目的研究ABHD2在不育症患者精子膜表面的表达差异,探究孕酮是否通过ABHD2调控cAMP-PKA进而影响精子的功能活性。方法收集男性正常生育组精子、少弱精子组和顶体异常组精子样本(各30例),通过RT-PCR、蛋白Western-Blot印迹等方法分析转录...目的研究ABHD2在不育症患者精子膜表面的表达差异,探究孕酮是否通过ABHD2调控cAMP-PKA进而影响精子的功能活性。方法收集男性正常生育组精子、少弱精子组和顶体异常组精子样本(各30例),通过RT-PCR、蛋白Western-Blot印迹等方法分析转录水平及蛋白表达的差异,且采用体外添加孕酮、ABHD2抗体、PKA拟制剂H-89,选择观测正常生育组(对照组)与少弱精子组(实验组)男性精子ABHD2的蛋白表达水平,以及cAMP、PKA浓度等变化。结果少弱精子组和顶体异常组中ABHD2 m RNA转录水平以及ABHD2蛋白表达水平显著减少,具有统计学意义(F:90.84,P<0.01);在正常生育组中加入孕酮处理后,ABHD2蛋白水平表达明显增加;而少弱精子组中加入孕酮处理后,与未加孕酮相比,ABHD2蛋白水平表达增加不明显。进一步研究,在正常生育组中加入孕酮(P4)处理后,cAMP、PKA浓度极显著增加(P<0.01),继续加入ABHD2抗体或PKA拟制剂H89后,cAMP、PKA浓度均显著减少(P<0.01)。结论临床不育与正常生育男性精子之间ABHD2的转录和表达存在差异,孕酮通过ABHD2受体介导的cAMP-PKA信号通路调控精子受精功能。展开更多
Bright Cu-doped ternary ZnCdS quantum dots （ZnCdS：Cu QDs） with varied Cd concentrations were synthesized in aqueous solution by using a convenient microwave method.These ternary QDs could be directly dispersed in w...Bright Cu-doped ternary ZnCdS quantum dots （ZnCdS：Cu QDs） with varied Cd concentrations were synthesized in aqueous solution by using a convenient microwave method.These ternary QDs could be directly dispersed in water solution.By regulating the Cd2＋ concentration from 0 to 50% （mole fraction of the cations）,the photoluminescence excitation （PLE） peak of such ZnCdS：Cu QDs could be continuously redshifted from 320 nm to 380 nm,while their photoluminescence （PL） peak was redshifted from 490 nm to 580 nm,exhibiting multicolor Cu-related emissions.Furthermore,the quantum yield of the ZnCdS：Cu QDs could reach as high as 12% by regulating the Cu doping concentration.These ZnCdS：Cu ternary QDs with kind surface conditions and good composition-controllable optical properties may have potential application in many areas such as sensing,bioimaging,and light-emitting diodes.展开更多
文摘目的研究ABHD2在不育症患者精子膜表面的表达差异,探究孕酮是否通过ABHD2调控cAMP-PKA进而影响精子的功能活性。方法收集男性正常生育组精子、少弱精子组和顶体异常组精子样本(各30例),通过RT-PCR、蛋白Western-Blot印迹等方法分析转录水平及蛋白表达的差异,且采用体外添加孕酮、ABHD2抗体、PKA拟制剂H-89,选择观测正常生育组(对照组)与少弱精子组(实验组)男性精子ABHD2的蛋白表达水平,以及cAMP、PKA浓度等变化。结果少弱精子组和顶体异常组中ABHD2 m RNA转录水平以及ABHD2蛋白表达水平显著减少,具有统计学意义(F:90.84,P<0.01);在正常生育组中加入孕酮处理后,ABHD2蛋白水平表达明显增加;而少弱精子组中加入孕酮处理后,与未加孕酮相比,ABHD2蛋白水平表达增加不明显。进一步研究,在正常生育组中加入孕酮(P4)处理后,cAMP、PKA浓度极显著增加(P<0.01),继续加入ABHD2抗体或PKA拟制剂H89后,cAMP、PKA浓度均显著减少(P<0.01)。结论临床不育与正常生育男性精子之间ABHD2的转录和表达存在差异,孕酮通过ABHD2受体介导的cAMP-PKA信号通路调控精子受精功能。
基金Supported by the National Natural Science Foundation of China (10904119)
文摘Bright Cu-doped ternary ZnCdS quantum dots （ZnCdS：Cu QDs） with varied Cd concentrations were synthesized in aqueous solution by using a convenient microwave method.These ternary QDs could be directly dispersed in water solution.By regulating the Cd2＋ concentration from 0 to 50% （mole fraction of the cations）,the photoluminescence excitation （PLE） peak of such ZnCdS：Cu QDs could be continuously redshifted from 320 nm to 380 nm,while their photoluminescence （PL） peak was redshifted from 490 nm to 580 nm,exhibiting multicolor Cu-related emissions.Furthermore,the quantum yield of the ZnCdS：Cu QDs could reach as high as 12% by regulating the Cu doping concentration.These ZnCdS：Cu ternary QDs with kind surface conditions and good composition-controllable optical properties may have potential application in many areas such as sensing,bioimaging,and light-emitting diodes.
