In this research,an atmospheric-pressure air plasma is used to inactivate the multidrug-resistant Acinetobacter baumannii in liquid.The efficacy of the air plasma on bacterial deactivation and the cytobiological varia...In this research,an atmospheric-pressure air plasma is used to inactivate the multidrug-resistant Acinetobacter baumannii in liquid.The efficacy of the air plasma on bacterial deactivation and the cytobiological variations after the plasma treatment are investigated.According to colony forming units,nearly all the bacteria（6-log） are inactivated after 10 min of air plasma treatment.However,7% of the bacteria enter a viable but non-culturable state detected by the resazurin based assay during the same period of plasma exposure.Meanwhile,86% of the bacteria lose their membrane integrity in the light of SYTO 9/PI staining assay.The morphological changes in the cells are examined by scanning electron microscopy and bacteria with morphological changes are rare after plasma exposure in the liquid.The concentrations of the long-living RS,such as H2O2,NO3^- and O3,in liquid induced by plasma treatment are measured,and they increase with plasma treatment time.The changes of the intracellular ROS may be related to cell death,which may be attributed to oxidative stress and other damage effects induced by RS plasma generated in liquid.The rapid and effective bacteria inactivation may stem from the RS in the liquid generated by plasma and air plasmas may become a valuable therapy in the treatment of infected wounds.展开更多
Sulfamethoxazole(SMX)is an antibiotic and widely present in aquatic environments,so it presents a serious threat to human health and sustainable development.A dielectric barrier discharge(DBD)plasma jet was utilized t...Sulfamethoxazole(SMX)is an antibiotic and widely present in aquatic environments,so it presents a serious threat to human health and sustainable development.A dielectric barrier discharge(DBD)plasma jet was utilized to degrade aqueous SMX,and the effects of various operating parameters(working gas,discharge power,etc)on SMX degradation performance were studied.The experimental results showed that the DBD plasma jet can obtain a relatively high degradation efficiency for SMX when the discharge power is high with an oxygen atmosphere,the initial concentration of SMX is low,and the aqueous solution is under acidic conditions.The reactive species produced in the liquid phase were detected,and OH radicals and O3were found to play a significant role in the degradation of SMX.Moreover,the process of SMX degradation could be better fitted by the quasi-first-order reaction kinetic equation.The analysis of the SMX degradation process indicated that SMX was gradually decomposed and 4-amino benzene sulfonic acid,benzene sulfonamide,4-nitro SMX,and phenylsulfinyl acid were detected,and thus three possible degradation pathways were finally proposed.The mineralization degree of SMX reached 90.04%after plasma treatment for 20 min,and the toxicity of the solution fluctuated with the discharge time but eventually decreased.展开更多
目的测定临床常用30种静脉用药序贯输注过程中组间冲管液的安全剂量,为临床护理操作提供参考。方法选取临床上常用且能采用紫外分光光度仪测定药物吸光度的30种静脉用药作为实验对象,按照药品使用说明书将其配制成临床常用成品输液,模...目的测定临床常用30种静脉用药序贯输注过程中组间冲管液的安全剂量,为临床护理操作提供参考。方法选取临床上常用且能采用紫外分光光度仪测定药物吸光度的30种静脉用药作为实验对象,按照药品使用说明书将其配制成临床常用成品输液,模拟体外临床输注。输液结束时,更换与溶剂一致的0.9%氯化钠或者5%葡萄糖注射液作为冲管液,依次留取样本1 mL,共留取70个样本。利用紫外分光光度仪测定不同阶段冲管液量标本的吸光度,根据标准曲线计算浓度,当冲管液量样本浓度为0时,判定输液管内残留药液已被冲洗干净。结果30种静脉用药的冲管液体积24~65 mL,其中24~<30 mL 12种,30~<40 mL 10种,40~<50 mL 6种,50 mL 1种,65 mL 1种。22种(73.33%)药品组间冲管安全剂量在40 mL以内。结论多种静脉用药序贯输液时,当有配伍禁忌或无法确定有无配伍禁忌时,均需要使用安全剂量行组间冲管,以利于临床静脉用药安全。展开更多
Objective The effect of dietary restriction, intense exercise and menstrual dysfunction on bone mineral density remains controversial. The aim of this study was to assess the skeletal health status and relationship be...Objective The effect of dietary restriction, intense exercise and menstrual dysfunction on bone mineral density remains controversial. The aim of this study was to assess the skeletal health status and relationship between bone mineral density and nutrient intake, menstrual status, estrogen level and other factos in Chinese adolescent dancers. Methods Sixty dancers and 77 healthy controls underwent measurements of bone density, body composition, and estrogen level. Nutrient intake, menstrual status and physical activity were assessed with questionnaires. The correlation between these factors were analyzed. Results The dancers under study had a significantly lean body mass index （18.3±1.4 kg/m^2 vs. 21.7±3.1 kg/m^2）, lower percentage of body fat （0.25±0.05 vs. 0.34±0.04） and later age at menarche （14.0±0.9 y vs. 13.0±1.3 y）, and the estrogen level, daily calorie and fat intake in them were also lower than in the controls. All the dancers undertook intensive physical activity every day and up to 69% of them suffered from irregular menarche. Yet they had relatively high BMD and BMC of the total body and legs than the controls after adjusting for BM1 and age. Site-specific BMD was positively correlated to BMI, body composition and training hours per week and negatively correlated to the age at menarche and menstrual frequency. Conclusions The relatively high BMD and BMC of the dancers at the total body and legs were probably caused by high levels of weight-bearing physical activity. To ameliorate disordered eating, especially low energy intake might be helpful to prevent the Triad and to improve the bone health in adolescent dancers.展开更多
Atmospheric pressure cold plasma, with advantages such as high particle activity, no thermal damage, high efficiency and direct and friendly contact with human tissues, is considered to have great potential in biomedi...Atmospheric pressure cold plasma, with advantages such as high particle activity, no thermal damage, high efficiency and direct and friendly contact with human tissues, is considered to have great potential in biomedical applications. Therefore, 'plasma medicine' as a new interdiscipline has been developed in the past two decades. This review first briefly describes the development of typical plasma sources suitable for biomedical applications, and those with different discharge forms are simply compared, evaluated and summarized. Subsequently, measurement of the crucial gaseous reactive particles(e.g. OH and O) and their spatio-temporal distributions are introduced. Meanwhile, the generation and variation rules and the related critical macroscopic parameters of the plasma-induced aqueous reactive species are summarized. Finally, related studies in the last ten years on the mechanisms of the plasma-driven microbial inactivation and plasma-induced apoptosis of cancer cells are introduced. Moreover, some scientific problems that need to be urgently solved in the field of plasma medicine are also discussed. This review will provide useful guidance for future related research.展开更多
Tetracycline(TC)is an antibiotic mainly used in livestock production and respiratory infection.Traditional methods are not effective in removing TC from solution.In this study,TC was degraded by gas–liquid plasma in ...Tetracycline(TC)is an antibiotic mainly used in livestock production and respiratory infection.Traditional methods are not effective in removing TC from solution.In this study,TC was degraded by gas–liquid plasma in the presence of rGO-TiO_(2)in solution.The rGO-TiO_(2)was prepared by modified hummers and hydrothermal method.The electrical and optical properties of the gas–liquid discharge plasma were studied and the produced long-lived reactive species were analyzed by spectrophotometer.The degradation efficiency of TC was improved by 41.4%after plasma treatment for 12 min in presence of 30 mg l-1 r GO-TiO_(2)compared to that with plasma alone.The degradation efficiency increased with increasing discharge power,but as the initial concentration was increased from 20 to 80 mg l-1,the degradation efficiency of TC decreased.The initial p H had no significant effect on the degradation of TC.The intermediate products were determined by UV–vis spectrophotometry and ESI(+)–MS,and the degradation mechanism was analyzed.The reactive species,including O_(3),·OH,and H_(2)O_(2),etc.,produced in the plasma/catalyst system attracted electron-rich functional groups(amino group,aromatic ring,and double bond).Therefore,the gas–liquid plasma/catalyst system could be an effective and promising method for pharmaceutical wastewater treatment in future.展开更多
基金supported by the Spark Program of the second Affiliated Hospital of Anhui Medical University (Grant No.2015hhjh04)National Natural Science Foundation of China under Grant No.51777206+6 种基金Natural Science Foundation of Anhui Province (Grant No.1708085MA13 and No.1708085MB47)Science Foundation of Institute of Plasma Physics,Chinese Academy of Sciences under Grant (No.DSJJ-14-YY02)City University of Hong Kong Applied Research Grant (ARG) (No.9667144)Hong Kong Research Grants Council (RGC) General Research Funds (GRF) (No.City U 11301215)Doctoral Fund of Ministry of Education of China (No.2017M612058)Specialized Research Fund for the Doctoral Program of Hefei University of Technology (No.JZ2016HGBZ0768)Foundation of Anhui Province Key Laboratory of Medical Physics and Technology (Grant No.LMPT2017Y7BP0U1581)
文摘In this research,an atmospheric-pressure air plasma is used to inactivate the multidrug-resistant Acinetobacter baumannii in liquid.The efficacy of the air plasma on bacterial deactivation and the cytobiological variations after the plasma treatment are investigated.According to colony forming units,nearly all the bacteria（6-log） are inactivated after 10 min of air plasma treatment.However,7% of the bacteria enter a viable but non-culturable state detected by the resazurin based assay during the same period of plasma exposure.Meanwhile,86% of the bacteria lose their membrane integrity in the light of SYTO 9/PI staining assay.The morphological changes in the cells are examined by scanning electron microscopy and bacteria with morphological changes are rare after plasma exposure in the liquid.The concentrations of the long-living RS,such as H2O2,NO3^- and O3,in liquid induced by plasma treatment are measured,and they increase with plasma treatment time.The changes of the intracellular ROS may be related to cell death,which may be attributed to oxidative stress and other damage effects induced by RS plasma generated in liquid.The rapid and effective bacteria inactivation may stem from the RS in the liquid generated by plasma and air plasmas may become a valuable therapy in the treatment of infected wounds.
基金supported jointly by National Natural Science Foundation of China(Nos.U20A20372,51807046,51777206)the Natural Science Foundation of Anhui Province(Nos.2108085MD136,1908085MA29)。
文摘Sulfamethoxazole(SMX)is an antibiotic and widely present in aquatic environments,so it presents a serious threat to human health and sustainable development.A dielectric barrier discharge(DBD)plasma jet was utilized to degrade aqueous SMX,and the effects of various operating parameters(working gas,discharge power,etc)on SMX degradation performance were studied.The experimental results showed that the DBD plasma jet can obtain a relatively high degradation efficiency for SMX when the discharge power is high with an oxygen atmosphere,the initial concentration of SMX is low,and the aqueous solution is under acidic conditions.The reactive species produced in the liquid phase were detected,and OH radicals and O3were found to play a significant role in the degradation of SMX.Moreover,the process of SMX degradation could be better fitted by the quasi-first-order reaction kinetic equation.The analysis of the SMX degradation process indicated that SMX was gradually decomposed and 4-amino benzene sulfonic acid,benzene sulfonamide,4-nitro SMX,and phenylsulfinyl acid were detected,and thus three possible degradation pathways were finally proposed.The mineralization degree of SMX reached 90.04%after plasma treatment for 20 min,and the toxicity of the solution fluctuated with the discharge time but eventually decreased.
文摘目的测定临床常用30种静脉用药序贯输注过程中组间冲管液的安全剂量,为临床护理操作提供参考。方法选取临床上常用且能采用紫外分光光度仪测定药物吸光度的30种静脉用药作为实验对象,按照药品使用说明书将其配制成临床常用成品输液,模拟体外临床输注。输液结束时,更换与溶剂一致的0.9%氯化钠或者5%葡萄糖注射液作为冲管液,依次留取样本1 mL,共留取70个样本。利用紫外分光光度仪测定不同阶段冲管液量标本的吸光度,根据标准曲线计算浓度,当冲管液量样本浓度为0时,判定输液管内残留药液已被冲洗干净。结果30种静脉用药的冲管液体积24~65 mL,其中24~<30 mL 12种,30~<40 mL 10种,40~<50 mL 6种,50 mL 1种,65 mL 1种。22种(73.33%)药品组间冲管安全剂量在40 mL以内。结论多种静脉用药序贯输液时,当有配伍禁忌或无法确定有无配伍禁忌时,均需要使用安全剂量行组间冲管,以利于临床静脉用药安全。
基金supported by key projects of the national science & technology pillar program during the eleventh five-year plan period (2008BAI58B02)danone institute China diet nutrition & comunication grant proposal 2006(DIC 2006-4)
文摘Objective The effect of dietary restriction, intense exercise and menstrual dysfunction on bone mineral density remains controversial. The aim of this study was to assess the skeletal health status and relationship between bone mineral density and nutrient intake, menstrual status, estrogen level and other factos in Chinese adolescent dancers. Methods Sixty dancers and 77 healthy controls underwent measurements of bone density, body composition, and estrogen level. Nutrient intake, menstrual status and physical activity were assessed with questionnaires. The correlation between these factors were analyzed. Results The dancers under study had a significantly lean body mass index （18.3±1.4 kg/m^2 vs. 21.7±3.1 kg/m^2）, lower percentage of body fat （0.25±0.05 vs. 0.34±0.04） and later age at menarche （14.0±0.9 y vs. 13.0±1.3 y）, and the estrogen level, daily calorie and fat intake in them were also lower than in the controls. All the dancers undertook intensive physical activity every day and up to 69% of them suffered from irregular menarche. Yet they had relatively high BMD and BMC of the total body and legs than the controls after adjusting for BM1 and age. Site-specific BMD was positively correlated to BMI, body composition and training hours per week and negatively correlated to the age at menarche and menstrual frequency. Conclusions The relatively high BMD and BMC of the dancers at the total body and legs were probably caused by high levels of weight-bearing physical activity. To ameliorate disordered eating, especially low energy intake might be helpful to prevent the Triad and to improve the bone health in adolescent dancers.
基金financially supported by National Natural Science Foundation of China (Grant Nos. 51777206, 51807046 and 51877208)the Natural Science Foundation of Anhui Province (Grant Nos. 1908085MA29 and 1808085MA13)。
文摘Atmospheric pressure cold plasma, with advantages such as high particle activity, no thermal damage, high efficiency and direct and friendly contact with human tissues, is considered to have great potential in biomedical applications. Therefore, 'plasma medicine' as a new interdiscipline has been developed in the past two decades. This review first briefly describes the development of typical plasma sources suitable for biomedical applications, and those with different discharge forms are simply compared, evaluated and summarized. Subsequently, measurement of the crucial gaseous reactive particles(e.g. OH and O) and their spatio-temporal distributions are introduced. Meanwhile, the generation and variation rules and the related critical macroscopic parameters of the plasma-induced aqueous reactive species are summarized. Finally, related studies in the last ten years on the mechanisms of the plasma-driven microbial inactivation and plasma-induced apoptosis of cancer cells are introduced. Moreover, some scientific problems that need to be urgently solved in the field of plasma medicine are also discussed. This review will provide useful guidance for future related research.
基金financially supported by National Natural Science Foundation of China(Nos.51777206 and 51541807)Natural Science Foundation of Anhui Province(Nos.1908085MA29,1708085MB47 and 1708085MA13)+4 种基金Doctoral Fund of Ministry of Education of China(No.2017M612058)Specialized Research Fund for the Doctoral Program of Hefei University of Technology(No.JZ2016HGBZ0769)Chinese Academy of Sciences under Grant No.DSJJ-14-YY02Science and Technology Cooperation Program between China and Finland(No.2017YFE0115200)Hong Kong Research Grants Council(RGC)General Research Funds(GRF)(No.City U 11205617)。
文摘Tetracycline(TC)is an antibiotic mainly used in livestock production and respiratory infection.Traditional methods are not effective in removing TC from solution.In this study,TC was degraded by gas–liquid plasma in the presence of rGO-TiO_(2)in solution.The rGO-TiO_(2)was prepared by modified hummers and hydrothermal method.The electrical and optical properties of the gas–liquid discharge plasma were studied and the produced long-lived reactive species were analyzed by spectrophotometer.The degradation efficiency of TC was improved by 41.4%after plasma treatment for 12 min in presence of 30 mg l-1 r GO-TiO_(2)compared to that with plasma alone.The degradation efficiency increased with increasing discharge power,but as the initial concentration was increased from 20 to 80 mg l-1,the degradation efficiency of TC decreased.The initial p H had no significant effect on the degradation of TC.The intermediate products were determined by UV–vis spectrophotometry and ESI(+)–MS,and the degradation mechanism was analyzed.The reactive species,including O_(3),·OH,and H_(2)O_(2),etc.,produced in the plasma/catalyst system attracted electron-rich functional groups(amino group,aromatic ring,and double bond).Therefore,the gas–liquid plasma/catalyst system could be an effective and promising method for pharmaceutical wastewater treatment in future.
