Objective:To explore the underlying mechanism of inhibition by Jingui Shenqi Pills(JGSQP)on glucocorticoid-enhanced axial length elongation in experimental lens-induced myopia(LIM)guinea pigs.Methods:Sixty 2-week old ...Objective:To explore the underlying mechanism of inhibition by Jingui Shenqi Pills(JGSQP)on glucocorticoid-enhanced axial length elongation in experimental lens-induced myopia(LIM)guinea pigs.Methods:Sixty 2-week old male guinea pigs were randomly divided into 4 groups with 15 guinea pigs in each group,according to the random numbers generated by SPSS software:control,LIM,saline and JGSQP groups.The control group includes animals with no treatment,while the guinea pigs in the other 3 groups received lens-induced myopization on the right eyes throughout the experiment(for 8 weeks).The saline and JGSQP groups were given daily intraperitoneal injections of 10 mg/kg hydrocortisone for 2 consecutive weeks at the same time,and then orally administered either saline or JGSQP 13.5 g/(kg·d)for 6 consecutive weeks.Body weight,anal temperature and animal appearance were observed and recorded to evaluate the GC-associated symptoms.The ocular parameters,including refraction and axial length,were measured by streak retinoscopy and A-scan ultrasonography,respectively.The levels of plasma hormones associated with the hypothalamic-pituitary-adrenal axis(HPAA),including free triiodothyronine,free thyroxine,estradiol and testosterone,were measured by radioimmunoassay,and cyclic adenosine monophosphate(cAMP)and cyclic guanosine monophosphate were measured by enzyme-linked immunosorbent assay.In addition,the mRNA and protein expressions of retinal amphiregulin(AREG)was measured by quantitative real-time polymerase chain reaction and Western blotting,respectively.Results:JGSQP effectively increased body weight and anal temperature,improved animal appearance and suppressed axial length elongation in glucocorticoidenhanced myopic guinea pigs with normalization of 4 HPAA-associated plasma hormones(all P<0.05).The plasma level of cAMP was significantly increased,whereas the plasma level of cGMP and the mRNA and protein expressions of retinal AREG were decreased after treatment with JGSQP(all P<0.05).Conclusion:JGSQP exhibited a significant inhibitory effect on axial length elongation with decreased expression of AREG in the retina,and normalized 4 HPAA-associated plasma hormones and the expression of cAMP and cGMP in GC-enhanced myopic guinea pigs.展开更多
基金Supported by the National Key Research and Development Program(Nos.2021YFC2702103,2019YFC1710204)the Key Research and Development Program of Shandong Province(No.2019GSF108252)the Natural Science Foundation of Shandong Province(No.ZR2020MH393)。
文摘Objective:To explore the underlying mechanism of inhibition by Jingui Shenqi Pills(JGSQP)on glucocorticoid-enhanced axial length elongation in experimental lens-induced myopia(LIM)guinea pigs.Methods:Sixty 2-week old male guinea pigs were randomly divided into 4 groups with 15 guinea pigs in each group,according to the random numbers generated by SPSS software:control,LIM,saline and JGSQP groups.The control group includes animals with no treatment,while the guinea pigs in the other 3 groups received lens-induced myopization on the right eyes throughout the experiment(for 8 weeks).The saline and JGSQP groups were given daily intraperitoneal injections of 10 mg/kg hydrocortisone for 2 consecutive weeks at the same time,and then orally administered either saline or JGSQP 13.5 g/(kg·d)for 6 consecutive weeks.Body weight,anal temperature and animal appearance were observed and recorded to evaluate the GC-associated symptoms.The ocular parameters,including refraction and axial length,were measured by streak retinoscopy and A-scan ultrasonography,respectively.The levels of plasma hormones associated with the hypothalamic-pituitary-adrenal axis(HPAA),including free triiodothyronine,free thyroxine,estradiol and testosterone,were measured by radioimmunoassay,and cyclic adenosine monophosphate(cAMP)and cyclic guanosine monophosphate were measured by enzyme-linked immunosorbent assay.In addition,the mRNA and protein expressions of retinal amphiregulin(AREG)was measured by quantitative real-time polymerase chain reaction and Western blotting,respectively.Results:JGSQP effectively increased body weight and anal temperature,improved animal appearance and suppressed axial length elongation in glucocorticoidenhanced myopic guinea pigs with normalization of 4 HPAA-associated plasma hormones(all P<0.05).The plasma level of cAMP was significantly increased,whereas the plasma level of cGMP and the mRNA and protein expressions of retinal AREG were decreased after treatment with JGSQP(all P<0.05).Conclusion:JGSQP exhibited a significant inhibitory effect on axial length elongation with decreased expression of AREG in the retina,and normalized 4 HPAA-associated plasma hormones and the expression of cAMP and cGMP in GC-enhanced myopic guinea pigs.
