目的:运用网络药理学探究虎杖治疗非酒精性脂肪性肝炎(Non-alcoholic steatohepatitis,NASH)的主要活性成分及作用机制。方法:通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology,TCMSP)获得虎杖...目的:运用网络药理学探究虎杖治疗非酒精性脂肪性肝炎(Non-alcoholic steatohepatitis,NASH)的主要活性成分及作用机制。方法:通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology,TCMSP)获得虎杖主要活性成分,于Pubmed compound数据库下载其结构式并导入Pharmmapper数据库获得成分作用靶点;借助Genecards、OMIM、Disgenet等数据库获得非酒精性脂肪性肝炎的疾病靶点;绘制药物-疾病交集靶点韦恩图,Cytoscape 3.9.1软件构建成分-靶点网络;String数据库获得PPI网络并通过拓扑分析得核心靶点网络,于Metascape数据库对潜在靶点进行GO、KEGG富集分析,微生信在线制图平台绘制气泡图及条形图等。结果:经筛选获得虎杖主要活性成分10个,主要活性成分作用靶点436个,疾病靶点3944个;去重结合得疾病靶点。虎杖抗NASH潜在作用靶点81个,6,8-Dihydroxy-7-methoxyxanthone、luteolin、Physovenine等可能为虎杖作用NASH的主要活性成分;PPI网络拓扑分析得到SRC、RXRA、GRB2、AKT1、RXRB、ESR1、STAT1、JAK2、IGF1、IGF1R等10个核心靶点;GO富集分析主要包含生物过程(Biological process,BP)条目1092个、细胞组分(Cellular component,CC)条目49个、分子功能(Molecular function,MF)条目98个,KEGG分析结果130条,涉及调节细胞对脂质的反应(Cellular response to lipid)、细胞内受体信号通路(Intracellular receptor signaling pathway)等生物过程,通过PI3K/Akt等信号通路发挥作用。结论:虎杖可通过多成分、多靶点、多通路对非酒精性脂肪性肝炎发挥治疗作用,其可能涉及抗炎、抗氧化、抗凋亡、调节代谢、抑制细胞增殖等生物活性。虎杖中的多种活性成分如6,8-Dihydroxy-7-methoxyxanthone、luteolin、Physovenine等可能通过SRC、RXRA、GRB2、AKT1等靶点对信号通路进行调控,涉及多种生物过程,从而发挥抗NASH的作用。展开更多
Objective:To explore the prevention and treatment effect of Yangyin Qingre Shengji Decoction(养阴清热生肌汤)on acute radiation esophagitis.Methods:From May 2020 to February 2021,60 patients with malignant tumor who un...Objective:To explore the prevention and treatment effect of Yangyin Qingre Shengji Decoction(养阴清热生肌汤)on acute radiation esophagitis.Methods:From May 2020 to February 2021,60 patients with malignant tumor who underwent radiotherapy were selected as the research objects.They were divided into two groups by odd and even number drawing,30 cases in the control group and 30 cases in the observation group.The control group took mixed solution orally,while the observation group took Yangyin Qingre Shengji Decoction.The clinical effects,occurrence time,duration,grading of acute radiation esophagitis and TCM syndrome scores of dry mouth and constipation were compared between the two groups.Results:The effective rate of the observation group was significantly higher than that of the control group(P<0.05);Compared with the control group,the time of acute radiation esophagitis was prolonged and the duration of symptoms was shortened in the observation group(P<0.05);The incidence of above gradeⅡin the observation group was significantly lower than that in the control group,and the incidence of below gradeⅠwas significantly higher than that in the control group(P<0.05);There was no significant difference in the scores of dry mouth and constipation between the two groups before treatment(P>0.05),the scores of the two groups were decreased after treatment,and the observation group was lower than the control group(P<0.05).Conclusion:Yangyin Qingre Shengji Decoction(养阴清热生肌汤)has a positive effect on prevention and treatment of acute radiation esophagitis in patients with malignant tumor undergoing radiotherapy,which can be popularized.展开更多
文摘目的:运用网络药理学探究虎杖治疗非酒精性脂肪性肝炎(Non-alcoholic steatohepatitis,NASH)的主要活性成分及作用机制。方法:通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology,TCMSP)获得虎杖主要活性成分,于Pubmed compound数据库下载其结构式并导入Pharmmapper数据库获得成分作用靶点;借助Genecards、OMIM、Disgenet等数据库获得非酒精性脂肪性肝炎的疾病靶点;绘制药物-疾病交集靶点韦恩图,Cytoscape 3.9.1软件构建成分-靶点网络;String数据库获得PPI网络并通过拓扑分析得核心靶点网络,于Metascape数据库对潜在靶点进行GO、KEGG富集分析,微生信在线制图平台绘制气泡图及条形图等。结果:经筛选获得虎杖主要活性成分10个,主要活性成分作用靶点436个,疾病靶点3944个;去重结合得疾病靶点。虎杖抗NASH潜在作用靶点81个,6,8-Dihydroxy-7-methoxyxanthone、luteolin、Physovenine等可能为虎杖作用NASH的主要活性成分;PPI网络拓扑分析得到SRC、RXRA、GRB2、AKT1、RXRB、ESR1、STAT1、JAK2、IGF1、IGF1R等10个核心靶点;GO富集分析主要包含生物过程(Biological process,BP)条目1092个、细胞组分(Cellular component,CC)条目49个、分子功能(Molecular function,MF)条目98个,KEGG分析结果130条,涉及调节细胞对脂质的反应(Cellular response to lipid)、细胞内受体信号通路(Intracellular receptor signaling pathway)等生物过程,通过PI3K/Akt等信号通路发挥作用。结论:虎杖可通过多成分、多靶点、多通路对非酒精性脂肪性肝炎发挥治疗作用,其可能涉及抗炎、抗氧化、抗凋亡、调节代谢、抑制细胞增殖等生物活性。虎杖中的多种活性成分如6,8-Dihydroxy-7-methoxyxanthone、luteolin、Physovenine等可能通过SRC、RXRA、GRB2、AKT1等靶点对信号通路进行调控,涉及多种生物过程,从而发挥抗NASH的作用。
文摘Objective:To explore the prevention and treatment effect of Yangyin Qingre Shengji Decoction(养阴清热生肌汤)on acute radiation esophagitis.Methods:From May 2020 to February 2021,60 patients with malignant tumor who underwent radiotherapy were selected as the research objects.They were divided into two groups by odd and even number drawing,30 cases in the control group and 30 cases in the observation group.The control group took mixed solution orally,while the observation group took Yangyin Qingre Shengji Decoction.The clinical effects,occurrence time,duration,grading of acute radiation esophagitis and TCM syndrome scores of dry mouth and constipation were compared between the two groups.Results:The effective rate of the observation group was significantly higher than that of the control group(P<0.05);Compared with the control group,the time of acute radiation esophagitis was prolonged and the duration of symptoms was shortened in the observation group(P<0.05);The incidence of above gradeⅡin the observation group was significantly lower than that in the control group,and the incidence of below gradeⅠwas significantly higher than that in the control group(P<0.05);There was no significant difference in the scores of dry mouth and constipation between the two groups before treatment(P>0.05),the scores of the two groups were decreased after treatment,and the observation group was lower than the control group(P<0.05).Conclusion:Yangyin Qingre Shengji Decoction(养阴清热生肌汤)has a positive effect on prevention and treatment of acute radiation esophagitis in patients with malignant tumor undergoing radiotherapy,which can be popularized.
