本文借助CiteSpace软件提供的知识图谱,系统梳理Web of science和CNKI数据库中国内外关于大学生心理健康研究的期刊,通过对研究文献发文量、作者、机构和关键词的分析,对国内外研究的热点和趋势进行梳理。研究发现,抑郁症、社会支持是...本文借助CiteSpace软件提供的知识图谱,系统梳理Web of science和CNKI数据库中国内外关于大学生心理健康研究的期刊,通过对研究文献发文量、作者、机构和关键词的分析,对国内外研究的热点和趋势进行梳理。研究发现,抑郁症、社会支持是国内外大学生心理健康领域研究的共同热点;心理问题应对方式和心理健康问题是该领域研究的前沿。展开更多
Proteases in male reproductive tract are considered to play key roles in fertilization processes.In contrast to mammals,there are limited data concerning crustacean sperm proteases.We previously identified a novel spe...Proteases in male reproductive tract are considered to play key roles in fertilization processes.In contrast to mammals,there are limited data concerning crustacean sperm proteases.We previously identified a novel sperm gelatinase(MSG) from Macrobrachium rosenbergii that was inhibited by a male reproduction-related Kazal-type protease inhibitor(MRPINK) specifically.In the present study,MSG was found to be distributed on the vas deferens and terminal ampullae by Western blot.Immunohistochemical analyses revealed that MSG was expressed in secretory epithelial cells and sperm distributed mainly at the base zone.RNA interference(RNAi) mediated knock-down of MSG resulted in a marked loss of sperm gelatinolytic activity.Taken together,the results show that MSG is closely linked to and probably involved in the fertilization process.展开更多
文摘本文借助CiteSpace软件提供的知识图谱,系统梳理Web of science和CNKI数据库中国内外关于大学生心理健康研究的期刊,通过对研究文献发文量、作者、机构和关键词的分析,对国内外研究的热点和趋势进行梳理。研究发现,抑郁症、社会支持是国内外大学生心理健康领域研究的共同热点;心理问题应对方式和心理健康问题是该领域研究的前沿。
基金supported by the National Basic Research Program of China (2010CB833803)National Natural Science Foundation of China(40730212,30901093)Zhejiang Marine Biotechnology Innovation Team (2010R50029,2012R10029-14)
文摘Proteases in male reproductive tract are considered to play key roles in fertilization processes.In contrast to mammals,there are limited data concerning crustacean sperm proteases.We previously identified a novel sperm gelatinase(MSG) from Macrobrachium rosenbergii that was inhibited by a male reproduction-related Kazal-type protease inhibitor(MRPINK) specifically.In the present study,MSG was found to be distributed on the vas deferens and terminal ampullae by Western blot.Immunohistochemical analyses revealed that MSG was expressed in secretory epithelial cells and sperm distributed mainly at the base zone.RNA interference(RNAi) mediated knock-down of MSG resulted in a marked loss of sperm gelatinolytic activity.Taken together,the results show that MSG is closely linked to and probably involved in the fertilization process.
