Despite multiple virus outbreaks over the past decade,including the devastating coronavirus disease 2019(COVID-19)pandemic,the lack of accurate and timely diagnosis and treatment technologies has wreaked havoc on glob...Despite multiple virus outbreaks over the past decade,including the devastating coronavirus disease 2019(COVID-19)pandemic,the lack of accurate and timely diagnosis and treatment technologies has wreaked havoc on global biosecurity.The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated proteins(Cas)system has the potential to address these critical needs for tackling infectious diseases to detect viral nucleic acids and inhibit viral replication.This review summarizes how the CRISPR/Cas system is being utilized for the treatment and diagnosis of infectious diseases with the help of biosafety materials and highlights the design principle and in vivo and in vitro efficacy of advanced biosafety materials used to deal with virus attacks.展开更多
基金supported by the Beijing Municipal Natural Science Foundation(No.7214302)the Natural Science Foundation of Guangdong Province(2019A1515010776)+4 种基金the Beijing-Tianjin-Hebei Basic Research Cooperation Project(19JCZDJC64100)the Beijing Nova Program from Beijing Municipal Science&Technology Commission(Z201100006820005)the National Natural Science Foundation of China(31871003 and 32001008)the National Key R&D Program of China(2021YFE0106900)the Open Fund of Key Laboratory of Medicine Chemistry and Molecular Diagnosis(Hebei University),Ministry of Education of China,and the Young Elite Scientist Sponsorship Program of Beijing Association for Science and Technology(2020-2022).
文摘Despite multiple virus outbreaks over the past decade,including the devastating coronavirus disease 2019(COVID-19)pandemic,the lack of accurate and timely diagnosis and treatment technologies has wreaked havoc on global biosecurity.The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated proteins(Cas)system has the potential to address these critical needs for tackling infectious diseases to detect viral nucleic acids and inhibit viral replication.This review summarizes how the CRISPR/Cas system is being utilized for the treatment and diagnosis of infectious diseases with the help of biosafety materials and highlights the design principle and in vivo and in vitro efficacy of advanced biosafety materials used to deal with virus attacks.
