Objective:To explore the effects of N6-methyladenine(m^(6)A)modification-related genes,methyltransferase 14(METTL14),and YTH domain family protein 1(YTHDF1),on the proliferation,migration and apoptosis capabilities of...Objective:To explore the effects of N6-methyladenine(m^(6)A)modification-related genes,methyltransferase 14(METTL14),and YTH domain family protein 1(YTHDF1),on the proliferation,migration and apoptosis capabilities of cervical cancer cells and investigate their correlation with programmed cell death-ligand 1(PD-L1)expression.Methods:The expression levels of METTL14,YTHDF1 and PD-L1 in cervical cancer tissues and normal cervical tissues were analyzed using immunohistochemistry.Small interfering RNA(siRNA)was used to knock down the expression of METTL14 and YTHDF1 genes in cervical cancer cells,and the knockdown efficiency was validated by real-time fluorescent quantitative PCR(qPCR).After knockdown of METTL14 and YTHDF1,cell proliferation was assessed by CCK-8 assay,cell migration was examined by Transwell assay,cell apoptosis was detected by flow cytometry,and PD-L1 mRNA and protein expression were evaluated using qPCR and Western blotting,respectively.Results:Immunohistochemistry results demonstrated high expression of METTL14,YTHDF1,and PD-L1 in cervical cancer tissues.Knockdown of METTL14 and YTHDF1 significantly inhibited the proliferation and migration capabilities of cervical cancer cells,increased apoptosis,and downregulated PD-L1 mRNA and protein expression levels.Conclusion:m^(6)A methylation modification can affect the proliferation,migration and apoptosis of cervical cancer cells by regulating the expression of PD-L1 in cervical cancer cells.展开更多
为探究CO_(2)浓度升高和不同氮肥水平下源库处理对粳稻茎鞘非结构性碳水化合物(NSC)积累和转运的影响,利用开顶式气室(OTC),设置2个CO_(2)浓度([CO_(2)]):对照(背景大气,a[CO_(2)])和在背景大气[CO_(2)]基础上升高200μmol·mol^(-1...为探究CO_(2)浓度升高和不同氮肥水平下源库处理对粳稻茎鞘非结构性碳水化合物(NSC)积累和转运的影响,利用开顶式气室(OTC),设置2个CO_(2)浓度([CO_(2)]):对照(背景大气,a[CO_(2)])和在背景大气[CO_(2)]基础上升高200μmol·mol^(-1)(e[CO_(2)])。以常规粳稻"南粳9108"为试验材料,在OTC内采用盆栽方式,设置低N(N1,10 g N·m^(-2))、中N(N2,20 g N·m^(-2))和高N(N3,30 g N·m^(-2))3个施N水平。抽穗期源库改变设剪叶(LC)和疏花(SR)处理,以不处理为对照。测定并计算了抽穗期和成熟期叶片N含量、茎鞘NSC积累量(TM_(NSC))、NSC表观转运量(ATMNSC)及其对籽粒产量的表观贡献率(AC_(NSC))。采用方差分析、相关分析和逐步回归方法对上述观测数据进行分析。结果表明,[CO_(2)]升高显著降低抽穗期叶片N含量,显著促进中N水平的NSC积累。在不同[CO_(2)]和N水平下,SR处理均导致成熟期茎鞘TM_(NSC)显著升高,ATM_(NSC)和AC_(NSC)显著降低;在背景大气和不同N水平下,LC处理均显著降低成熟期TM_(NSC),显著提高ATM_(NSC),但[CO_(2)]升高下LC处理对成熟期TM_(NSC)和ATM_(NSC)均无显著影响。LC处理对籽粒产量及其构成未产生显著影响。粒叶比越高,成熟期TM_(NSC)和千粒重越低,ATM_(NSC)、AC_(NSC)、籽粒产量和收获指数越高。综合影响AC_(NSC)的因素为粒叶比、抽穗期和成熟期TM_(NSC);综合影响籽粒产量的因素为粒叶比、成熟期叶片N含量和TM_(NSC),这些综合影响均可用多元回归模型定量表述。展开更多
基金National Natural Science Foundation of China (No.81472431)Jiangsu Provincial Medical Key Talent Fund (No.ZDRCA2016072)Natural Science Foundation of Nanjing University of Traditional Chinese Medicine (No.XZR2020070)。
文摘Objective:To explore the effects of N6-methyladenine(m^(6)A)modification-related genes,methyltransferase 14(METTL14),and YTH domain family protein 1(YTHDF1),on the proliferation,migration and apoptosis capabilities of cervical cancer cells and investigate their correlation with programmed cell death-ligand 1(PD-L1)expression.Methods:The expression levels of METTL14,YTHDF1 and PD-L1 in cervical cancer tissues and normal cervical tissues were analyzed using immunohistochemistry.Small interfering RNA(siRNA)was used to knock down the expression of METTL14 and YTHDF1 genes in cervical cancer cells,and the knockdown efficiency was validated by real-time fluorescent quantitative PCR(qPCR).After knockdown of METTL14 and YTHDF1,cell proliferation was assessed by CCK-8 assay,cell migration was examined by Transwell assay,cell apoptosis was detected by flow cytometry,and PD-L1 mRNA and protein expression were evaluated using qPCR and Western blotting,respectively.Results:Immunohistochemistry results demonstrated high expression of METTL14,YTHDF1,and PD-L1 in cervical cancer tissues.Knockdown of METTL14 and YTHDF1 significantly inhibited the proliferation and migration capabilities of cervical cancer cells,increased apoptosis,and downregulated PD-L1 mRNA and protein expression levels.Conclusion:m^(6)A methylation modification can affect the proliferation,migration and apoptosis of cervical cancer cells by regulating the expression of PD-L1 in cervical cancer cells.
文摘为探究CO_(2)浓度升高和不同氮肥水平下源库处理对粳稻茎鞘非结构性碳水化合物(NSC)积累和转运的影响,利用开顶式气室(OTC),设置2个CO_(2)浓度([CO_(2)]):对照(背景大气,a[CO_(2)])和在背景大气[CO_(2)]基础上升高200μmol·mol^(-1)(e[CO_(2)])。以常规粳稻"南粳9108"为试验材料,在OTC内采用盆栽方式,设置低N(N1,10 g N·m^(-2))、中N(N2,20 g N·m^(-2))和高N(N3,30 g N·m^(-2))3个施N水平。抽穗期源库改变设剪叶(LC)和疏花(SR)处理,以不处理为对照。测定并计算了抽穗期和成熟期叶片N含量、茎鞘NSC积累量(TM_(NSC))、NSC表观转运量(ATMNSC)及其对籽粒产量的表观贡献率(AC_(NSC))。采用方差分析、相关分析和逐步回归方法对上述观测数据进行分析。结果表明,[CO_(2)]升高显著降低抽穗期叶片N含量,显著促进中N水平的NSC积累。在不同[CO_(2)]和N水平下,SR处理均导致成熟期茎鞘TM_(NSC)显著升高,ATM_(NSC)和AC_(NSC)显著降低;在背景大气和不同N水平下,LC处理均显著降低成熟期TM_(NSC),显著提高ATM_(NSC),但[CO_(2)]升高下LC处理对成熟期TM_(NSC)和ATM_(NSC)均无显著影响。LC处理对籽粒产量及其构成未产生显著影响。粒叶比越高,成熟期TM_(NSC)和千粒重越低,ATM_(NSC)、AC_(NSC)、籽粒产量和收获指数越高。综合影响AC_(NSC)的因素为粒叶比、抽穗期和成熟期TM_(NSC);综合影响籽粒产量的因素为粒叶比、成熟期叶片N含量和TM_(NSC),这些综合影响均可用多元回归模型定量表述。
