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一种甘露糖醛酸C-5差向异构酶的Loop区域结构对酶活性的影响 被引量:1
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作者 孙明 李唐 +2 位作者 赵小明 阎圣刚 尹恒 《生物学杂志》 CAS CSCD 北大核心 2021年第2期32-35,共4页
利用大肠杆菌(Escherichia coli)表达系统,成功异源表达并纯化了来自Pseudomonas syringae的一种周质甘糖醛酸C-5差向异构酶AlgG_PSEAM及其loop^(439-455)截短体。通过对产物进行分析发现,截去loop^(439-455)后,该甘露糖醛酸C-5差向异... 利用大肠杆菌(Escherichia coli)表达系统,成功异源表达并纯化了来自Pseudomonas syringae的一种周质甘糖醛酸C-5差向异构酶AlgG_PSEAM及其loop^(439-455)截短体。通过对产物进行分析发现,截去loop^(439-455)后,该甘露糖醛酸C-5差向异构酶的活性提高了14.7%。进一步分析,发现AlgG_PSEAM同时具有褐藻多糖裂解酶活性且通过测定其裂解酶活性发现,截去loop^(439-455)后,裂解酶活性提高了23.3%。截去loop^(439-455),可同时提高AlgG_PSEAM的甘露糖醛酸C-5差向异构酶和褐藻多糖裂解酶活性。 展开更多
关键词 LOOP 异源表达 甘露糖醛酸C-5差向异构酶 褐藻多糖裂解酶
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The space-time coupling statistical model of reverberation from moving platforms 被引量:2
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作者 LIU Jianguo ZHU Lingxiao yan shenggang 《Chinese Journal of Acoustics》 CSCD 2019年第3期273-294,共22页
The space-time coupling characteristic of reverberation is one of key problems in the range of reverberation suppression with space-time processing methods.Traditional methods are not based on statistical characterist... The space-time coupling characteristic of reverberation is one of key problems in the range of reverberation suppression with space-time processing methods.Traditional methods are not based on statistical characteristics of reverberation and not suitable to analyze the space-time coupling characteristics of reverberation in dealing with problems of reverberation extensions in the actual sea environment and non-ideal arrays.The space-time coupling relationship of moving platforms is deduced and then its space-time coupling statistical model is presented.The model describes the space-time coupling law of reverberation under the conditions as complex acoustic reverberation environments and an arbitrarily specified array.The mathematical expressions of space-time coupling relationship are described when the arrays have ideal narrow beams.The space-time distribution laws of reverberation from moving platforms are shown clearly by the analytical results and computer simulations,which validates the statistical model above.The space-time distribution law of reverberation from an uniform linear array(ULA),which is a non-ideal array,is also calculated by numerical methods.It is shown that the space-time distribution law of reverberation from any actual array can be acquired with the statistical model,and to support theoretically for reverberation suppression algorithms and systems design based on space-time processing. 展开更多
关键词 DOPPLER The SPACE-TIME COUPLING STATISTICAL model of REVERBERATION from moving PLATFORMS
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