[ Objective ] The purpose was to understand species and occurrence status of major fugal diseases in sugarcane cultivation areas of Guangxi in China, so as to provide scientific references for integrated control again...[ Objective ] The purpose was to understand species and occurrence status of major fugal diseases in sugarcane cultivation areas of Guangxi in China, so as to provide scientific references for integrated control against sugarcane diseases. [ Method] Species and occurrence degrees of major fugal diseases in sugarcane cultivation areas of Guangxi were investigated through randomized surveys, farmer interviews, literature references and location investigations from 2009 to 2011. Diseased sugarcane samples were collected and their species were identified through pathogen isolated culture and other relevant methods. [ Result] Thirteen spe- cies of fungal diseases were identified in the survey, including pineapple disease [ Thielaviopsis paradoxa （ de Seynes ） V. Hohnel ], smut （ Ustilago scitaminea Sydow）, top rot disease （Fusarium moniliforme Sheldon）, rust （Puccinia melanocephala H. ＆ P. Syd. ）, leaf scald disease （Stagonospora sacchari Lo et Ling）, red rot disease （Colletotrichumfalcatum Went）, zonate spot disease （Phyllosticta saccharicola P. Henn. ）, leaf red spot disease [ Pellicularia sasakii （Shirai） Ito], white rash disease （Elsinoe sacchari L. ）, eye spot [Helminthosporium sacchari （Breda de Haan） Butler], yellow spot [Mycovellosiella koepkei （kruger） Deighton], brown stripe [Bipolaris stenospilum （Drechs.） Shoem. ] and brown spot （Cercospora longipes Butler）. Smut, rust, top rot, and pineapple disease were the most rampantly detrimental to quality and yield of sugarcane. [ Conclusion] Smut is the most serious sugarcane disease; it is urgent to breed sugarcane varieties with high resistance and excellent agronomic characters to replace current sugarcane varieties susceptible to diseases.展开更多
The present PCR assay was conducted to develop rapid and sensitive detection of Phytophthora colocasiae,in order to provide a robust and reliable tool for healthy seedling production of taro and limiting the transmiss...The present PCR assay was conducted to develop rapid and sensitive detection of Phytophthora colocasiae,in order to provide a robust and reliable tool for healthy seedling production of taro and limiting the transmission and spread of the causal organism of taro leaf blight in taro planting regions.The samples were used to extract total DNA and to be detected by PCR with P.colocasiae specific primer pairs PCSP-RL F/PCSP-RL R and PCSP-T F/PCSP-T R,respectively.Distinct fragments of about 200 bp and 240 bp were amplified by PCR using primers PCSP-RL F/PCSP-RL R and PCSP-T F/PCSP-T R,respectively.The analysis of the nucleotide sequence of the PCR products were found to be 99% identical to sequence of RAS-related protein (Ypt1) and phospho-ribosylanthranilate isomerase (TRP1) in P.colocasiae,respectively.It is concluded that rapid and sensitive developed PCR assay for detection of P.colocasiae could be used in routine diagnosis and aid in management practices to mitigate taro leaf blight.展开更多
基金Supported by Science and Technology Development Fund of Guangxi Academy of Agriculture (201005)Guangxi Innovation Team of Special Project for Construction of Modern Agricultural Technology System (Sugarcane)(NYCYTXGXCXTD-02)Capacity-building of Guangxi Sugarcane Engineering and Technology Research Center (GKN 1131B01)
文摘[ Objective ] The purpose was to understand species and occurrence status of major fugal diseases in sugarcane cultivation areas of Guangxi in China, so as to provide scientific references for integrated control against sugarcane diseases. [ Method] Species and occurrence degrees of major fugal diseases in sugarcane cultivation areas of Guangxi were investigated through randomized surveys, farmer interviews, literature references and location investigations from 2009 to 2011. Diseased sugarcane samples were collected and their species were identified through pathogen isolated culture and other relevant methods. [ Result] Thirteen spe- cies of fungal diseases were identified in the survey, including pineapple disease [ Thielaviopsis paradoxa （ de Seynes ） V. Hohnel ], smut （ Ustilago scitaminea Sydow）, top rot disease （Fusarium moniliforme Sheldon）, rust （Puccinia melanocephala H. ＆ P. Syd. ）, leaf scald disease （Stagonospora sacchari Lo et Ling）, red rot disease （Colletotrichumfalcatum Went）, zonate spot disease （Phyllosticta saccharicola P. Henn. ）, leaf red spot disease [ Pellicularia sasakii （Shirai） Ito], white rash disease （Elsinoe sacchari L. ）, eye spot [Helminthosporium sacchari （Breda de Haan） Butler], yellow spot [Mycovellosiella koepkei （kruger） Deighton], brown stripe [Bipolaris stenospilum （Drechs.） Shoem. ] and brown spot （Cercospora longipes Butler）. Smut, rust, top rot, and pineapple disease were the most rampantly detrimental to quality and yield of sugarcane. [ Conclusion] Smut is the most serious sugarcane disease; it is urgent to breed sugarcane varieties with high resistance and excellent agronomic characters to replace current sugarcane varieties susceptible to diseases.
基金Sponsored by Science and Technology Major Project of Guangxi(AA17204026)Basic Research Special Fund,and Scientific and Technological Fund of Guangxi Academy of Agricultural Sciences(2016YM20,2018JZ37)Guangxi Natural Science Fund(2016GXNSFAA380195)
文摘The present PCR assay was conducted to develop rapid and sensitive detection of Phytophthora colocasiae,in order to provide a robust and reliable tool for healthy seedling production of taro and limiting the transmission and spread of the causal organism of taro leaf blight in taro planting regions.The samples were used to extract total DNA and to be detected by PCR with P.colocasiae specific primer pairs PCSP-RL F/PCSP-RL R and PCSP-T F/PCSP-T R,respectively.Distinct fragments of about 200 bp and 240 bp were amplified by PCR using primers PCSP-RL F/PCSP-RL R and PCSP-T F/PCSP-T R,respectively.The analysis of the nucleotide sequence of the PCR products were found to be 99% identical to sequence of RAS-related protein (Ypt1) and phospho-ribosylanthranilate isomerase (TRP1) in P.colocasiae,respectively.It is concluded that rapid and sensitive developed PCR assay for detection of P.colocasiae could be used in routine diagnosis and aid in management practices to mitigate taro leaf blight.
