目的:考察吗替麦考酚酯(MMF)对自身免疫性疾病患者白细胞计数和感染的影响。方法:采用自身前后对照法进行回顾性研究,纳入使用吗替麦考酚酯治疗的免疫病患者共计51人,考察治疗前后患者血液常规检查结果以及感染发生情况。结果:使用吗替...目的:考察吗替麦考酚酯(MMF)对自身免疫性疾病患者白细胞计数和感染的影响。方法:采用自身前后对照法进行回顾性研究,纳入使用吗替麦考酚酯治疗的免疫病患者共计51人,考察治疗前后患者血液常规检查结果以及感染发生情况。结果:使用吗替麦考酚酯后患者白细胞计数显著降低(6.01 vs. 4.93,P=0.007)。此外,吗替麦考酚酯的使用与细菌感染(P=0.001)和病毒感染(P=0.041)显著相关,但与真菌感染无关(P=0.050)。结论:吗替麦考酚酯治疗自身免疫性疾病可能导致白细胞减少并增加细菌感染和病毒感染发生的风险。因此,在使用吗替麦考酚酯治疗期间,建议定期复查血常规并嘱托患者注意避免劳累、受凉和感染。展开更多
In the present study, we investigated anti-inflammatory effects of Sangxingtang(SXT) on acute lung injury using a lipopolysaccharide(LPS)-induced acute lung injury(ALI) mouse model. The cell counting in the bronchoalv...In the present study, we investigated anti-inflammatory effects of Sangxingtang(SXT) on acute lung injury using a lipopolysaccharide(LPS)-induced acute lung injury(ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid(BALF) was performed. The degree of lung edema was evaluated by measuring the wet/dry weight(W/D) ratio. The superoxidase dismutase(SOD) and myeloperoxidase(MPO) activities were assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators, including tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6), were assayed by the enzyme-linked immunosorbent assay methods. Pathological changes of lung tissues were observed by Hematoxylin and eosin(HE) staining. The inflammatory signaling pathway-related proteins nuclear factor mitogen activated protein kinases(P38MAPK), extracellular regulated protein kinases(Erk), c-Jun N-terminal kinase(Jnk) and nuclear transcription factor(NF-κB) p65 expressions were measured by Western blotting. Our results showed that the treatment with the SXT markedly attenuated the inflammatory cell numbers in the BALF, decreased the levels of P-P38 MAPK, P-Erk, P-Jnk and P-NF-κB p65 and the total protein levels in lungs, improved the SOD activity and inhibited the MPO activity. Histological studies demonstrated that SXT substantially reduced the LPS-induced neutrophils in lung tissues, compared with the untreated LPS group. In conclusion, our results indicated that SXT had protective effects on LPS-induced ALI in mice.展开更多
AIM: The aim of the study was to investigate the effect and mechanism of action of synthetic salidroside in an ovalbumin(OVA)-induced asthma model in mice. METHOD: BALB/c mice were sensitized with an intraperitoneal i...AIM: The aim of the study was to investigate the effect and mechanism of action of synthetic salidroside in an ovalbumin(OVA)-induced asthma model in mice. METHOD: BALB/c mice were sensitized with an intraperitoneal injection of ovalbumin(OVA) to induce a mouse model of asthma in paracmasis. The mice were treated with dexamethasone as the positive control. At the end of the study, respiratory reactivity was detected, the numbers of various kinds of white blood cells in the bronchoalveolar lavage fluid(BALF) were counted, and the levels of IL-4 and INF-γ in BALF were determined. Quantitative PCR was used to detect the mRNA contents of IL-4 and INF-γ in lung tissue. Histologic examination was performed to observe inflammatory cellular infiltration. RESULTS: Salidroside treatment virtually eliminated airway hyper-reactivity, markedly reduced the eosinophil percent, obviously reduced the levels of IL-4 and raised INF-γ in the bronchoalveolar lavage fluid(BALF) compared with the sham-treated group. Quantitative PCR on the mRNA content of IL-4 and INF-γ provided confirmation. Lung histologic observations showed that salidroside reduced inflammation and edema. These effects were equivalent to the effects of dexamethasone. CONCLUSION: Synthetic salidroside exhibits an anti-asthma effect which is related to the regulation of Th1/Th2 balance. This provides a new possibility for treatment of allergic asthma.展开更多
文摘目的:考察吗替麦考酚酯(MMF)对自身免疫性疾病患者白细胞计数和感染的影响。方法:采用自身前后对照法进行回顾性研究,纳入使用吗替麦考酚酯治疗的免疫病患者共计51人,考察治疗前后患者血液常规检查结果以及感染发生情况。结果:使用吗替麦考酚酯后患者白细胞计数显著降低(6.01 vs. 4.93,P=0.007)。此外,吗替麦考酚酯的使用与细菌感染(P=0.001)和病毒感染(P=0.041)显著相关,但与真菌感染无关(P=0.050)。结论:吗替麦考酚酯治疗自身免疫性疾病可能导致白细胞减少并增加细菌感染和病毒感染发生的风险。因此,在使用吗替麦考酚酯治疗期间,建议定期复查血常规并嘱托患者注意避免劳累、受凉和感染。
文摘In the present study, we investigated anti-inflammatory effects of Sangxingtang(SXT) on acute lung injury using a lipopolysaccharide(LPS)-induced acute lung injury(ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid(BALF) was performed. The degree of lung edema was evaluated by measuring the wet/dry weight(W/D) ratio. The superoxidase dismutase(SOD) and myeloperoxidase(MPO) activities were assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators, including tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6), were assayed by the enzyme-linked immunosorbent assay methods. Pathological changes of lung tissues were observed by Hematoxylin and eosin(HE) staining. The inflammatory signaling pathway-related proteins nuclear factor mitogen activated protein kinases(P38MAPK), extracellular regulated protein kinases(Erk), c-Jun N-terminal kinase(Jnk) and nuclear transcription factor(NF-κB) p65 expressions were measured by Western blotting. Our results showed that the treatment with the SXT markedly attenuated the inflammatory cell numbers in the BALF, decreased the levels of P-P38 MAPK, P-Erk, P-Jnk and P-NF-κB p65 and the total protein levels in lungs, improved the SOD activity and inhibited the MPO activity. Histological studies demonstrated that SXT substantially reduced the LPS-induced neutrophils in lung tissues, compared with the untreated LPS group. In conclusion, our results indicated that SXT had protective effects on LPS-induced ALI in mice.
基金supported by major drugdiscovery projects during the 12th five year plan(No.2011ZX09102-002-01)
文摘AIM: The aim of the study was to investigate the effect and mechanism of action of synthetic salidroside in an ovalbumin(OVA)-induced asthma model in mice. METHOD: BALB/c mice were sensitized with an intraperitoneal injection of ovalbumin(OVA) to induce a mouse model of asthma in paracmasis. The mice were treated with dexamethasone as the positive control. At the end of the study, respiratory reactivity was detected, the numbers of various kinds of white blood cells in the bronchoalveolar lavage fluid(BALF) were counted, and the levels of IL-4 and INF-γ in BALF were determined. Quantitative PCR was used to detect the mRNA contents of IL-4 and INF-γ in lung tissue. Histologic examination was performed to observe inflammatory cellular infiltration. RESULTS: Salidroside treatment virtually eliminated airway hyper-reactivity, markedly reduced the eosinophil percent, obviously reduced the levels of IL-4 and raised INF-γ in the bronchoalveolar lavage fluid(BALF) compared with the sham-treated group. Quantitative PCR on the mRNA content of IL-4 and INF-γ provided confirmation. Lung histologic observations showed that salidroside reduced inflammation and edema. These effects were equivalent to the effects of dexamethasone. CONCLUSION: Synthetic salidroside exhibits an anti-asthma effect which is related to the regulation of Th1/Th2 balance. This provides a new possibility for treatment of allergic asthma.