目的调查新疆维吾尔自治区农村老年人口腔健康相关生活质量(oral health-related quality of life,OHRQoL)现状,分析其影响因素,为制定有效的口腔卫生服务政策提供参考依据。方法采用老年口腔健康评估指数(geriatric oral health assess...目的调查新疆维吾尔自治区农村老年人口腔健康相关生活质量(oral health-related quality of life,OHRQoL)现状,分析其影响因素,为制定有效的口腔卫生服务政策提供参考依据。方法采用老年口腔健康评估指数(geriatric oral health assessment index inventory,GOHAI)、简明口腔健康评估表对新疆农村1093名老年人进行口腔健康状况及其相关生活质量调查,运用多元线性回归分析影响因素。结果GOHAI总分为(49.66±7.15)分,生理功能、疼痛与不适和心理社会3个维度得分分别为(15.25±3.67)、(12.35±2.28)、(22.07±3.55)分,从负面影响比例来看,生理功能维度的两个条目:“咬或咀嚼食物困难”、“所吃食物的种类和数量受限”影响最大,分别为40.53%、32.85%;独居、月收入≤1000元、蔬菜摄入<1盘/d、患慢性病数量≥1种、1年内看过牙医、口腔健康状况差的老年人OHRQoL为低水平。结论新疆农村老年人OHRQoL为中低水平;影响OHRQoL的因素是多方面的,包括居住方式、月收入、蔬菜摄入量、患慢性病数量、1年内看牙医次数、口腔健康状况,应采取综合措施进行防治。展开更多
Background Gastric cancer ranks high among the most common causes of cancer-related death worldwide. This study was designed to explore key genes involved in the progression of normal gastric epithelial cells to moder...Background Gastric cancer ranks high among the most common causes of cancer-related death worldwide. This study was designed to explore key genes involved in the progression of normal gastric epithelial cells to moderate gastric epithelial dysplasia （mGED） and to gastric cancer. Methods Twelve pairs of mGED tissues, gastric cancer tissues, and normal gastric tissues were collected by gastroscopy. Total RNA was then extracted and purified. After the addition of fluorescent tags, hybridization was carried out on a Gene chip microarray slide. Significance analysis of microarrays was performed to determine significant differences in gene expression between the different tissue types. Results Microarray data analysis revealed totally 34 genes that were expressed differently： 18 highly expressed （fold change 〉2; P 〈0.01） and 16 down-regulated （fold change 〉2; P 〈0.01）. Of the 34 genes, 24 belonged to several different functional categories such as structural molecule activity, extracellular regions, structural formation, cell death, biological adhesion, developmental processes, locomotion, and biological regulation that were associated with cancer. The remaining 10 genes were not involved in cancer research. Of these genes, the expression levels of Matrix metalloproteinase-12 （MMP12）, Caspase-associated recruitment domain 14 （CARD14）, and Chitinase 3-like 1 （CHI3L1） were confirmed by semi-quantitative RT-PCR. A two-way clustering algorithm divided the 36 samples into three categories and the overall correct classification efficiency was 80.6% （29/36）. Almost all of these genes （31/34） showed constant changes in the process of normal gastric epithelial cells to mGED to gastric cancer. Conclusions The results of this study provided global gene expression profiles during the development and progression from normal gastric epithelial cells to mGED to gastric cancer. These data may provide new insights into the molecular pathology of gastric cancer which may be useful for the detection, diagnosis, and treatment.展开更多
Background The precise molecular mechanisms underlying the gallbladder carcinoma （GBC） metastasis has not been fully elucidated. Methods In the present study, metastasis-associated proteins were identified by compar...Background The precise molecular mechanisms underlying the gallbladder carcinoma （GBC） metastasis has not been fully elucidated. Methods In the present study, metastasis-associated proteins were identified by comparative proteomic analysis. The functional study of the candidate protein vimentin was further investigated. First, a pair of higher and lower metastatic sublines （termed GBC-SD/M3 and GBC-SD, respectively）, originated from the same parental cell line, was screened by spontaneous tumorigenicity and metastasis in vivo in animal study and further characterized by metastatic phenotypes analysis in vitro. Subsequently, a proteomic approach comprised two-dimensional gel electrophoresis analysis and mass spectroscopy was used to identify and compare the protein expression patterns between higher metastatic GBC-SD/M3 and lower metastatic GBC-SD cell lines. Then twenty-six proteins were identified. Results Among the 26 proteins identified, fourteen proteins were up-regulated and 12 proteins were down-regulated in GBC-SD/M3. Vimentin was identified and found to be overexpressed in GBC-SD/M3 as compared with GBC-SD. This result was further confirmed by quantitative PCR and Western blotting analysis. Furthermore, the cell migration and invasion potency of GBC-SD/M3 in vitro was remarkably suppressed after small interference RNA-mediated knockdown of vimentin. Moreover, immunoblot and immunohistochemical analysis on 12 human GBC specimens showed consistently increased vimentin expression in metastases compared with primary tumors. Conclusion Tumor vimentin level may reflect the pathological progression in some GBC and may be a useful marker for predicting tumor metastasis and a therapeutic target for the treatment of GBC patients with metastases.展开更多
文摘目的调查新疆维吾尔自治区农村老年人口腔健康相关生活质量(oral health-related quality of life,OHRQoL)现状,分析其影响因素,为制定有效的口腔卫生服务政策提供参考依据。方法采用老年口腔健康评估指数(geriatric oral health assessment index inventory,GOHAI)、简明口腔健康评估表对新疆农村1093名老年人进行口腔健康状况及其相关生活质量调查,运用多元线性回归分析影响因素。结果GOHAI总分为(49.66±7.15)分,生理功能、疼痛与不适和心理社会3个维度得分分别为(15.25±3.67)、(12.35±2.28)、(22.07±3.55)分,从负面影响比例来看,生理功能维度的两个条目:“咬或咀嚼食物困难”、“所吃食物的种类和数量受限”影响最大,分别为40.53%、32.85%;独居、月收入≤1000元、蔬菜摄入<1盘/d、患慢性病数量≥1种、1年内看过牙医、口腔健康状况差的老年人OHRQoL为低水平。结论新疆农村老年人OHRQoL为中低水平;影响OHRQoL的因素是多方面的,包括居住方式、月收入、蔬菜摄入量、患慢性病数量、1年内看牙医次数、口腔健康状况,应采取综合措施进行防治。
文摘Background Gastric cancer ranks high among the most common causes of cancer-related death worldwide. This study was designed to explore key genes involved in the progression of normal gastric epithelial cells to moderate gastric epithelial dysplasia （mGED） and to gastric cancer. Methods Twelve pairs of mGED tissues, gastric cancer tissues, and normal gastric tissues were collected by gastroscopy. Total RNA was then extracted and purified. After the addition of fluorescent tags, hybridization was carried out on a Gene chip microarray slide. Significance analysis of microarrays was performed to determine significant differences in gene expression between the different tissue types. Results Microarray data analysis revealed totally 34 genes that were expressed differently： 18 highly expressed （fold change 〉2; P 〈0.01） and 16 down-regulated （fold change 〉2; P 〈0.01）. Of the 34 genes, 24 belonged to several different functional categories such as structural molecule activity, extracellular regions, structural formation, cell death, biological adhesion, developmental processes, locomotion, and biological regulation that were associated with cancer. The remaining 10 genes were not involved in cancer research. Of these genes, the expression levels of Matrix metalloproteinase-12 （MMP12）, Caspase-associated recruitment domain 14 （CARD14）, and Chitinase 3-like 1 （CHI3L1） were confirmed by semi-quantitative RT-PCR. A two-way clustering algorithm divided the 36 samples into three categories and the overall correct classification efficiency was 80.6% （29/36）. Almost all of these genes （31/34） showed constant changes in the process of normal gastric epithelial cells to mGED to gastric cancer. Conclusions The results of this study provided global gene expression profiles during the development and progression from normal gastric epithelial cells to mGED to gastric cancer. These data may provide new insights into the molecular pathology of gastric cancer which may be useful for the detection, diagnosis, and treatment.
基金DONG Ping and HE Xiao-wei contributed equally to this work. This study was supported by grants from the National Natural Science Foundation of China （No. 30640060, 30872502, 30972918, 81172026, 81172029）, Natural Science Foundation of Zhejiang Province for Outstanding Young Researcher Groups （No. R2080452）, Foundation of Medical School, Shanghai Jiao Tong University （No. 09XJ21012） and Natural Science Foundation of Zhejiang Province （No. Y206860）.
文摘Background The precise molecular mechanisms underlying the gallbladder carcinoma （GBC） metastasis has not been fully elucidated. Methods In the present study, metastasis-associated proteins were identified by comparative proteomic analysis. The functional study of the candidate protein vimentin was further investigated. First, a pair of higher and lower metastatic sublines （termed GBC-SD/M3 and GBC-SD, respectively）, originated from the same parental cell line, was screened by spontaneous tumorigenicity and metastasis in vivo in animal study and further characterized by metastatic phenotypes analysis in vitro. Subsequently, a proteomic approach comprised two-dimensional gel electrophoresis analysis and mass spectroscopy was used to identify and compare the protein expression patterns between higher metastatic GBC-SD/M3 and lower metastatic GBC-SD cell lines. Then twenty-six proteins were identified. Results Among the 26 proteins identified, fourteen proteins were up-regulated and 12 proteins were down-regulated in GBC-SD/M3. Vimentin was identified and found to be overexpressed in GBC-SD/M3 as compared with GBC-SD. This result was further confirmed by quantitative PCR and Western blotting analysis. Furthermore, the cell migration and invasion potency of GBC-SD/M3 in vitro was remarkably suppressed after small interference RNA-mediated knockdown of vimentin. Moreover, immunoblot and immunohistochemical analysis on 12 human GBC specimens showed consistently increased vimentin expression in metastases compared with primary tumors. Conclusion Tumor vimentin level may reflect the pathological progression in some GBC and may be a useful marker for predicting tumor metastasis and a therapeutic target for the treatment of GBC patients with metastases.
