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三酶级联催化L-苏氨酸生产L-2-氨基丁酸 被引量:4
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作者 付妍 张君轩 +5 位作者 付雪蓉 解雨晨 任泓宇 刘佳 陈修来 刘立明 《生物工程学报》 CAS CSCD 北大核心 2020年第4期782-791,共10页
L-2-氨基丁酸(L-ABA)是一种重要的化工原材料和手性医药中间体,为了实现L-ABA的高效生产,本研究在大肠杆菌EscherichiacoliBL21(DE3)中分别表达大肠杆菌来源的苏氨酸脱氨酶(Threonine deaminase,TD)、苏云金芽孢杆菌来源的亮氨酸脱氢酶(... L-2-氨基丁酸(L-ABA)是一种重要的化工原材料和手性医药中间体,为了实现L-ABA的高效生产,本研究在大肠杆菌EscherichiacoliBL21(DE3)中分别表达大肠杆菌来源的苏氨酸脱氨酶(Threonine deaminase,TD)、苏云金芽孢杆菌来源的亮氨酸脱氢酶(Leucine dehydrogenase,LDH)和博伊丁假丝酵母来源的甲酸脱氢酶(Formatedehydrogenase,FDH),构建体外级联酶催化反应实现L-苏氨酸向L-ABA的转化,体系中TD、LDH和FDH添加最适比例为1∶1∶0.2。为了简化生产工艺,将3种酶在一株菌E. coli 3FT+L中共表达并实现上述配比,在30 L发酵罐中用E. coli 3FT+L全细胞转化12 h,L-ABA的产量为68.5 g/L,底物L-苏氨酸的摩尔转化率达到99.0%。该工艺路线绿色高效,为未来大规模生产L-ABA提供借鉴。 展开更多
关键词 L-2-氨基丁酸 三酶级联 共表达重组菌 全细胞转化 L-苏氨酸
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半完备余代数上余模范畴的黏合
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作者 付雪荣 姚海楼 《中国科学:数学》 CSCD 北大核心 2018年第4期483-496,共14页
本文主要研究半完备余代数上余模范畴的黏合,证明黏合中的范畴是余模范畴当且仅当它是由半完备余代数的余幂等子余代数诱导的黏合,进一步还将此结果应用到Morita-Takeuchi关系余代数和余模复形范畴上.
关键词 黏合 TTF(Torsion-Torsion free)-三元组 余幂等子余代数 余模复形范畴
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On Gorenstein coalgebras
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作者 xuerong fu Hailou YAO 《Frontiers of Mathematics in China》 SCIE CSCD 2016年第4期845-867,共23页
We investigate the comodule representation category over the Morita-Takeuchi context coalgebra F and study the Gorensteinness of F. Moreover, we determine explicitly all Gorenstein injective comodules over the Morita-... We investigate the comodule representation category over the Morita-Takeuchi context coalgebra F and study the Gorensteinness of F. Moreover, we determine explicitly all Gorenstein injective comodules over the Morita-Takeuchi context coalgebra F and discuss the localization in Gorenstein coalgebras. In particular, we describe its Gabriel quiver and carry out some examples when the Morita-Takeuchi context coalgebra is basic. 展开更多
关键词 Gorenstein coalgebra Gorenstein injective comodule Gabriel quiver
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Metabolic energy variation of yeast affects its antioxidant properties in beer brewing
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作者 Jinjing Wang xuerong fu +4 位作者 Mengqi Li Feiyun Zheng Chengtuo Niu Chunfeng Liu Qi Li 《Systems Microbiology and Biomanufacturing》 2021年第3期311-322,共12页
Oxidation destroys the flavor and freshness of the beer,and yeast with improved antioxidant activity would benefit the flavor stability of the beer.The aim of this work was to study the antioxidant networks of lager y... Oxidation destroys the flavor and freshness of the beer,and yeast with improved antioxidant activity would benefit the flavor stability of the beer.The aim of this work was to study the antioxidant networks of lager yeast for brewing industry.Through transcriptome and metabolome analysis,we were able to map changes in anti-oxidant pathways and transcription of genes in lager yeast.Results suggested that metabolic energy variation in yeast might be the main reason for the improved anti-oxidant capacity.Lower metabolic rate kept the yeast at relatively lower respiratory status at the end of fermentation,thereby prolonging the lifespan and potentially supporting its usage in serial fermentations.Up-regulation of mannose synthesis and hexose transport strengthened the cell structure,which may have contributed to the anti-oxidant capacity of yeast.A deeper understanding of the globally regulation pattern of anti-oxidation in lager yeast is expected to support the design of strain development strategies for improved anti-oxidant capacity of yeast for fermentation industry. 展开更多
关键词 Lager yeast ANTI-OXIDATION Metabolic energy variation ROBUSTNESS
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