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Effects of L-3-n-butylphthalide on caspase-3 and nuclear factor kappa-B expression in primary basal forebrain and hippocampal cultures after beta-amyloid peptide 1-42 treatment 被引量:3
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作者 Ruixia Wang Yong Zhang +12 位作者 Liangliang Jiang Guozhao Ma Qingxi Fu Jialong Li Peng Yan Lunqian Shen yabo feng Chunxia Li Zaiying Pang Yuanxiao Cui Chunfu Chen Yifeng Du Zhaokong Liu 《Neural Regeneration Research》 SCIE CAS CSCD 2009年第4期252-257,共6页
BACKGROUND: L-3-n-butylphthalide (L-NBP) can inhibit phosphorylation of tau protein and reduce the neurotoxicity of beta-amyloid peptide 1-42 (Aβ1-42). OBJECTIVE: To observe the neuroprotective effects of L-NBP... BACKGROUND: L-3-n-butylphthalide (L-NBP) can inhibit phosphorylation of tau protein and reduce the neurotoxicity of beta-amyloid peptide 1-42 (Aβ1-42). OBJECTIVE: To observe the neuroprotective effects of L-NBP on caspase-3 and nuclear factor kappa-B (NF- K B) expression in a rat model of Alzheimer's disease. DESIGN, TIME AND SETTING: A cell experiment was performed at the Central Laboratory of Provincial Hospital affiliated to Shandong University between January 2008 and August 2008. MATERIALS: L-NBP (purity 〉 98%) was provided by Shijiazhuang Pharma Group NBP Pharmaceutical Company Limited. Aβ1-42, 3-[4,5-dimethylthiazolo-2]-2,5 iphenyltetrazolium bromide (MTT), and rabbit anti-Caspase-3 polyclonal antibody were provided by Cell Signaling, USA; goat anti-choactase and rabbit anti-NF- kB antibodies were provided by Santa Cruz, USA. METHODS: Primary cultures were generated from rat basal forebrain and hippocampal neurons at 17 or 19 days of gestation. The cells were assigned into five groups: the control group, the Aβ1-42 group (2 μmol/L), the Aβ1-42 + 0.1 μmol/L L-NBP group, the Aβ1-42 + 1 μ mol/L L-NBP group, and the Aβ1-42 + 10μmol/L L-NBP group. The neurons were treated with Aβ1-42 (2 μmol/L) alone or in combination with L-NBP (0.1, 1, 10 μmol/L) for 48 hours. Cells in the control group were incubated in PBS. MAIN OUTCOME MEASURES: Morphologic changes were evaluated using inverted microscopy, viability using the M-I-I- method, and the changes in caspase-3 and NF- k B expression using Western blot. RESULTS: Induction with Aβ1-42 for 48 hours caused cell death and soma atrophy, and increased caspase-3 and NF- K B expression (P 〈 0.05). L-NBP blocked these changes in cell morphology, decreased caspase-3 and NF- k B expression (P 〈 0.05), and improved cell viability, especially at the high dose (P 〈 0.05). CONCLUSION: AI3^-42 is toxic to basal forebrain and hippocampal primary neurons; L-NBP protects against this toxicity and inhibits the induction of caspase-3 and NF- K B expression. 展开更多
关键词 L-3-n-butylphthalide cholinergic neurons beta-amyloid peptide 1-42 CASPASE-3 nuclear factor kappa-B
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Ultra-wide-dynamic-range gas sensing by optical pathlength multiplexed absorption spectroscopy 被引量:5
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作者 XIUTAO LOU yabo feng +1 位作者 SHUNHU YANG YONGKANG DONG 《Photonics Research》 SCIE EI CAS CSCD 2021年第2期193-201,共9页
Laser absorption spectroscopy(LAS) has become the most widely used laser spectroscopic technique for gas detection due to its capability of accurate quantification and straightforward operation. However, since resolvi... Laser absorption spectroscopy(LAS) has become the most widely used laser spectroscopic technique for gas detection due to its capability of accurate quantification and straightforward operation. However, since resolving weak absorption and averting over-absorption are always mutually exclusive, the dynamic range of the LAS-based gas sensor is limited and insufficient for many applications in fundamental study and industry. To overcome the limitation on the dynamic range, this article reports optical pathlength(OPL) multiplexed absorption spectroscopy using a gas cell having multiple internal reflections. It organically fuses together the transmission and reflection operation modes: the former directly uses the entire OPL of the gas cell, while the latter interrogates different internal short OPLs by optical interferometry, yielding >100-fold OPL variation. The achieved dynamic range is more than 6 orders of magnitude that surpasses other LAS techniques by 2–3 orders of magnitude. The proposed method promotes a novel way for the development of large-dynamic-range spectroscopic gas sensors for fundamental studies and industrial applications. 展开更多
关键词 OPTICAL ABSORPTION DYNAMIC
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