The dmrt1 (doublesex and mab-3 related transcription factor 1) gene is considered to play an essential role in testis differentiation and development among metazoan species. As an economically important marine fish in...The dmrt1 (doublesex and mab-3 related transcription factor 1) gene is considered to play an essential role in testis differentiation and development among metazoan species. As an economically important marine fish in China, Chinese tongue sole (Cynoglossus semilaevis) possess a ZW/ZZ sex-determining system, and the females usually grow two to four times bigger than males. Previous studies have confirmed that Z-linked dmrt1 gene is the male determining gene in C. semilaevis and TALENs-mediated knocking-out individuals showed increased growth phenotype. In order to investigate the function of dmrt1 regulatory regions and its potential application, we have cloned the promoter of C. semilaevis dmrt1. The acquired sequence consisted of a 5'-upstream sequence and a partial exon1;functional analysis showed that there were two positive regulatory regions in the promoter, as well as one negative region and one neutral region. These positive regions were then combined to obtain several newly constructed promoters. Both in vitro and in vivo analysis confirmed one of them (dmrt1-Δ3+Δ4) showed elevated promoting activity compared to the full-length promoter and could drive exogenous gene expression in tongue sole gonads. Our results will provide useful information for understanding the regulatory mechanism of dmrt1 gene during C. semilaevis sex determination, and the endogenous promoter will facilitate the transgenic research for analyzing sex- and growth-related genes.展开更多
This article explores the benefits of pair-drill interpretationexercises to combat the problem of interpretation on ChineseNational College campus where there is no English speakingenvironment, and a lack of realistic...This article explores the benefits of pair-drill interpretationexercises to combat the problem of interpretation on ChineseNational College campus where there is no English speakingenvironment, and a lack of realistic interpretation practice. Tocreate a lively atmosphere whilst practicing teaching English-Chinese oral interpretation, the teacher designed a system ofpair-drill interpretation scenarios and learning projects. Underthe teacher’s guidance students pair up to practice interpretationboth inside and outside the classroom.展开更多
基金Supported by the Special Scientific Research Funds for Central Non-profit Institutes,Yellow Sea Fisheries Research Institute(No.20603022016004)the Central Public-interest Scientific Institution Basal Research Fund,YSFRI,CAFS(No.20603022017003)+2 种基金the National Natural Science Foundation of China(No.31530078)the Taishan Scholar Climbing Project Fund of Shandong of China,National Natural Science Foundation Projects(No.31802275)the Post-doctoral Applied Research Project Fund of Qingdao City(No.ZQ51201617023)
文摘The dmrt1 (doublesex and mab-3 related transcription factor 1) gene is considered to play an essential role in testis differentiation and development among metazoan species. As an economically important marine fish in China, Chinese tongue sole (Cynoglossus semilaevis) possess a ZW/ZZ sex-determining system, and the females usually grow two to four times bigger than males. Previous studies have confirmed that Z-linked dmrt1 gene is the male determining gene in C. semilaevis and TALENs-mediated knocking-out individuals showed increased growth phenotype. In order to investigate the function of dmrt1 regulatory regions and its potential application, we have cloned the promoter of C. semilaevis dmrt1. The acquired sequence consisted of a 5'-upstream sequence and a partial exon1;functional analysis showed that there were two positive regulatory regions in the promoter, as well as one negative region and one neutral region. These positive regions were then combined to obtain several newly constructed promoters. Both in vitro and in vivo analysis confirmed one of them (dmrt1-Δ3+Δ4) showed elevated promoting activity compared to the full-length promoter and could drive exogenous gene expression in tongue sole gonads. Our results will provide useful information for understanding the regulatory mechanism of dmrt1 gene during C. semilaevis sex determination, and the endogenous promoter will facilitate the transgenic research for analyzing sex- and growth-related genes.
文摘This article explores the benefits of pair-drill interpretationexercises to combat the problem of interpretation on ChineseNational College campus where there is no English speakingenvironment, and a lack of realistic interpretation practice. Tocreate a lively atmosphere whilst practicing teaching English-Chinese oral interpretation, the teacher designed a system ofpair-drill interpretation scenarios and learning projects. Underthe teacher’s guidance students pair up to practice interpretationboth inside and outside the classroom.
