Objective To establish gas chromatography-mass spectrometry(GC-MS)fingerprint method for the petroleum ether fraction of Shenqi Jiangtang Granules(SQJTG)and evaluate the product quality.Methods The GC-MS fingerprint o...Objective To establish gas chromatography-mass spectrometry(GC-MS)fingerprint method for the petroleum ether fraction of Shenqi Jiangtang Granules(SQJTG)and evaluate the product quality.Methods The GC-MS fingerprint of petroleum ether fraction of SQJTG was established by GC-MS,and the chemical components corresponding to the fingerprint peaks were structurally identified on NIST2014.The batch consistency of SQJTG products was evaluated based on the chemical composition of petroleum ether parts by using fingerprint similarity evaluation and Principal components analysis(PCA)technology.At the same time,Hotelling's T2 and DMODX statistics are used to set the control range for the quality of different batches of products.Results Twenty-two components were identified from the petroleum ether part of SQJTG,accounting for 60.94%of the total components separated.The similarity of fingerprints of petroleum ether parts of 24 batches of SQJTG was greater than 0.95.The PCA of 24 batches of samples were all under the control limits of Hotellin’s T2 and DMODX statistics,indicating that the petroleum ether parts of different batches of SQJTG were consistent.Conclusion The developed GC-MS fingerprint method can be used to evaluate the quality of SQJTG.展开更多
2’G岩藻糖基乳糖(2’Gfucosyllactose,2’GFL)是母乳中含量最高的一种低聚糖,不能被机体直接消化,但能够被肠道菌群利用,进而影响肠道健康.目前美国食品药品监督管理局(U.S.Foodand Drug Administration,FDA)和欧洲食品安全局均已批准2...2’G岩藻糖基乳糖(2’Gfucosyllactose,2’GFL)是母乳中含量最高的一种低聚糖,不能被机体直接消化,但能够被肠道菌群利用,进而影响肠道健康.目前美国食品药品监督管理局(U.S.Foodand Drug Administration,FDA)和欧洲食品安全局均已批准2’GFL可作为婴幼儿配方乳粉和普通食品的添加成分,但在国内尚未获得相应许可.本文从肠道菌群、肠道屏障、肠道免疫系统角度综述了近年来2’GFL对肠道健康影响的研究进展.2’GFL对肠道菌群具有显著调节作用,尤其是能够特异性促进部分双歧杆菌(Bifidobacterium)菌种的增殖;同时可通过促进肠上皮细胞分化和紧密连接蛋白表达来保护肠道屏障的完整性;此外,动物实验和临床研究均发现2’GFL具有维持肠道免疫稳态和促进免疫成熟的功效.展开更多
基金We thank for the funding support from the National Key Research and Development Program of China(No.2019YFC1711200).
文摘Objective To establish gas chromatography-mass spectrometry(GC-MS)fingerprint method for the petroleum ether fraction of Shenqi Jiangtang Granules(SQJTG)and evaluate the product quality.Methods The GC-MS fingerprint of petroleum ether fraction of SQJTG was established by GC-MS,and the chemical components corresponding to the fingerprint peaks were structurally identified on NIST2014.The batch consistency of SQJTG products was evaluated based on the chemical composition of petroleum ether parts by using fingerprint similarity evaluation and Principal components analysis(PCA)technology.At the same time,Hotelling's T2 and DMODX statistics are used to set the control range for the quality of different batches of products.Results Twenty-two components were identified from the petroleum ether part of SQJTG,accounting for 60.94%of the total components separated.The similarity of fingerprints of petroleum ether parts of 24 batches of SQJTG was greater than 0.95.The PCA of 24 batches of samples were all under the control limits of Hotellin’s T2 and DMODX statistics,indicating that the petroleum ether parts of different batches of SQJTG were consistent.Conclusion The developed GC-MS fingerprint method can be used to evaluate the quality of SQJTG.
文摘2’G岩藻糖基乳糖(2’Gfucosyllactose,2’GFL)是母乳中含量最高的一种低聚糖,不能被机体直接消化,但能够被肠道菌群利用,进而影响肠道健康.目前美国食品药品监督管理局(U.S.Foodand Drug Administration,FDA)和欧洲食品安全局均已批准2’GFL可作为婴幼儿配方乳粉和普通食品的添加成分,但在国内尚未获得相应许可.本文从肠道菌群、肠道屏障、肠道免疫系统角度综述了近年来2’GFL对肠道健康影响的研究进展.2’GFL对肠道菌群具有显著调节作用,尤其是能够特异性促进部分双歧杆菌(Bifidobacterium)菌种的增殖;同时可通过促进肠上皮细胞分化和紧密连接蛋白表达来保护肠道屏障的完整性;此外,动物实验和临床研究均发现2’GFL具有维持肠道免疫稳态和促进免疫成熟的功效.
