mRNA profiles of cytokine receptors in unstimulated peripheral blood mononuclear cells from patients with chronic idiopathic urticaria

This present study was aimed to investigate the roles of the receptors of Thl/Th2 cytokines and chemokines in lhe pathogenesis of chronic idiopathic urticaria （CIU）. Thirty patients with CIU, 30 patients with dermographism and 30 healthy controls were randomly enrolled. Reverse transcription-PCR （RT-PCR） was used to analyze the mRNA of cytokine receptors in peripheral blood mononuclear cells （PBMCs）. The mRNA levels of tumor necro- sis factor receptor （TNFR）, interferon-7 receptor （IFN-yR）, and interleukin-10 receptor （IL-IOR） were statistically increased in the CIU group （P 〈 0.05）, while IL-2R, IL-4R, IL-6R, and IL-13R showed no significant differences between the CIU and other groups. The mRNA levels of CCR3 and CCR6 were statistically increased in the CIU group （P 〈 0.05）. The toll-like receptor 2 （TLR2） mRNA level was significantly lower in the CIU group than the healthy control group （P 〈 0.05）. These findings indicate that the regulation of mRNA of TNFR, IFN-γR, IL-IOR, CCR3, CCR6 and TLR2 may be involved in the pathogenesis of CIU.

缺血性心肌病的预后及不良预后的危险因素分析

目的探讨缺血性心肌病(ICM)的预后特点,并分析其不良预后的危险因素。方法该研究为回顾性研究。收集天津市胸科医院心脏重症监护室2019年12月至2022年6月收治的慢性心力衰竭(心衰)急性失代偿患者的临床资料。根据心衰病因将入选者分为ICM组及非缺血性心肌病(NICM)组,然后进一步根据是否发生主要或次要终点事件对ICM组患者进行亚组分析。收集入选者的基线临床资料及随访结果。主要终点事件为全因死亡和/或因心衰再入院的复合终点,次要终点事件为心原性死亡。采用Kaplan-Meier生存曲线比较ICM组和NICM组患者的生存差异;采用单因素及多因素Cox回归模型分析ICM患者主要及次要终点事件的影响因素。结果纳入患者271例,男186例(68.6%),年龄66.0(56.0,75.0)岁,其中ICM组135例,NICM组136例。与NICM组比较,ICM组患者年龄更大,心率更快,体重指数更大,合并高血压、糖尿病、慢性肾病以及卒中或外周血管疾病者占比更高,左心室舒张末期内径、右心室舒张末期前后径更大,肺动脉收缩压、左心室射血分数更高(均P<0.05)。Kaplan-Meier生存分析结果显示,与NICM组比较,ICM组患者无主要终点事件生存率(Log-rank P=0.009)和无次要终点事件生存率(Log-rank P=0.037)更低。多因素Cox回归分析结果显示,甘油三酯(TG)/高密度脂蛋白胆固醇(HDL-C)比值升高、中性粒细胞百分比升高是ICM患者发生主要及次要终点事件的独立危险因素(均P<0.05)。结论与NICM患者比较,ICM患者的预后更差,TG/HDL-C比值及中性粒细胞百分比升高是ICM不良预后的独立危险因素。