circulating tumor cells(CTCs)from complex biological samples is of paramount signifi-cance for advancing cancer diagnosis,prognosis,and treatment.However,the low concentration of CTCs and nonspecific adhesion of white blood cells(WBCs)present challenges that hinder the efficiency and purity of captured CTCs.Microfluidic-based strategies utilize precise fluid control at the micron level to incorporate specific micro/nanostructures or recognition molecules,enabling effective CTCs separation.Moreover,by employing surface modification designs that exhibit exceptional anti-adhesion properties against WBCs,the purity of isolated CTCs can be further enhanced.This review offers an in-depth explo-ration of recent advancements,challenges,and opportunities associated with microfluidic-based CTCs iso-lation from biological samples.Firstly,we will comprehensively introduce the microfluidic-based strate-gies for achieving high-efficiency CTCs isolation,which includes the morphological design of microchan-nels for physical force-based CTCs isolation and the specific modification of microchannel surfaces for affinity-based CTCs isolation.Subsequently,a review of recent research advances in microfluidic-based high-purity CTCs isolation is presented,focusing on strategies that decrease the nonspecific adhesion of WBCs through surface micro-/nanostructure construction or chemical and biological modification.Finally,we will summarize the article by providing the prospective opportunities and challenges for the future development of microfluidic-based CTCs isolation.展开更多
基金supported by the National Natural Science Foundation of China(Nos.52025132,22005255,21975209,21621091,22021001,T2241022)the National Science Foundation of Fujian Province of China(No.2022J02059)+2 种基金the Fundamental Research Funds for the Central Universities of China(No.20720220085)the 111 Project(Nos.B17027,B16029)the Science and Technology Projects of Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(No.RD2022070601),the New Cornerstone Science Foundation through the XPLORER PRIZE.
文摘The isolation of circulating tumor cells(CTCs)from complex biological samples is of paramount signifi-cance for advancing cancer diagnosis,prognosis,and treatment.However,the low concentration of CTCs and nonspecific adhesion of white blood cells(WBCs)present challenges that hinder the efficiency and purity of captured CTCs.Microfluidic-based strategies utilize precise fluid control at the micron level to incorporate specific micro/nanostructures or recognition molecules,enabling effective CTCs separation.Moreover,by employing surface modification designs that exhibit exceptional anti-adhesion properties against WBCs,the purity of isolated CTCs can be further enhanced.This review offers an in-depth explo-ration of recent advancements,challenges,and opportunities associated with microfluidic-based CTCs iso-lation from biological samples.Firstly,we will comprehensively introduce the microfluidic-based strate-gies for achieving high-efficiency CTCs isolation,which includes the morphological design of microchan-nels for physical force-based CTCs isolation and the specific modification of microchannel surfaces for affinity-based CTCs isolation.Subsequently,a review of recent research advances in microfluidic-based high-purity CTCs isolation is presented,focusing on strategies that decrease the nonspecific adhesion of WBCs through surface micro-/nanostructure construction or chemical and biological modification.Finally,we will summarize the article by providing the prospective opportunities and challenges for the future development of microfluidic-based CTCs isolation.
