目的运用美国FDA不良事件报告系统(FAERS)挖掘艾塞那肽微球使用中不良事件(ADE)风险信号,为临床合理用药和保障用药安全提供参考。方法以“艾塞那肽微球”为目标药物,检索关键词为“exenatide microspheres for injection”“LY05006”...目的运用美国FDA不良事件报告系统(FAERS)挖掘艾塞那肽微球使用中不良事件(ADE)风险信号,为临床合理用药和保障用药安全提供参考。方法以“艾塞那肽微球”为目标药物,检索关键词为“exenatide microspheres for injection”“LY05006”“AC 2993 LAR”及“bydureon”,利用SAS软件提取FAERS数据库中2012年1月2日至2023年3月31日的ADE报告数据并去重,采用报告比值比法与综合标准法对艾塞那肽微球相关ADE报告进行数据挖掘。结果共检索到艾塞那肽微球ADE报告27248份,涉及27个系统器官分类,其中严重ADE报告4719份。上报人员以消费者为主(18435例,占67.66%),上报国家以美国为主(26295例,占96.50%)。采用报告比值比法(ROR)与综合标准法(MHRA)共获得163个ADE风险信号,包括血胆固醇异常、脂肪酶升高及混合型高脂血症等新发不良反应。结论基于FAERS数据库对艾塞那肽微球上市后的ADE进行挖掘分析,可对临床用药安全和提高患者用药依从性提供参考。展开更多
Objective To study the alteration of circulating microRNAs in 4-（methylnitrosamino）-l-（3-pyridyl） -l-butanone （NNK）-induced early stage lung carcinogenesis. Methods A lung cancer model of male F344 rats was indu...Objective To study the alteration of circulating microRNAs in 4-（methylnitrosamino）-l-（3-pyridyl） -l-butanone （NNK）-induced early stage lung carcinogenesis. Methods A lung cancer model of male F344 rats was induced with systemic NNK and levels of 8 lung cancer-associated miRNAs in whole blood and serum of rats were measured by quantitative RT-PCR of each at weeks 2, 5, 20, and 20 following NNK treatment. Results No lung cancer was detected in control group and NNK treatment group at week 20 following NNK treatment. The levels of some circulating miRNAs were significantly higher in NNK treatment group than in control group. The miR-210 was down-regulated and the miR-206 was up-regulated in NNK treatment group. The expression level of circulating miRNAs changed from week 1 to week 20 following NNK treatment. Conclusion The expression level of circulating miRNAs is related to NNK-induced early stage lung carcinogenesis in rats and can therefore serve as its potential indicator.展开更多
目的:通过网络药理学方法预测和探讨益气活血方抗肝纤维化的活性成分及其潜在的作用机制。方法:采用中药系统药理学数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)、中医...目的:通过网络药理学方法预测和探讨益气活血方抗肝纤维化的活性成分及其潜在的作用机制。方法:采用中药系统药理学数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)、中医药综合数据库(Traditional Chinese Medicine Integrated Database,TCMID)筛选出益气活血方的活性成分和相关的潜在靶点;利用GeneCards数据库检索肝纤维化相关靶点;基于益气活血方潜在靶点与肝纤维化相关靶点的匹配结果,利用String平台进行蛋白质相互作用分析,构建蛋白质相互作用网络,拓扑分析筛选核心靶点;利用Cytoscape 3.7.2构建益气活血方活性成分-肝纤维化-靶点网络,拓扑分析筛选出关键活性成分;再利用R语言软件对药物与疾病共有靶点分别进行基因本体(gene ontology,GO)功能、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。结果:益气活血方抗肝纤维化的核心活性成分为槲皮素、木犀草素、山奈酚、柚皮素、丹参酮Ⅱa等。核心靶点有转录激活因子-3(signal transducer and activator of transcription-3,STAT3)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、丝氨酸/苏氨酸蛋白激酶-1(serine/threonine protein kinase-1,AKT1)、原癌基因(proto-oncogene,JUN)、抑癌基因TP53等,主要涉及营养元素和脂多糖的应答过程、氧化应激反应、类固醇代谢应答过程、脂多糖的反应、活性氧代谢过程、小分子代谢过程的调控等生物过程。主要富集在磷脂酰肌醇-4,5-二磷酸3-激酶-蛋白激酶B(phosphatidylinositol-4,5-biphosphate 3-kinase-protein kinase B,PI3K-Akt)、流体剪应力与动脉粥样硬化、糖尿病并发症中的糖基化终末产物及其受体(advanced glycation and products-receptor of AGE,AGE-RAGE)、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)、TNF-α、白介素17(interleukin-17,IL-17)等信号通路。结论:益气活血方抗肝纤维化具有多成分、多靶点、多通路的作用特点,本研究为益气活血方抗肝纤维化机制的后续实验研究提供了理论基础和参考依据。展开更多
目的探讨不同手术方式治疗输尿管结石的效果。方法方便选取2017年3月—2018年6月贵阳医学院第二附属医院收治的90例输尿管结石患者,随机分为研究组和对照组。对照组采用腹腔镜下输尿管切开取石术治疗。研究组采用输尿管镜下钬激光碎石...目的探讨不同手术方式治疗输尿管结石的效果。方法方便选取2017年3月—2018年6月贵阳医学院第二附属医院收治的90例输尿管结石患者,随机分为研究组和对照组。对照组采用腹腔镜下输尿管切开取石术治疗。研究组采用输尿管镜下钬激光碎石术治疗。记录两组的手术时间、手术失血、总住院天数。记录两组的首次碎石成功、二次手术、结石清除情况。对比两组的并发症发生率。结果研究组的手术时间(38.15±5.49)min vs (52.08±6.72)min、手术失血(13.25±3.14)mL vs (41.09±6.53)mL、总住院天数(3.74±0.85)d vs (6.95±1.34)d低于对照组,差异有统计学意义(t=10.769、25.775、13.570,P<0.05);研究组的首次碎石成功(77.78%vs 93.33%)、结石清除(20.00%vs 2.22%)低于对照组,但二次手术率(82.22%vs 97.78%)比对照组高,差异有统计学意义(P<0.05);研究组的并发症发生率低于对照组(11.11%vs 26.67%),差异有统计学意义(P<0.05)。结论腹腔镜下输尿管切开取石术具有结石清除率高、并发症高的特点,输尿管镜下钬激光碎石术具有手术时间短、出血量低、恢复快、并发症少等特点,但结石清除率较低,需进行二次手术率较高。展开更多
基金supported by National Key Basic Research Program of China(2012CB525004 to J.Y.)National Natural Science Foundation of China(30972443 to J.Y.)+6 种基金Research Fund for Doctoral Program of Higher Education of China(20114423110002 to J.Y.)Key Program of Guangdong Natural Science Foundation(9251018201000004 to J.Y.)University Talent Program of Guangdong(2010-79 to J.Y.)University Talent Program of Guangzhou(10A003D to J.Y.)Science and Technology Project of Guangzhou(2010Y1-C441 to J.Y.)Youth Foundation of China State Key Laboratory of Respiratory Disease(201105 to W.J.)Medical Scientific Research Foundation of Guangdong Province(A2011234 to W.J.)
文摘Objective To study the alteration of circulating microRNAs in 4-（methylnitrosamino）-l-（3-pyridyl） -l-butanone （NNK）-induced early stage lung carcinogenesis. Methods A lung cancer model of male F344 rats was induced with systemic NNK and levels of 8 lung cancer-associated miRNAs in whole blood and serum of rats were measured by quantitative RT-PCR of each at weeks 2, 5, 20, and 20 following NNK treatment. Results No lung cancer was detected in control group and NNK treatment group at week 20 following NNK treatment. The levels of some circulating miRNAs were significantly higher in NNK treatment group than in control group. The miR-210 was down-regulated and the miR-206 was up-regulated in NNK treatment group. The expression level of circulating miRNAs changed from week 1 to week 20 following NNK treatment. Conclusion The expression level of circulating miRNAs is related to NNK-induced early stage lung carcinogenesis in rats and can therefore serve as its potential indicator.
文摘目的:通过网络药理学方法预测和探讨益气活血方抗肝纤维化的活性成分及其潜在的作用机制。方法:采用中药系统药理学数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)、中医药综合数据库(Traditional Chinese Medicine Integrated Database,TCMID)筛选出益气活血方的活性成分和相关的潜在靶点;利用GeneCards数据库检索肝纤维化相关靶点;基于益气活血方潜在靶点与肝纤维化相关靶点的匹配结果,利用String平台进行蛋白质相互作用分析,构建蛋白质相互作用网络,拓扑分析筛选核心靶点;利用Cytoscape 3.7.2构建益气活血方活性成分-肝纤维化-靶点网络,拓扑分析筛选出关键活性成分;再利用R语言软件对药物与疾病共有靶点分别进行基因本体(gene ontology,GO)功能、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。结果:益气活血方抗肝纤维化的核心活性成分为槲皮素、木犀草素、山奈酚、柚皮素、丹参酮Ⅱa等。核心靶点有转录激活因子-3(signal transducer and activator of transcription-3,STAT3)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、丝氨酸/苏氨酸蛋白激酶-1(serine/threonine protein kinase-1,AKT1)、原癌基因(proto-oncogene,JUN)、抑癌基因TP53等,主要涉及营养元素和脂多糖的应答过程、氧化应激反应、类固醇代谢应答过程、脂多糖的反应、活性氧代谢过程、小分子代谢过程的调控等生物过程。主要富集在磷脂酰肌醇-4,5-二磷酸3-激酶-蛋白激酶B(phosphatidylinositol-4,5-biphosphate 3-kinase-protein kinase B,PI3K-Akt)、流体剪应力与动脉粥样硬化、糖尿病并发症中的糖基化终末产物及其受体(advanced glycation and products-receptor of AGE,AGE-RAGE)、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)、TNF-α、白介素17(interleukin-17,IL-17)等信号通路。结论:益气活血方抗肝纤维化具有多成分、多靶点、多通路的作用特点,本研究为益气活血方抗肝纤维化机制的后续实验研究提供了理论基础和参考依据。
文摘目的探讨不同手术方式治疗输尿管结石的效果。方法方便选取2017年3月—2018年6月贵阳医学院第二附属医院收治的90例输尿管结石患者,随机分为研究组和对照组。对照组采用腹腔镜下输尿管切开取石术治疗。研究组采用输尿管镜下钬激光碎石术治疗。记录两组的手术时间、手术失血、总住院天数。记录两组的首次碎石成功、二次手术、结石清除情况。对比两组的并发症发生率。结果研究组的手术时间(38.15±5.49)min vs (52.08±6.72)min、手术失血(13.25±3.14)mL vs (41.09±6.53)mL、总住院天数(3.74±0.85)d vs (6.95±1.34)d低于对照组,差异有统计学意义(t=10.769、25.775、13.570,P<0.05);研究组的首次碎石成功(77.78%vs 93.33%)、结石清除(20.00%vs 2.22%)低于对照组,但二次手术率(82.22%vs 97.78%)比对照组高,差异有统计学意义(P<0.05);研究组的并发症发生率低于对照组(11.11%vs 26.67%),差异有统计学意义(P<0.05)。结论腹腔镜下输尿管切开取石术具有结石清除率高、并发症高的特点,输尿管镜下钬激光碎石术具有手术时间短、出血量低、恢复快、并发症少等特点,但结石清除率较低,需进行二次手术率较高。
