双荧光标记的人高骨转移肺腺癌细胞株的建立及其转录组学特征分析

背景与目的骨是肺腺癌常见的转移部位,但肺腺癌骨转移的机制尚不明确。目前肺腺癌骨转移机制研究缺乏易于示踪且稳定高骨转移的肺腺癌细胞模型,因此,本研究旨在建立绿色荧光蛋白(green f luorescent protein,GFP)和萤火虫荧光素酶(firefly luciferase,LUC)双标记的人高骨转移肺腺癌细胞株,为肺腺癌骨转移的研究提供新的实验工具。方法人肺腺癌细胞系A549-GFP-LUC经左心室注射至裸鼠体内构建骨转移模型,经连续3次体内驯化,获取人高骨转移肺腺癌细胞株A549-GFP-LUC-BM3;CCK-8(cell counting kit-8)、克隆形成实验比较A549-GFP-LUC-BM3细胞株和亲本细胞的体外增殖能力,划痕实验、Transwell实验以及Western blot比较迁移和侵袭能力;并进一步将A549-GFP-LUC-BM3细胞和亲本细胞行测序转录组学分析。结果成功建立人高骨转移肺腺癌细胞A549-GFP-LUC-BM3,相较于亲本细胞,该细胞骨转移发生率显著提高,且体外增殖、迁移和侵袭能力显著增强。转录组学测序结果显示,相较于亲本细胞,A549-GFP-LUC-BM3细胞中共筛选到差异基因2954个,其中1021个基因上调,1933个基因下调;基因本体(Gene Ontology,GO)功能富集显示差异基因主要定位于细胞外周、质膜以及细胞外基质等细胞组分,分子功能主要富集在信号受体结合、钙离子结合和细胞外基质结构成分等,生物过程富集在细胞黏附和生物黏附等;京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析显示差异基因在细胞色素P450(cytochrome P450,CYP)对外源性物质的代谢、视黄醇代谢、细胞黏附分子、CYP对药物代谢、类固醇激素的生物合成以及核因子κB(nuclear factor kappa B,NF-κB)信号通路上显著富集。结论成功建立GFP和LUC双标记的人高骨转移肺腺癌细胞株,该细胞株在生物学行为水平和转录组测序水平均提示具有高骨转移潜能。

Tissue distribution of cadmium and its effect on reproduction in Spodoptera exigua

Vegetable fields are often contaminated by heavy metals,and Spodoptera exigua is a major vegetable pest which is stressed by heavy metals mainly by feeding.In this study,cadmium accumulation in the tissues of S.exigua exposed to cadmium and its effects on the growth and development of the parents and the offspring were investigated.Under the stress of different concentrations of cadmium(0.2,3.2,and 51.2 mg kg^(-1)),the cadmium content in each tissue of S.exigua increased in a dose-dependent manner.At the larval stage,the highest cadmium accumulation was found in midgut in all three cadmium treatments,but at the adult stage,the highest cadmium content was found in fat body.In addition,the cadmium content in ovaries was much higher than in testes.When F1S.exigua was stressed by cadmium and the F_(2)generation was not fed a cadmium-containing diet,the larval survival,pupation rate,emergence rate and fecundity of the F_(2)generation were significantly reduced in the 51.2 mg kg^(-1)treatment compared to the corresponding F1generation.Even in the F_(2)generation of the 3.2 mg kg^(-1)treatment,the fecundity was significantly lower than in the parental generation.The fecundity of the only-female stressed treatment was significantly lower than that of the only-male stressed treatment at the 3.2 and 51.2 mg kg^(-1)cadmium exposure levels.When only mothers were stressed at the larval stage,the fecundity of the F_(2)generation was significantly lower than that of the F1generation in the 51.2 mg kg^(-1)treatment,and it was also significantly lower than in the 3.2 and 0.2 mg kg^(-1)treatments.The results of our study can provide useful information for forecasting the population increase trends under different heavy metal stress conditions and for the reliable environmental risk assessment of heavy metal pollution.

Coordination structure regulation in non-flammable electrolyte enabling high voltage lithium electrochemistry

High-voltage battery systems bring significant increases in energy density but are also accompanied by fast degradation of electrochemical performance and serious safety issues.Herein,Li^(+)coordination structure regulation was conducted to formulate a non-flammable electrolyte,which consists of 1.5 M lithium bis(fluor sulfonyl)imide(LiFSI)in triethyl phosphate and methyl 2,2,2-trifluoromethyl carbonate(FEMC).The renamed TEP-FEMC-FEC(TFF)electrolyte exhibits an FSI^(−)-dominated solvation structure contributed by the weakly-solvating ability of FEMC.The generated inorganic-rich interfacial layers are conducive to stabilizing the phase transition of high-voltage cathodes while suppressing the dendritic growth on lithium metal or co-intercalation behavior in graphite anode.This TFF electrolyte enables LiCoO_(2)||Li batteries to achieve capacity maintenance over 79%after 400 cycles with high-rate of 5 C at an ultra-high voltage of 4.6 V,and an outstanding capacity exceeding 100 mA h g^(−1)even at a super-high current density of 20 C.Additionally,the Ah-level LiCoO_(2)||graphite pouch cells also exhibit high capacity retention and satisfactory safety performance even under fast charging.This work provides a novel research direction for the pursuit of high energy density non-flammable electrolytes.

Rationalizing Na-ion solvation structure by weakening carbonate solvent coordination ability for high-voltage sodium metal batteries

Commercial carbonate-based electrolytes feature highly reactive activities with alkali metals,yielding low Coulombic efficiencies and poor cycle life in lithium metal batteries,which possess much higher chemical activity in the rising star sodium metal batteries.To be motivated,we have proposed that decreasing the solvent solvation ability in carbonate-based electrolytes stepwise could enable longterm stable cycling of high-voltage sodium metal batteries.As the solvation capacity reduces,more anions are enticed into the solvation sheath of Na^(+),resulting in the formation of the more desirable interphase layers on the surface of the anode and the cathode.The inorganic-dominated interphases allow highly efficient Na^(+)deposition/stripping processes with a lower rate of dead sodium generation,as well as maintain a stable structure of the high-voltage cathode material.Specifically,the assembled Na||Na_(3)V_(2)(PO_(4))_(2)F_(3)battery exhibits an accelerated ion diffusion kinetics and achieves a higher capacity retention of 85.9%with during the consecutive 200 cycles under the high voltage of 4.5 V.It is anticipated that the tactics we have proposed could be applicable in other secondary metal battery systems as well.

MiR-183-5p promotes the progression of non-small cell lung cancer through targeted regulation of FOXO1

Objective To investigate miR-183-5p targeting to forkhead box protein O1(FOXO1)and its corresponding effect on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)of non-small cell lung cancer(NSCLC)cells.Methods NSCLC tissues and adjacent normal tissues from 60 patients with NSCLC adenocarcinoma were obtained via pathological biopsy or intraoperative resection.Several cell lines were cultured in vitro,including the human normal lung epithelial cell line BEAS-2B and human NSCLC cell lines A549,SPCA-1,PC-9,and 95-D.miR-183-5p and FOXO1 mRNA expression in tissues and cells were detected by qRT-PCR;the corresponding correlations in NSCLC tissues were analyzed using the Pearson test,and the relationship between miR-183-5p expression and clinicopathological parameters was analyzed.The miR-183-5p-mediated regulation of FOXO1 was verified by bioinformatics prediction alongside double luciferase,RNA-binding protein immunoprecipitation(RIP)assay,and pull-down experiments.A549 cells were divided into control,anti-miR-NC,anti-miR-183-5p,miR-NC,miR-183-5p,miR-183-5p+pcDNA3.1,and miR-183-5p+pcDNA3.1-FOXO1 groups.Cell proliferation,invasion,migration,apoptosis,and cell cycle distribution were detected using an MTT assay,clone formation assay,Transwell assay,scratch test,and flow cytometry,respectively.The expression of EMT-related proteins in the cells was analyzed by western blotting.The effect of miR-185-3p silencing on the development of transplanted tumors was detected by analyzing tumor formation in nude mice.Results miR-183-5p expression was significantly higher in NSCLC tissues and cells than in adjacent normal tissues,whereas FOXO1 mRNA expression was significantly down-regulated.There was a significant negative correlation between miR-183-5p and FOXO1 mRNA in NSCLC tissues(P<0.05).Additionally,the expression of miR-183-5p was significantly correlated with tumor size,tumor differentiation,and tumor-node-metastasis stage in patients with NSCLC(P<0.05).miR-183-5p targeted and inhibited FOXO1 expression.Compared to the anti-miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the anti-miR-183-5p group,whereas the protein expression of E-cadherin andα-catenin and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion were significantly lower in the anti-miR-183-5p group(P<0.05).Compared to the miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells in the miR-183-5p group were significantly higher,whereas the E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly lower;furthermore,the frequency of colony formation and invasion were significantly higher in the miR-183-5p group(P<0.05).Compared with the miR-183-5p+pcDNA3.1 group,the OD value,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group,whereas E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion was significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group(P<0.05).Overall,silencing miR-185-3p inhibited the growth of transplanted tumors and promoted FOXO1 expression.Conclusion Overexpression of miR-183-5p can inhibit apoptosis and promote the proliferation,migration,invasion,and EMT,of NSCLC cells by down-regulating FOXO1 expression.

楼板对RC空间框架结构抗连续倒塌性能影响试验研究

为研究楼板对RC空间框架结构抗连续倒塌性能的影响作用,文章以汶川地震中完全倒塌的漩口中学教学楼为原型结构,设计3个单层2×2跨1/3缩尺模型,包括2个带楼板子结构和1个纯框架子结构,通过拟静力加载试验研究RC框架结构在拆除中柱状态下的连续倒塌破坏过程。试验结果表明:纯框架子结构首先进入梁机制阶段,框架梁内产生轴向压力,产生'压拱效应',在梁端部截面抗弯承载力丧失后,进入悬链线机制阶段,依靠梁内部钢筋拉结力抵抗上部荷载。对比分析带楼板子结构与纯框架子结构试验结果,由于楼板的'薄膜效应'使梁机制峰值承载力提高了145%,悬链线机制峰值承载力提高了75%,楼板显著提高了梁机制和悬链线机制的抗连续倒塌能力。增加板厚增加梁板的'压拱效应',极大提高梁机制承载力,然而大变形下梁板开始不协同作用,板发生冲切破坏,降低了悬链线阶段承载力和延性。

基于共旋坐标和力插值纤维单元的RC框架结构连续倒塌构造方法

悬链机制会使钢筋混凝土框架结构产生有助于抵抗连续倒塌的附加承载能力,对结构抗连续倒塌能力至关重要。悬链机制处于几何大变形和材料非线性下降段的状态下,需要同时考虑材料非线性和几何非线性,因此对数值分析模型提出了更高的要求。为了解决基于力插值的纤维单元同时处理材料非线性和几何非线性的问题,该文采用基于共旋坐标法,提出了一种基于共旋坐标法的力插值纤维单元。该单元在形成中把变形体和刚体分开,局部坐标系的变形体内采用纤维划分考虑材料非线性,然后加上刚体位移,从局部坐标系到整体坐标系的转换中采用共旋坐标法以考虑几何非线性,给出了二维单元形成原理及非线性求解过程。实例分析结果表明基于共旋坐标法的力插值纤维单元能够较准确的模拟RC框架结构连续倒塌,梁机制阶段主要是材料非线性起控制作用,悬链线机制阶段主要是几何非线性起控制作用。

基于FEMA P-58的RC框架结构抗震及减隔震性能评估

该文采用FEMA P-58理论进行RC框架结构抗震及减隔震性能评估。设计了一栋典型多层RC框架结构,并在此基础上添加屈曲约束支撑以及隔震支座形成BRB-框架结构与隔震框架结构,采用OpenSees软件建立了3个结构的有限元模型,选取合适的地震动记录并调幅,对三个结构进行了非线性结构响应分析。针对多遇地震、基本地震、罕遇地震、极罕遇地震4个地震动强度,使用FEMA P-58理论中基于强度的方法,对三个结构在四个地震强度下进行性能评估,并针对各性能指标的评估结果进行对比分析。地震损失评估结果显示,采用隔震框架结构与BRB-框架结构可以有效降低建筑物在地震作用下的维修成本与维修时间。与普通框架相比,在罕遇地震作用下隔震结构的维修成本与维修时间可降低65%与58%,BRB-支撑框架结构可降低47%与34%。

VmaH和PMA在指状青霉中的表达及其作为潜在杀菌作用靶点的可能性

目的:研发安全、高效、具有新型作用靶点的杀菌剂,在对指状青霉(Penicillium digitatum)VmaH和PMA进行生物信息学和表达模式分析的基础上,进一步探讨两个基因作为潜在杀菌作用靶点的可能性。方法:以指状青霉为实验材料,使用Pfam、MEGA 5.0等在线软件对VmaH和PMA的编码蛋白进行生物信息学分析,进一步利用实时聚合酶链式反应技术分析VmaH和PMA在指状青霉中的表达规律。结果:结构域预测发现,VmaH具有2个非常保守的结构域,分别为N端结构域和C端结构域;PMA具有3个非常保守的结构域,分别为N端结构域、E1-E2 ATPase结构域和水解酶结构域。同源比对结果发现真菌物种中VmaH和PMA的氨基酸相似度分别为79%、80%,表明不同真菌间VmaH和PMA高度保守。进化树分析显示指状青霉VmaH和PMA与青霉属和曲霉属的VmaH和PMA具有较近的亲缘关系。相较于PMA,VmaH在指状青霉的整个生长过程中一直保持较高的表达水平,而PMA的基因表达水平在指状青霉生长后期显著下调。葡萄糖饥饿显著降低了VmaH和PMA的基因表达水平,葡萄糖回补可以恢复两个基因的表达水平,尤其是VmaH的表达量会显著上调。极酸极碱条件下VmaH和PMA的表达量均显著下调,弱酸和弱碱性环境使PMA基因的表达量增加。氧化、还原剂处理均能显著降低VmaH和PMA的表达水平,且PMA的下调尤为显著。PMA在不同杀菌剂处理条件下表达量均显著下降;而VmaH对不同杀菌剂处理的响应有所差异。结论:通过VmaH和PMA的生物信息学和表达规律分析,可以将VmaH和PMA所编码的蛋白作为指状青霉潜在杀菌作用靶点进行深入研究。

Genome-wide comparative analysis of type-A Arabidopsis response regulator genes by overexpression studies reveals their diverse roles and regulatory mechanisms in cytokinin signaling

Cytokinin is a critical growth regulator for various aspects of plant growth and development. In Arabidopsis, cytokinin signaling is mediated by a two-component system-based phosphorelay that transmits a signal from the receptors, through histidine phosphotransfer proteins, to the downstream response regulators （ARRs）. Of these ARRs, type-A ARR genes, whose transcription can be rapidly induced by cytokinin, act as negative regulators of eytokinin signaling. However, because of functional redundancy, the function of type-A ARR genes in plant growth and development is not well understood by analyzing loss-of-function mutants. In this study, we performed a comparative functional study on all ten type-A ARR genes by analyzing transgenic plants overexpressing these ARR genes fused to a MYC epitope tag. Overexpression of ARR genes results in a variety of cytokinin-associated phenotypes. Notably, overexpression of different ARR transgenes causes diverse phenotypes, even between phylogenetically closely-related gene pairs, such as within the ARR3-ARR4 and ARR5-ARR6 pairs. We found that the accumulation of a subset of ARR proteins （ARR3, ARR5, ARR7, ARR16 and ARR17; possibly ARR8 and ARR15） is increased by MG132, a specific proteasomal inhibitor, indicating that stability of these proteins is regulated by proteasomal degradation. Moreover, similar to that of previously characterized ARR5, ARR6 and ARR7, stability of ARR16 and ARR17, possibly including ARR8 and ARR15, is regulated by cytokinin. These results suggest that type-A ARR proteins are regulated by a combinatorial mechanism involving both the cytokinin and proteasome pathways, thereby executing distinctive functions in plant growth and development.

Injectable hydrogels for cartilage and bone tissue engineering

Tissue engineering has become a promising strategy for repairing damaged cartilage and bone tissue. Among the scaffolds for tissue-engineering applications, injectable hydrogels have demonstrated great potential for use as three-dimensional cell culture scaffolds in cartilage and bone tissue engineering, owing to their high water content, similarity to the natural extracellular matrix(ECM), porous framework for cell transplantation and proliferation, minimal invasive properties, and ability to match irregular defects. In this review, we describe the selection of appropriate biomaterials and fabrication methods to prepare novel injectable hydrogels for cartilage and bone tissue engineering. In addition, the biology of cartilage and the bony ECM is also summarized. Finally, future perspectives for injectable hydrogels in cartilage and bone tissue engineering are discussed.

Analysis of the clinical characteristics and refraction state in premature infants: a 10-year retrospective analysis

AIM: To report the visual outcomes and refractive status in premature infants with and without retinopathy of prematurity(ROP) who were or not treated. METHODS: The clinical records of all premature infants with or without ROP and with or without treatment between 2007 and 2017 were retrospectively reviewed. Basic demographic data, serial changes in ROP incidence, treatment and outcomes, and the refractive states were analyzed. Correlations among myopia and astigmatism progression, birth weight, gestational age, and treatment methods were also analyzed.RESULTS: A total of 562 screened premature infants(all Chinese, 1124 eyes), were recruited with a 378:184 maleto-female ratio. Birth weight did not directly influence ROP incidence. The overall ROP incidence was 16.55%(93/562 cases). The incidences in boys and girls were 16.14%(33/378 cases) and 17.39%(32/184 cases), respectively, and this difference was not significant. However, all infants with serious ROP(stage IV and V) were male. Myopia combined with astigmatism was common in premature infants with and without ROP(30.99%, 172/555 cases), and myopic refraction(including myopia and myopia combined with astigmatism) was more common in premature infants with ROP(48.84%, 42/86 cases). In the >8.00 diopter group, there were significantly more ROP infants than without ROP. Myopic refraction(including myopia and myopia combined with astigmatism) was most common in infants with ROP after treatment(63.63%, 7/11 cases). CONCLUSION: The refractive state is different between premature infants and mature infants. Those treated for ROP had a higher chance of developing myopia, astigmatism, and higher diopter.

指状青霉V-ATP酶H亚基蛋白的功能

目的:通过基因干扰和过表达技术,研究V-ATP酶H亚基(V-ATPase membrane subunit H,VMAH)蛋白在柑橘绿霉病菌指状青霉生长中的生理功能,及其作为抗柑橘绿霉病药物作用靶点的可行性。方法:采用菌丝生长法和孢子萌发法,研究沉默和过表达VMAH基因对正常和胁迫条件下指状青霉细胞生长特性的影响。结果:在正常生长条件下,沉默VMAH基因显著抑制了指状青霉菌丝体的生长;而过表达VMAH基因对菌体生长表现出显著促进作用。在不同pH值环境下,沉默VMAH基因显著抑制了指状青霉菌丝的生长,尤其在偏酸性(pH值低于2)和偏碱性(pH值高于8)条件下尤为显著;而过表达VMAH基因在一定程度上促进了菌体菌丝的生长,pH值为6时,其促进作用最显著。与野生株相比,沉默VMAH基因对胞外Ca^(2+)、Cu^(2+)、Fe^(2+)等金属离子胁迫处理较为敏感。VMAH基因沉默菌株对质子泵抑制剂类杀菌剂的敏感性显著增加,尤其V-ATP酶专一性抑制剂巴佛洛霉素A_(1)最为明显;而VMAH基因过表达菌株对质子泵抑制剂类杀菌剂的敏感性显著降低。结论:VMAH在指状青霉生长过程中发挥重要作用,是研发低耐药性柑橘采后杀菌剂的潜在作用靶点。

The Arabidopsis Spontaneous Cell Death1 gene, encoding a ζ-carotene desaturase essential for carotenoid biosynthesis, is involved in chloroplast development, photoprotection and retrograde signalling

Carotenoids, a class of natural pigments found in all photosynthetic organisms, are involved in a variety of physiological processes, including coloration, photoprotection, biosynthesis of abscisic acid （ABA） and chloroplast biogenesis. Although carotenoid biosynthesis has been well studied biochemically, the genetic basis of the pathway is not well understood. Here, we report the characterization of two allelic Arabidopsis mutants, spontaneous cell death1-1 （spcl-1） and spc1-2. The weak allele spc1-1 mutant showed characteristics of bleached leaves, accumulation of superoxide and mosaic cell death. The strong mutant allele spc1-2 caused a complete arrest of plant growth and development shortly after germination, leading to a seedling-lethal phenotype. Genetic and molecular analyses indicated that SPC1 encodes a putative ζ-carotene desaturase （ZDS） in the carotenoid biosynthesis pathway. Analysis of carotenoids revealed that several major carotenoid compounds downstream of SPC 1/ZDS were substantially reduced in spc1-1, suggesting that SPC 1 is a functional ZDS. Consistent with the downregulated expression of CAO and PORB, the chlorophyll content was decreased in spc1-1 plants. In addition, expression of Lhcb1. 1, Lhcbl. 4 and RbcS was absent in spc1-2, suggesting the possible involvement of carotenoids in the plastid-to-nucleus retrograde signaling. The spc1-1 mutant also displays an ABA-deficient phenotype that can be partially rescued by the externally supplied phytohormone. These results suggest that SPC1/ZDS is essential for biosynthesis of carotenoids and plays a crucial role in plant growth and development.

Comparison of LAMP,GeneXpert,Mycobacterial Culture,Smear Microscopy,TSPOT.TB,TBAg/PHA Ratio for Diagnosis of Pulmonary Tuberculosis

Objective:To investigate the application value of loop-mediated isothermal amplification(LAMP),GeneXpert,mycobacterial culture,smear microscopy,TSPOT.TB(TSPOT),ratio of TB-specific antigen to phytohemagglutinin(TBAg/PHA ratio)in the detection of mycobacterium tuberculosis in the bronchoalveolar lavage fluid.Methods:A retrospective analysis was performed on the patients who underwent bronchoscopy from December 2018 to November 2019 in Tongji Hospital.The patients with positive tuberculosis culture or positive GeneXpert in bronchoalveolar lavage fluid were selected as the case group,and those without tuberculosis served as the control group.The receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of LAMP,GeneXpert,culture,smear microscopy,TSPOT,and TBAg/PHA ratio.Results:For the patients with positive cultures as case,the sensitivity of LAMP,GeneXpert,smear microscopy,TSPOT and TBAg/PHA ratio was 73.49%,89.16%,25.30%,80.00%,33.85%,respectively,the specificity was 99.00%,100.00%,99.00%,86.00%,100.00%,respectively,the area under the ROC curve(AUC)was 0.849,0.938,0.633,0.830,0.669,respectively.For the patients with positive GeneXpert as case,the sensitivity of LAMP,mycobacterial culture,smear microscopy,TSPOT and TBAg/PHA ratio was 73.20%,74.23%,22.68%,68.92%,29.73%,respectively,the specificity was 99.00%,100.00%,99.00%,86.00%,100.00%,respectively,the AUC was 0.853,0.878,0.623,0.775,0.649,respectively.Conclusion:The sensitivity of GeneXpert was best.The sensitivity and diagnostic value of LAMP were slightly lower than those of GeneXpert,and were similar to tuberculosis culture.The sensitivity of smear microscopy was low.The specificity of TSPOT was low.When TBAg/PHA ratio>0.2 was used as a diagnostic index,the specificity was improved,but the sensitivity was low.

Three New Indole Alkaloids from Tabernaemontana divaricata

Three new monoterpene indole alkaloids,3a-hydroxymethyl-ibogamine(1),3a-acetatemethoxyl-ibogamine(2),16a-hy-droxyl-ibogamine(3)together with six known alkaloids were isolated from the branches and leaves of Tabernaemontana divaricata(Apocynaceae).The structures of these alkaloids were determined by spectroscopic analyses.All isolated compounds showed no significant cytotoxicity against SGC-7901 gastric cancer,HeLa,and A-549 lung cancer cell lines(IC50>20μM).

Interleukin-22 regulating fibrosis on mouse cardiac fibroblasts through STAT3 signaling pathway

Objective:To observe the effects of interleukin-22(IL-22)on the expression of type Ⅲ collagen,cytokines,growth factors and chemokines in mouse cardiac fibroblasts in vitro.Methods:Mouse cardiac fibroblasts were treated with0μg/L(control),1μg/L(low concentration)and 100μg/L(high concentration)IL-22,respectively.In addition,cells treated with 100μmol/L static(an STAT3 pathway inhibitor)and 100μg/L IL-22 was defined as the block group.After treatment for 48 hours,the mRNA level of collagen type Ⅲ A1(Col3-A1),matrix metalloproteinase-1(Timp-1),IL-22receptor(IL-22R),interleukin 10-related T cellderived inducible factor beta(Iltifb),fibroblast growth factor1(Fgf1)and C-C motif chemokine ligand 4(Ccl4)were determined by RT-PCR.The expression of Col3-A1 in cardiac fibroblasts was also semi-quantified by immunofluorescence.Results:Expression of Col3-A1 decreased in the low and high concentration groups,but significantly increased in the block group(all P <0.05).The expression of Timp-1increased in the low,high concentration and block groups compared with that in the control group,but it was significantly lower in the high concentration group than that in the low concentration group(P <0.05).The expression of IL-22 Rand Iltifb was significantly increased in the low,high concentration and block groups compared with that in the control group(P <0.05),but there was no statistical difference between the high concentration group and block group.The expression of Fgf1 and Ccl4 was significantly decreased in the low,high concentration and block groups compared with that in the control group(P <0.05),but there was no statistic difference between the high concentration group and block group as well.Conclusion:IL-22 effected on the expression of Col3-A1 and Timp-1,which was possibly through the JAK-STAT3 signaling pathway in mice cardiac fibroblasts.

我国传统名贵中药材--山茱萸

唐代诗人王维曾在《山茱萸》中写道“朱实山下开,清香寒更发。”诗中所提及的“朱实”便是山茱萸。山茱萸是我国传统名贵中药材,始载于东汉时期的《神农本草经》[1]。又名蜀枣(《神农本草经》),鼠矢、鸡足(《吴普本草》),魁实、思益(《名医别录》),汤主(《药谱》),山萸肉(《小儿药证直诀》),实枣儿(《救荒本草》),肉枣(《本草纲目》),山萸(《本草经读》),枣皮(《会约医镜》)。

飞秒激光辅助超声乳化联合Ahmed青光眼引流阀植入术治疗合并难治性青光眼的白内障

目的:评估飞秒激光辅助超声乳化联合Ahmed青光眼引流阀植入术治疗合并难治性青光眼的白内障的有效性和安全性。方法:回顾性病例对照研究。2019-10/2021-10入院合并难治性青光眼的白内障患者53例53眼,依据自愿选择分为飞秒激光辅助白内障超声乳化(FLACS)组26例26眼和常规白内障超声乳化(CPCS)组27例27眼。两组分别行FLACS和CPCS联合Ahmed青光眼引流阀植入术。比较两组患者术中超声乳化能量释放量(CDE)、有效超声时间(EPT)的差异和术前与术后抗青光眼药物数量的变化,以及术后观察不同时期(1d,1wk,1、3mo)在提高最佳矫正视力(BCVA),降低眼压、角膜内皮细胞损伤程度和手术并发症及成功率状况。结果:FLACS组术中CDE和EPT明显低于CPCS组(t=8.50、5.16;P<0.01、=0.001)。两组术后抗青光眼药物较术前均明显减少(t=9.12、7.76;P=0.011、0.016),但两组间无差异(t=1.79,P=0.082)。两组术后BCVA均较术前改善,眼压均较术前降低(P<0.05)。FLACS组在术后早期(1d,1wk)BCVA的改善较CPCS组更显著(t=9.74、8.49;P=0.008、0.012),但在术后1、3mo的BCVA改善程度并无不同(t=0.62、0.44;P=1.415、2.021)。CPCS组在术后随访不同时期的角膜内皮细胞损伤较FLACS组更明显(P<0.05)。术后随访的不同时期FLACS组和CPCS组在控制眼压方面无差异(F_(组间)=0.64,P_(组间)=0.421)。FLACS组的手术并发症发生率27%(7/26)较CPCS组89%(24/27)低(χ^(2)=20.95,P<0.01),其中角膜水肿(8%vs 41%)、前囊撕裂(0 vs 11%)在FLACS组中明显低于CPCS组,后囊破裂(0 vs 7%)、玻璃体脱出(0 vs 4%)及人工晶状体偏位(0 vs 7%)也均发生在CPCS组。但两组的治疗总成功率相近(P=28.718)。结论:飞秒激光辅助超声乳化联合Ahmed青光眼引流阀植入术可充分发挥联合手术的精准微创可控优势,帮助合并难治性青光眼的白内障患者有效降低眼压及更早获得视力恢复。

Devascularization of the superior mesenteric vein without reconstruction during surgery for retroperitoneal liposarcoma: A case report and review of literature

A 61-year-old female patient with chronic hepatitis B virus infection was diagnosed with liposarcoma in a community hospital. Fine needle aspiration biopsy confirmed the diagnosis of well-differentiated liposarcoma. Abdominal computed tomographic angiography(CTA) showed that the mass adhered to and constricted the main trunk and branch of the superior mesenteric vein(SMV), especially the ileocolic vein, and collateral circulation was observed during the vascular reconstruction scan. The abdominal liposarcoma was resected. Because of the collateral circulation, devascularization of the SMV was attempted, and we resected the eroded SMV. The condition of the blood vessels was evaluated 20 d after surgery using CTA, which showed that the SMV had disappeared. Significant improvements in SMV collateral circulation and the inferior mesenteric vein were observed after vascular reconstruction. The patient had an uneventful postoperative course except for transient gastroplegia. Twenty months after surgery, the patient had a recurrence of liposarcoma. She underwent tumor resection to remove the distal small intestine and right hemicolon. We learned that(1) direct devascularization of the main SMV trunkwithout a vein graft is possible. The presence of collateralcirculation can increase the success rate of patientsundergoing radical surgery and prevent the occurrence ofserious postoperative complications. In addition,(2) thiscase demonstrated the clinical value of 3 D reconstruction.