水稻花器官数目突变体mf2的鉴定和基因定位

水稻的花器官发育影响着水稻的产量与品质。本研究通过^(12)C重离子诱变航恢7号获得一个水稻花器官突变体multi-floret 2(mf2),其稃片、浆片、雄蕊、雌蕊增多,多数小穗内具2~3朵类似小花。mf2内外稃不能很好勾合,而且形状和维管束的数目都产生了一定程度的变化。电镜扫描幼穗发现花器官的变异在幼穗分化期的各花器官原基分化时就已形成。另外,该突变体的抽穗期推迟,株高降低,穗数增多,表明其营养生长也受到一定的影响。遗传分析表明mf2突变体表型受单隐性核基因控制。利用SSR、InDel分子标记将MF2定位于第1染色体的标记SSR39108和InD39210之间,区间大小约为102 kb。

Significant association of the novel Rf4-targeted SNP marker with the restorer for WA-CMS in different rice backgrounds and its utilization in molecular screening

In the rice cytoplasmic-genetic male sterility （CMS） system, the combination of a CMS line, maintainer line and restorer line carrying the restorer gene to restore fertility, is indispensable for the development of hybrids. However, the process of screening for the trait of fertility restoration is laborious and time-consuming. In the present study, we analyzed the nucleotide sequence of the Rf4 gene, which is the major locus controlling fertility restoration, to identify allele-specific variation. A single nucleotide polymorphism （SNP） A/C at ＋474 in the coding sequence （CDS） was found to be capable of strictly distinguishing groups of alleles Rf4 （A） and rf4 （C）. Using KASP genotyping, this valuable SNP was converted to an allele-specific PCR marker. We evaluated and validated the marker among three-line parents with different backgrounds, and the results revealed a complete correlation between SNP alleles and the fertility restoration phenotype. Molecular screening was subsequently carried out for the presence of alleles of Rf4 and Rf3 among 328 diverse rice cultivars with worldwide distribution. The results demonstrate that this SNP marker could be the optimal choice for the molecular identification of potential restorers.

负荷批量控制在娄底电网中的应用

根据负荷批量控制系统在娄底电网的应用情况,基于娄底电网的实际情况,结合地调E8000系统建设,研发了适用于娄底电网的负荷批量控制系统。阐述系统总体架构和软件功能,结合试验验证了该批量负荷快速控制系统的各项功能。由于控制系统的设计先进合理,运行稳定可靠,切实提高了发生事故紧急情况下调控机构对负荷控制的精准度和时效性。