目的探讨超声引导下靶点注射甲钴胺对周围神经损伤后神经电生理、超声下神经结构、感觉功能的影响。方法本试验将周围神经损伤后功能障碍患者按照1︰1随机纳入试验组及对照组,其中试验组13例,男性9例,女性4例;平均年龄为(33.92±7....目的探讨超声引导下靶点注射甲钴胺对周围神经损伤后神经电生理、超声下神经结构、感觉功能的影响。方法本试验将周围神经损伤后功能障碍患者按照1︰1随机纳入试验组及对照组,其中试验组13例,男性9例,女性4例;平均年龄为(33.92±7.54)岁。对照组12例,男9例,女3例;平均年龄为(33.08±11.31)岁。试验组采用超声引导下靶点注射甲钴胺注射液结合常规治疗,对照组采用常规治疗,于治疗前和治疗12周分别进行神经电生理(神经潜伏期、波幅、传导速度)、超声下神经直径及横截面积、英国医学研究委员会(British medical research council,BMRC)感觉功能评定。结果与治疗前对比,两组患者的神经电生理(神经潜伏期、波幅、传导速度)、超声下神经直径及横截面积、神经BMRC感觉评定在治疗后差异有统计学意义(P<0.05);与对照组对比,试验组的超声下神经直径及横截面积、神经BMRC感觉在治疗前后的改变差异有统计学意义(P<0.05)。结论超声引导下靶点注射甲钴胺结合常规治疗对周围神经结构、感觉功能的改善优于常规治疗。展开更多
Objective:To investigate the effects of electro-acupuncture preconditioning on focal cerebral blood flow(CBF)in artery and vein of rats with middle cerebral artery occlusion(MCAO),and to provide experimental evidences...Objective:To investigate the effects of electro-acupuncture preconditioning on focal cerebral blood flow(CBF)in artery and vein of rats with middle cerebral artery occlusion(MCAO),and to provide experimental evidences for primary prevention of ischemic stroke.Method:Eighteen male Sprague-Dawley rats(two months old)were divided into 3 groups:electro-acupuncture preconditioning group(EA group),ischemia group(Is group)and Dazhui and Baihui sham group(six rats per group).The rats in the EA group were given electro-acupuncture preconditioning at Dazhui and Baihui with 2/15 density wave current for 30 minutes per day,5 days consecutively.After preconditioning,enhanced laser speckle contrast analysis was implemented to get false color images before making middle cerebral ischemia occlusion model.Then getting false color images two hours during ischemia by laser speckle contrast analysis again.Result:④Relative CBF in focal cortical artery:There were significant differences at every time point in EA group and Is group(P<0.01,P<0.05)comparing with that before occlusion respectively.Compared with that before ischemia,there was significant difference at 120 min after ischemia in sham group(P<0.05);compared with that at 30 min after occlusion,there was significant difference at every time point in EA group from the time point 60 min after occlusion;there was significant difference at every time point in EA group and Is group(P<0.05)comparing with sham group;Compared with sham group,there was significant difference at 30min and 60 min after ischemia in EA group(P<0.05).④Relative CBF in focal cortical vein:There was significant difference at every time point in sham group comparing with that before occlusion(P<0.05);compared with that before occlusion,there was significant difference from 45 min after occlusion in EA group(P<0.05);compared with that before occlusion,there was significant difference from 105 min after occlusion in sham group(P<0.05);there was significant difference at 120 min after occlusion comparing with 60 min after occlusion in EA group;compared with sham group,there was significant difference at every time point in EA group and Is group(except 30 min after occlusion)(P<0.05),respectively;compared with Is group,there was significant difference at 30,45,60 min after occlusion in EA group(P<0.05).④The infarct brain volume detected 24 h after reperfusion in EA group was significantly smaller comparing with that in Is group(P<0.05).Conclusion:Electro-acupuncture preconditioning could increase CBF velocity in artery and vein of focal cortex in rats with MCAO,which also had time-dependence.Additionally,electro-acupuncture preconditioning could decrease the infarct volume detected 24h after reperfusion,which possibly was correlated with the blood supply in ischemic penumbra.展开更多
文摘目的探讨超声引导下靶点注射甲钴胺对周围神经损伤后神经电生理、超声下神经结构、感觉功能的影响。方法本试验将周围神经损伤后功能障碍患者按照1︰1随机纳入试验组及对照组,其中试验组13例,男性9例,女性4例;平均年龄为(33.92±7.54)岁。对照组12例,男9例,女3例;平均年龄为(33.08±11.31)岁。试验组采用超声引导下靶点注射甲钴胺注射液结合常规治疗,对照组采用常规治疗,于治疗前和治疗12周分别进行神经电生理(神经潜伏期、波幅、传导速度)、超声下神经直径及横截面积、英国医学研究委员会(British medical research council,BMRC)感觉功能评定。结果与治疗前对比,两组患者的神经电生理(神经潜伏期、波幅、传导速度)、超声下神经直径及横截面积、神经BMRC感觉评定在治疗后差异有统计学意义(P<0.05);与对照组对比,试验组的超声下神经直径及横截面积、神经BMRC感觉在治疗前后的改变差异有统计学意义(P<0.05)。结论超声引导下靶点注射甲钴胺结合常规治疗对周围神经结构、感觉功能的改善优于常规治疗。
基金supported by the Natural Science Foundation of China(NSFC,NO.81171856,81171855)the Development Program of China(863 program,NO.2007AA02Z482)the Key projects of Shanghai Science and Technology on Biomedicine(NO.10DZ1950800)
文摘Objective:To investigate the effects of electro-acupuncture preconditioning on focal cerebral blood flow(CBF)in artery and vein of rats with middle cerebral artery occlusion(MCAO),and to provide experimental evidences for primary prevention of ischemic stroke.Method:Eighteen male Sprague-Dawley rats(two months old)were divided into 3 groups:electro-acupuncture preconditioning group(EA group),ischemia group(Is group)and Dazhui and Baihui sham group(six rats per group).The rats in the EA group were given electro-acupuncture preconditioning at Dazhui and Baihui with 2/15 density wave current for 30 minutes per day,5 days consecutively.After preconditioning,enhanced laser speckle contrast analysis was implemented to get false color images before making middle cerebral ischemia occlusion model.Then getting false color images two hours during ischemia by laser speckle contrast analysis again.Result:④Relative CBF in focal cortical artery:There were significant differences at every time point in EA group and Is group(P<0.01,P<0.05)comparing with that before occlusion respectively.Compared with that before ischemia,there was significant difference at 120 min after ischemia in sham group(P<0.05);compared with that at 30 min after occlusion,there was significant difference at every time point in EA group from the time point 60 min after occlusion;there was significant difference at every time point in EA group and Is group(P<0.05)comparing with sham group;Compared with sham group,there was significant difference at 30min and 60 min after ischemia in EA group(P<0.05).④Relative CBF in focal cortical vein:There was significant difference at every time point in sham group comparing with that before occlusion(P<0.05);compared with that before occlusion,there was significant difference from 45 min after occlusion in EA group(P<0.05);compared with that before occlusion,there was significant difference from 105 min after occlusion in sham group(P<0.05);there was significant difference at 120 min after occlusion comparing with 60 min after occlusion in EA group;compared with sham group,there was significant difference at every time point in EA group and Is group(except 30 min after occlusion)(P<0.05),respectively;compared with Is group,there was significant difference at 30,45,60 min after occlusion in EA group(P<0.05).④The infarct brain volume detected 24 h after reperfusion in EA group was significantly smaller comparing with that in Is group(P<0.05).Conclusion:Electro-acupuncture preconditioning could increase CBF velocity in artery and vein of focal cortex in rats with MCAO,which also had time-dependence.Additionally,electro-acupuncture preconditioning could decrease the infarct volume detected 24h after reperfusion,which possibly was correlated with the blood supply in ischemic penumbra.
