In the present study, ultrasound-mediated microbubble destruction （UMMD） alone and combined with liposome technology was used as a novel nonviral technique to transfect a Nogo receptor （Nogo-R） shRNA plasmid （pNo...In the present study, ultrasound-mediated microbubble destruction （UMMD） alone and combined with liposome technology was used as a novel nonviral technique to transfect a Nogo receptor （Nogo-R） shRNA plasmid （pNogo-R shRNA） into neural stem cells （NSCs）. Using green fluorescent protein as a reporter gene, transfection efficiency of NSCs was significantly higher in the group transfected with UMMD combined with liposomes compared with that of the group transfected with UMMD or liposomes alone, and did not affect cell vitality. In addition, Nogo-R mRNA and protein expression was dramatically decreased in the UMMD combined with liposome-mediated group compared with that of other groups after 24 hours of transfection. The UMMD technique combined with liposomes is a noninvasive gene transfer method, which showed minimal effects on cell viability and effectively increased transfer of Nogo-R shRNA into NSCs.展开更多
In this paper,we derive a nonlinearequation of corneal asphericity (Q) usingthe tangential radius of curvature (rt) on every semi-meridian. We transform the nonlinear equation into the linear equation and then obtain ...In this paper,we derive a nonlinearequation of corneal asphericity (Q) usingthe tangential radius of curvature (rt) on every semi-meridian. We transform the nonlinear equation into the linear equation and then obtain theQ-value of cor-neal semi-meridianby the linear regression method.We find the 360 semi-meridional variation rule of theQ-value us-ing polynomial function. Furthermore, we construct a new 3D corneal model and present a more realistic model of shape of the anterior corneal surface.展开更多
基金supported by the Natural Science Foundation of Chongqing (Ultrasound microbubble combining with NgR-RNAi promoted neural stem cells repair of spinal cord injury in rats), No. 2008BB5223
文摘In the present study, ultrasound-mediated microbubble destruction （UMMD） alone and combined with liposome technology was used as a novel nonviral technique to transfect a Nogo receptor （Nogo-R） shRNA plasmid （pNogo-R shRNA） into neural stem cells （NSCs）. Using green fluorescent protein as a reporter gene, transfection efficiency of NSCs was significantly higher in the group transfected with UMMD combined with liposomes compared with that of the group transfected with UMMD or liposomes alone, and did not affect cell vitality. In addition, Nogo-R mRNA and protein expression was dramatically decreased in the UMMD combined with liposome-mediated group compared with that of other groups after 24 hours of transfection. The UMMD technique combined with liposomes is a noninvasive gene transfer method, which showed minimal effects on cell viability and effectively increased transfer of Nogo-R shRNA into NSCs.
文摘In this paper,we derive a nonlinearequation of corneal asphericity (Q) usingthe tangential radius of curvature (rt) on every semi-meridian. We transform the nonlinear equation into the linear equation and then obtain theQ-value of cor-neal semi-meridianby the linear regression method.We find the 360 semi-meridional variation rule of theQ-value us-ing polynomial function. Furthermore, we construct a new 3D corneal model and present a more realistic model of shape of the anterior corneal surface.
