Cucumber selective breeding over recent decades has dramatically increased productivity and quality,but the genomic characterizations and changes associated with this breeding history remain unclear.Here,we analyzed t...Cucumber selective breeding over recent decades has dramatically increased productivity and quality,but the genomic characterizations and changes associated with this breeding history remain unclear.Here,we analyzed the genome resequencing data of 56 artificially selected cucumber inbred lines that exhibit various phenotypes to detect trait-associated sequence variations that reflect breeding improvement.We found that the 56 cucumber lines could be assigned to group 1 and group 2,and the two groups formed a distinctive genetic structure due to the breeding history involving hybridization and selection.Differentially selected regions were identified between group 1 and group 2,with implications for genomic-selection breeding signatures.These regions included known quantitative trait loci or genes that were reported to be associated with agronomic traits.Our results advance knowledge of cucumber genomics,and the 56 selected inbred lines could be good germplasm resources for breeding.展开更多
Soil salinization is a major threat to cucumbers grown under protected cultivation.Under stressful environments,calcineurin B-like proteins(CBLs)can sense and bind Ca2+signals and regulate CBL-interacting protein kina...Soil salinization is a major threat to cucumbers grown under protected cultivation.Under stressful environments,calcineurin B-like proteins(CBLs)can sense and bind Ca2+signals and regulate CBL-interacting protein kinases(CIPKs)to transmit signals and induce cellular responses.Although CBL-CIPK modules play central roles in plant development and response to various abiotic stresses in Arabidopsis,little is known about their functions in cucumber.In this study,we demonstrate that CsCBL4 interacts with CsCIPK6,which exhibited similar responses to salt stress in cucumber.Furthermore,salt stress resulted in greater accumulation of CsCBL4 and CsCIPK6.Comprehensive phenotype analysis demonstrated that silencing CsCBL4 or CsCIPK6 reduced the salt tolerance of cucumber,and overexpression of CsCBL4 increased the salt tolerance of Arabidopsis.Collectively,these results indicate that the CsCBL4-CsCIPK6 module plays an important role in the resistance of cucumber to salt stress.The information provides insights for the genetic breeding of salt tolerance in cucumber in the future.展开更多
基金supported by the National Key Research and Development Program of China(2018YFD1000800)the National Natural Science Foundation of China(31830080)+1 种基金the Beijing Municipal Natural Science Foundation(6184043)the Project of Beijing Agricultural Innovation Consortium(BAIC01).
文摘Cucumber selective breeding over recent decades has dramatically increased productivity and quality,but the genomic characterizations and changes associated with this breeding history remain unclear.Here,we analyzed the genome resequencing data of 56 artificially selected cucumber inbred lines that exhibit various phenotypes to detect trait-associated sequence variations that reflect breeding improvement.We found that the 56 cucumber lines could be assigned to group 1 and group 2,and the two groups formed a distinctive genetic structure due to the breeding history involving hybridization and selection.Differentially selected regions were identified between group 1 and group 2,with implications for genomic-selection breeding signatures.These regions included known quantitative trait loci or genes that were reported to be associated with agronomic traits.Our results advance knowledge of cucumber genomics,and the 56 selected inbred lines could be good germplasm resources for breeding.
基金supported by the Hainan Provincial Joint project of Sanya Yazhou Bay science and technology city (320LH042)Sanya institute of China Agricultural University (SYND-2021-18,SYND-2022-20)the Construction of Beijing Science and Technology Innovation and Service Capacity in Top Subjects (CEFF-PXM2019_014207_000032).
文摘Soil salinization is a major threat to cucumbers grown under protected cultivation.Under stressful environments,calcineurin B-like proteins(CBLs)can sense and bind Ca2+signals and regulate CBL-interacting protein kinases(CIPKs)to transmit signals and induce cellular responses.Although CBL-CIPK modules play central roles in plant development and response to various abiotic stresses in Arabidopsis,little is known about their functions in cucumber.In this study,we demonstrate that CsCBL4 interacts with CsCIPK6,which exhibited similar responses to salt stress in cucumber.Furthermore,salt stress resulted in greater accumulation of CsCBL4 and CsCIPK6.Comprehensive phenotype analysis demonstrated that silencing CsCBL4 or CsCIPK6 reduced the salt tolerance of cucumber,and overexpression of CsCBL4 increased the salt tolerance of Arabidopsis.Collectively,these results indicate that the CsCBL4-CsCIPK6 module plays an important role in the resistance of cucumber to salt stress.The information provides insights for the genetic breeding of salt tolerance in cucumber in the future.
