【据《Hepatology》2019年1月】报道:肝细胞E1A激活基因阻遏子通过转化生长因子激酶1依赖减轻小鼠肝缺血再灌注损伤(作者 Yang L等)肝缺血再灌注损伤是在肝移植、肝切除手术中不可避免的损伤过程,是肝脏外科手术的一大难题。目前唯一有...【据《Hepatology》2019年1月】报道:肝细胞E1A激活基因阻遏子通过转化生长因子激酶1依赖减轻小鼠肝缺血再灌注损伤(作者 Yang L等)肝缺血再灌注损伤是在肝移植、肝切除手术中不可避免的损伤过程,是肝脏外科手术的一大难题。目前唯一有效的治疗方案是缺血预处理,但在适应症上存在缺陷。因此,对肝缺血再灌注损伤发病机制的深入了解并开发有效的治疗方法至关重要。E1A激活基因阻遏子(CREG)是细胞增殖的关键调节因子,已有相关文献报道其在心血管疾病中发挥重要的保护作用,同时也可在肝脂质积累和炎症反应中发挥保护作用。然而,其在肝缺血再灌注损伤中的作用尚未见报道。因此,探讨CREG在肝缺血再灌注损伤中的作用可为临床诊疗提供新的思路。展开更多
DNA markers play important roles in plant breed- ing and genetics. The Insertion/Deletion （InDel） marker is one kind of co-dominant DNA markers widely used due to its low cost and high precision. However, the canoni...DNA markers play important roles in plant breed- ing and genetics. The Insertion/Deletion （InDel） marker is one kind of co-dominant DNA markers widely used due to its low cost and high precision. However, the canonical way of searching for InDel markers is time-consuming and labor- intensive. We developed an end-to-end computational solution （InDel Markers Development Platform, IMDP） to identify genome-wide InDel markers under a graphic pipeline environment. IMDP constitutes assembled genome sequen- ces alignment pipeline （AGA-pipe） and next-generation re- sequencing data mapping pipeline （NGS-pipe）. With AGA-pipe we are able to identify 12,944 markers between the genome of rice cultivars Nipponbare and 93-11. Using NGS-pipe, we reported 34,794 InDels from re-sequencing data of rice cultivars Wu-Yun-Geng7 and Guang-Lu-Ai4. Combining AGA- pipe and NGS-pipe, we developed 2o5,659 InDels in eight japonica and nine indica cultivars and 2,681 InDels showed a subgroup-specific pattern. Polymerase chain reaction （PCR） analysis of subgroup-specific markers indicated that the precision reached 90% （86 of 95）. Finally, to make them available to the public, we have integrated the InDels/markers information into a website （Rice InDel Marker Database, RIMD, http：I/2o2.12o.45.71/）. The application of IMDP in rice will facilitate efficiency for development of genome-wide InDel markers, in addition it can be used in other species with reference genome sequences and NGS data.展开更多
文摘【据《Hepatology》2019年1月】报道:肝细胞E1A激活基因阻遏子通过转化生长因子激酶1依赖减轻小鼠肝缺血再灌注损伤(作者 Yang L等)肝缺血再灌注损伤是在肝移植、肝切除手术中不可避免的损伤过程,是肝脏外科手术的一大难题。目前唯一有效的治疗方案是缺血预处理,但在适应症上存在缺陷。因此,对肝缺血再灌注损伤发病机制的深入了解并开发有效的治疗方法至关重要。E1A激活基因阻遏子(CREG)是细胞增殖的关键调节因子,已有相关文献报道其在心血管疾病中发挥重要的保护作用,同时也可在肝脂质积累和炎症反应中发挥保护作用。然而,其在肝缺血再灌注损伤中的作用尚未见报道。因此,探讨CREG在肝缺血再灌注损伤中的作用可为临床诊疗提供新的思路。
基金supported by the Funds from National Natural Science Foundation of China(31270222,31470397 and 31230051)Key Project on Basic Research from Science and Technology Commission of Shanghai(14JC1403900)+5 种基金Project on Breeding from Agriculture Commission of Shanghai(2013-13)the China Innovative Research Team,Ministry of Education,Chinathe 111 Project (B14016)the Innovation Program of Shanghai Municipal Education Commission(13ZZ018)the Innovation Program of Shanghai Pudong Science and Technology Commission (PKJ2013-N03)National Transgenic Major Program Grants 2014ZX08009-003-003
文摘DNA markers play important roles in plant breed- ing and genetics. The Insertion/Deletion （InDel） marker is one kind of co-dominant DNA markers widely used due to its low cost and high precision. However, the canonical way of searching for InDel markers is time-consuming and labor- intensive. We developed an end-to-end computational solution （InDel Markers Development Platform, IMDP） to identify genome-wide InDel markers under a graphic pipeline environment. IMDP constitutes assembled genome sequen- ces alignment pipeline （AGA-pipe） and next-generation re- sequencing data mapping pipeline （NGS-pipe）. With AGA-pipe we are able to identify 12,944 markers between the genome of rice cultivars Nipponbare and 93-11. Using NGS-pipe, we reported 34,794 InDels from re-sequencing data of rice cultivars Wu-Yun-Geng7 and Guang-Lu-Ai4. Combining AGA- pipe and NGS-pipe, we developed 2o5,659 InDels in eight japonica and nine indica cultivars and 2,681 InDels showed a subgroup-specific pattern. Polymerase chain reaction （PCR） analysis of subgroup-specific markers indicated that the precision reached 90% （86 of 95）. Finally, to make them available to the public, we have integrated the InDels/markers information into a website （Rice InDel Marker Database, RIMD, http：I/2o2.12o.45.71/）. The application of IMDP in rice will facilitate efficiency for development of genome-wide InDel markers, in addition it can be used in other species with reference genome sequences and NGS data.
