Pyramid cut blasting is an essential form of inclined hole cut blasting,but the in-situ stress effect of pyramid cut blasting is rarely studied.Based on the research background of pyramid cut blasting in a deep rock m...Pyramid cut blasting is an essential form of inclined hole cut blasting,but the in-situ stress effect of pyramid cut blasting is rarely studied.Based on the research background of pyramid cut blasting in a deep rock mass,the size,volume,and fragment size distribution of the blasting cavity before and after uniaxial compression were analysed by a model test.Otherwise,the damage and effective stress of the pyramid cut blasting were analysed with LS-DYNA numerical simulation.The results show that the damage and fragmentation of pyramid cut blasting are not only affected by blasting stress wave and blasting gas,but also affected by uniaxial compression.Under the influence of uniaxial compression,the blasting stress wave and blasting gas are more likely to damage the rock mass parallel to the uniaxial compression direction near the connecting line of blasting hole,and make the volume of cavity larger and the fragment rate lower.Additionally,uniaxial compression has a prominent influence during the middle and late stages of blasting.展开更多
文摘儿童急性T淋巴细胞白血病(T-cell acute lymphoblastic leukemia,T-ALL)是T系前体细胞发生恶性转化的一种侵袭性肿瘤,化疗药物的毒副作用及耐药性问题仍然是阻碍其治疗成功的难题。长春新碱(vincristine,VCR)是治疗儿童T-ALL疗效显著的传统化疗药物,但其副作用明显。碳酸锂(Li_(2)CO_(3))可增强其它化疗药物的疗效,但其联合VCR的研究未见报道。该研究旨在探讨Li_(2)CO_(3)联合VCR对2种儿童T-ALL细胞系CCRF-CEM和Jurkat细胞增殖、凋亡及细胞周期的作用。噻唑蓝(thiazolyl blue tetrazolium bromide,MTT)比色法结果显示,与对照组比较,单用VCR或Li_(2)CO_(3),随着其浓度的增加,2种细胞存活率均逐渐降低(P<0.05)。2种细胞在相同Li_(2)CO_(3)浓度下存活率不同(P<0.01)。Li_(2)CO_(3)联合VCR处理细胞存活率与单独VCR组处理相比,2种细胞的细胞存活率和VCR的半抑制浓度(half maximal inhibitory concentration,IC_(50))值降低,Li_(2)CO_(3)与VCR的2药相互作用指数(coefficient of drug interaction,CDI)均小于1。流式细胞仪检测结果发现,对照组、Li_(2)CO_(3)组、VCR组和Li_(2)CO_(3)联合VCR组,2种细胞Li_(2)CO_(3)联合VCR组的G_(2)/M期和凋亡细胞占比最高,Li_(2)CO_(3)联合VCR组和VCR组比,均存在显著性差异(P<0.05)。总之,我们的研究结果提示,Li_(2)CO_(3)与VCR联合促进T-ALL细胞增殖抑制,使细胞周期阻滞于G_(2)/M期,且促进细胞凋亡,结果为儿童T-ALL临床治疗及减少VCR毒副作用提供了新的实验依据,也为其药物研发提供新的思路。
基金supported by the National Natural Science Foundation of China(Grant No.51974316)the National Key R&D Program of China(Grant No.2021YFB3401500)the National Key Research and Development Program of China(Grant No.2016YFC0600903)。
文摘Pyramid cut blasting is an essential form of inclined hole cut blasting,but the in-situ stress effect of pyramid cut blasting is rarely studied.Based on the research background of pyramid cut blasting in a deep rock mass,the size,volume,and fragment size distribution of the blasting cavity before and after uniaxial compression were analysed by a model test.Otherwise,the damage and effective stress of the pyramid cut blasting were analysed with LS-DYNA numerical simulation.The results show that the damage and fragmentation of pyramid cut blasting are not only affected by blasting stress wave and blasting gas,but also affected by uniaxial compression.Under the influence of uniaxial compression,the blasting stress wave and blasting gas are more likely to damage the rock mass parallel to the uniaxial compression direction near the connecting line of blasting hole,and make the volume of cavity larger and the fragment rate lower.Additionally,uniaxial compression has a prominent influence during the middle and late stages of blasting.
