With the assumption of the Park model that the wake region is in linear expansion and the cross-wind is in multinomial and Gaussian distribution in wake region,one develops the Park-polynomial model and the ParkGaussi...With the assumption of the Park model that the wake region is in linear expansion and the cross-wind is in multinomial and Gaussian distribution in wake region,one develops the Park-polynomial model and the ParkGaussian model to numerically simulate the wake flow field for a single wind turbine.Compared with the measured data of wind farm and the wind tunnel test,it shows that the prediction precision of wake field has been improved obviously under the modified initial wake radius.Moreover,both of the newly modified two models could well simulate the wind velocity in wake region,because the predicted results is approximately consistent with the test result,and the cross-wind distribution conforms to that of the real flow field.The two models have still inherited many advantages of engineering models,such as simple form,easy-to-code,and high computational efficiency.Particularly,the Park-Gaussian model is the best in overall performance among them.展开更多
A psychrophilic bacterium strain 547 producing cold-adaptive alkaline protease was isolated from the deep sea sediment of Prydz Bay, Antarctica. The organism was identified as a Planomicrobium species by 16S rRNA anal...A psychrophilic bacterium strain 547 producing cold-adaptive alkaline protease was isolated from the deep sea sediment of Prydz Bay, Antarctica. The organism was identified as a Planomicrobium species by 16S rRNA analysis. The optimal and highest growth temperatures for strain 547 were 15~C and 30~C, respectively. The extracellular protease was purified by ammonium sulfate precipitation and DEAE cellulose-52 chromatography. The optimal temperature and pH for the activity of the purified enzyme were 35~C and pH 9.0, respectively. The enzyme retained approximately 40% of its activity after 2 h of incubation at 50℃. The enzymatic activity was inhibited by 1 mmol/L phenylmethyl sulfonylfluoride （PMSF） and hydrochloride 4-（2-aminoethyl）-benzenesulfonyl fluoride （AEBSF）, indicating that it was a serine protease. The presence of Cae＋ and Mnz＋ increased the activity of the enzyme. The protease gene with a size of 1 269 bp was cloned from Planomicrobium sp. 547 using primers designed based on the conserved sequences of proteases in GenBank. The Planomicrobium sp. 547 protease contained a domain belonging to the peptidase S8 family, which has a length of 309 amino acid （AA） residues. The alignment and phylogenetic analysis of the AA sequence indicated that the protease belonged to the subtilisin family.展开更多
A protocol to coextract the microbial metagenomic DNA and RNA from deep-sea sediment was developed for the microbiological study of environmental samples. The obtained pure metagenomic DNA with the size larger than 23...A protocol to coextract the microbial metagenomic DNA and RNA from deep-sea sediment was developed for the microbiological study of environmental samples. The obtained pure metagenomic DNA with the size larger than 23 kb and stable RNA could be used directly for PCR and reverse transcription - PCR （ RT - PCR） respectively. The direct lysis including the treatments of SDS, proteinase and lysozyme was applied to acquiring the metagenomic DNA and RNA furthest. Prior to the lysis treatment, the glass bead and denaturing solution were added to enhance the lysis efficiency and keep the integrity of RNA respectively. Denaturing gradient gel electrophoresis （DGGE） was applied in accessing the microbial 16S rRNA diversity by PCR and RT -PCR amplification from a single extraction. The pattern obtained by this analysis revealed some differences between them, indicating the efficiency of the protocol in extracting the metagenomic DNA and total RNA from deep-sea sediment.展开更多
基金funded jointly by the National Basic Research Program of China(″973″Program)(No2014CB046200)the Natural Science Foundation of Jiangsu Province(BK2014059)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘With the assumption of the Park model that the wake region is in linear expansion and the cross-wind is in multinomial and Gaussian distribution in wake region,one develops the Park-polynomial model and the ParkGaussian model to numerically simulate the wake flow field for a single wind turbine.Compared with the measured data of wind farm and the wind tunnel test,it shows that the prediction precision of wake field has been improved obviously under the modified initial wake radius.Moreover,both of the newly modified two models could well simulate the wind velocity in wake region,because the predicted results is approximately consistent with the test result,and the cross-wind distribution conforms to that of the real flow field.The two models have still inherited many advantages of engineering models,such as simple form,easy-to-code,and high computational efficiency.Particularly,the Park-Gaussian model is the best in overall performance among them.
基金supported by the National High Technology Research and Development Program of China (Grant no. 2007AA091407)the Project of China Ocean Mineral Resources Research and Development Association (COMRA, Grant no. DYXM-115-02-2-04)
文摘A psychrophilic bacterium strain 547 producing cold-adaptive alkaline protease was isolated from the deep sea sediment of Prydz Bay, Antarctica. The organism was identified as a Planomicrobium species by 16S rRNA analysis. The optimal and highest growth temperatures for strain 547 were 15~C and 30~C, respectively. The extracellular protease was purified by ammonium sulfate precipitation and DEAE cellulose-52 chromatography. The optimal temperature and pH for the activity of the purified enzyme were 35~C and pH 9.0, respectively. The enzyme retained approximately 40% of its activity after 2 h of incubation at 50℃. The enzymatic activity was inhibited by 1 mmol/L phenylmethyl sulfonylfluoride （PMSF） and hydrochloride 4-（2-aminoethyl）-benzenesulfonyl fluoride （AEBSF）, indicating that it was a serine protease. The presence of Cae＋ and Mnz＋ increased the activity of the enzyme. The protease gene with a size of 1 269 bp was cloned from Planomicrobium sp. 547 using primers designed based on the conserved sequences of proteases in GenBank. The Planomicrobium sp. 547 protease contained a domain belonging to the peptidase S8 family, which has a length of 309 amino acid （AA） residues. The alignment and phylogenetic analysis of the AA sequence indicated that the protease belonged to the subtilisin family.
文摘A protocol to coextract the microbial metagenomic DNA and RNA from deep-sea sediment was developed for the microbiological study of environmental samples. The obtained pure metagenomic DNA with the size larger than 23 kb and stable RNA could be used directly for PCR and reverse transcription - PCR （ RT - PCR） respectively. The direct lysis including the treatments of SDS, proteinase and lysozyme was applied to acquiring the metagenomic DNA and RNA furthest. Prior to the lysis treatment, the glass bead and denaturing solution were added to enhance the lysis efficiency and keep the integrity of RNA respectively. Denaturing gradient gel electrophoresis （DGGE） was applied in accessing the microbial 16S rRNA diversity by PCR and RT -PCR amplification from a single extraction. The pattern obtained by this analysis revealed some differences between them, indicating the efficiency of the protocol in extracting the metagenomic DNA and total RNA from deep-sea sediment.
