Objective:To investigate the plasma metabolic components of patients with unstable angina(UA)and acute myocardial infarction(AMI),and to screen potential biomarkers and explore possible biological mechanisms in order ...Objective:To investigate the plasma metabolic components of patients with unstable angina(UA)and acute myocardial infarction(AMI),and to screen potential biomarkers and explore possible biological mechanisms in order to provide reference for early evaluation of acute coronary syndrome.Methods:Plasma samples from patients with UA and AMI were collected to obtain their general information,and the metabolites were detected by LCMS technology.Combined with univariate statistical analysis,the significantly different metabolites and their pathways were further determined by partial least squares discriminant analysis and orthogonalized partial least squares discriminant analysis models.Results:A total of 33 samples from UA group and 47 samples from AMI group were included for testing.Then 54 metabolites and 20 pathways were found to be significantly different between them.Metabolites such as pantoprazole,acetylcarnitine,palmitoyl ethanolamide,betaine,caprylic acid,bilirubin,histidine,oleic acid,citrate,vanillin can be used as potential biomarkers to distinguish them.The pathways include ABC transporters,aminoacyl-tRNA biosynthesis,central carbon metabolism in cancer and so on.Conclusion:There are significant differences in the plasma metabolic components of UA and AMI.Studies related to metabolomics may guide significance for exploring the biological nature of ACS.展开更多
基金National Natural Science Foundation of China(No.81973824)Leading Talents of TCM in Jiangsu Province(No.SLJ0204)The Fifth Phase of"Project 333"Scientific Research Project in Jiangsu Province(No.BRA2020386)。
文摘Objective:To investigate the plasma metabolic components of patients with unstable angina(UA)and acute myocardial infarction(AMI),and to screen potential biomarkers and explore possible biological mechanisms in order to provide reference for early evaluation of acute coronary syndrome.Methods:Plasma samples from patients with UA and AMI were collected to obtain their general information,and the metabolites were detected by LCMS technology.Combined with univariate statistical analysis,the significantly different metabolites and their pathways were further determined by partial least squares discriminant analysis and orthogonalized partial least squares discriminant analysis models.Results:A total of 33 samples from UA group and 47 samples from AMI group were included for testing.Then 54 metabolites and 20 pathways were found to be significantly different between them.Metabolites such as pantoprazole,acetylcarnitine,palmitoyl ethanolamide,betaine,caprylic acid,bilirubin,histidine,oleic acid,citrate,vanillin can be used as potential biomarkers to distinguish them.The pathways include ABC transporters,aminoacyl-tRNA biosynthesis,central carbon metabolism in cancer and so on.Conclusion:There are significant differences in the plasma metabolic components of UA and AMI.Studies related to metabolomics may guide significance for exploring the biological nature of ACS.
