Highly contagious COVID-19 disease is caused by a novel severe acute respiratory syndrome coronavirus 2(SARSCoV-2),which poses a serious threat to global public health.Therefore,the development of a fast and reliable ...Highly contagious COVID-19 disease is caused by a novel severe acute respiratory syndrome coronavirus 2(SARSCoV-2),which poses a serious threat to global public health.Therefore,the development of a fast and reliable method for the detection of SARS-CoV-2 is an urgent research need.The Fe3O4@SiO2–Au is enriched with a variety of functional groups,which can be used to fabricate a sensitive electrochemical biosensor by biofunctionalization with angiotensin-converting enzyme 2(ACE2).Accordingly,we developed a novel electrochemical sensor by chemically modifying a glassy carbon electrode(GCE)with Fe3O4@SiO2–Au nanocomposites(hereafter Fe_(3)O_(4)@SiO_(2)–Au/GCE)for the rapid detection of S-protein spiked SARS-CoV-2 by electrochemical impedance spectroscopy(EIS).The new electrochemical sensor has a low limit detection(viz.,4.78 pg/mL)and a wide linear dynamic range(viz.,0.1 ng/mL to 10μg/mL)for detecting the EIS response signal of S-protein.The robust Fe_(3)O_(4)@SiO_(2)–Au/GCE biosensor has high selectivity,stability,and reproducibility for the detection of Sprotein with good recovery of saliva samples.展开更多
基金the financial support from the National Key Research and Development Program of China(2022YFE0110100)Key Science and Technology Projects of Anhui Province(202003a07020004)+1 种基金National Natural Science Foundation of China(21777164)Program of Distinguished Professor in B&R Countries(DL20180052)abidance at the Hefei University of Technology.
文摘Highly contagious COVID-19 disease is caused by a novel severe acute respiratory syndrome coronavirus 2(SARSCoV-2),which poses a serious threat to global public health.Therefore,the development of a fast and reliable method for the detection of SARS-CoV-2 is an urgent research need.The Fe3O4@SiO2–Au is enriched with a variety of functional groups,which can be used to fabricate a sensitive electrochemical biosensor by biofunctionalization with angiotensin-converting enzyme 2(ACE2).Accordingly,we developed a novel electrochemical sensor by chemically modifying a glassy carbon electrode(GCE)with Fe3O4@SiO2–Au nanocomposites(hereafter Fe_(3)O_(4)@SiO_(2)–Au/GCE)for the rapid detection of S-protein spiked SARS-CoV-2 by electrochemical impedance spectroscopy(EIS).The new electrochemical sensor has a low limit detection(viz.,4.78 pg/mL)and a wide linear dynamic range(viz.,0.1 ng/mL to 10μg/mL)for detecting the EIS response signal of S-protein.The robust Fe_(3)O_(4)@SiO_(2)–Au/GCE biosensor has high selectivity,stability,and reproducibility for the detection of Sprotein with good recovery of saliva samples.
