OBJECTIVE Psoriasis is an immune system meditated disease,especially T cells.It disturbed many people around the world and hard to therapy.Paeonia lactiflora Pall has been used as a medicine in china for thousands of ...OBJECTIVE Psoriasis is an immune system meditated disease,especially T cells.It disturbed many people around the world and hard to therapy.Paeonia lactiflora Pall has been used as a medicine in china for thousands of years.Recent studies has found that the main component of Paeonia lactiflora Pall can alleviates the immune response in many diseases.In this study,we researched the effects and possible mechanisms of total glucosides of paeony(TGP)on animal psoriasis in order to study the therapeutic effects and mechanisms of TGP in 5%propranolol creaminduced psoriasis in guinea pigs and Imiquimod(IMQ)cream-induced psoriasis in mice.METHODS The effect of TGP was evaluated using a psoriasis-like model of guinea pigs and mice.Ear thickness was accessed,and pathology injury was observed by HE staining.The levels of serum IL-1β,IL-6,IL-12,IL-17,IL-23,TNF-α,and IFN-γ,skin IL-17A,IL-22 and orphan nuclear receptor(RORγt)mRNA expression,proliferating cell nuclear antigen(PCNA),total or phosphorylated signal transducers and activators of transcription(STAT1 and STAT3)were determined by ELISA,real time PCR,immu⁃nohistochemical staining,and Western blotting,respectively.RESULTS Compared with model group,TGP treatment decreased the ear thickness,improved pathology of psoriasis,alleviated IMQ-induced keratinocyte proliferation,reduced the inflammatory cytokine,and downregulated IL-17A,IL-22,and RORγt mRNA in mice.Further study indicated that TGP inhibited STAT1 and STAT3 phosphorylation in lesion skins of psoriasis-like mice.CONCLUSION TGP alleviates the symptoms of psoriasis-like guinea pigs and mice,and the possible mechanism may relate to inhibit T helper 17(TH17)cell differentiation and keratinocytes proliferation by inhibiting STAT1 and STAT3 phosphorylation.展开更多
Based on the fact that toughness degradation occurred in 1Cr21Ni5Ti duplex stainless steel during slow cooling in a temperature interval of 400-600 ℃ following brazed welding, a simulated embrittlement treatment was ...Based on the fact that toughness degradation occurred in 1Cr21Ni5Ti duplex stainless steel during slow cooling in a temperature interval of 400-600 ℃ following brazed welding, a simulated embrittlement treatment was utilized to evaluate its emhrittlement tendency. Accumulated experiences have shown that high Ti and A1 contents led frequently to severe toughness deterioration. To elucidate this phenomenon, four heats with various Ti and Al contents were prepared in laboratory-scale melts, which were used to investigate the inherent embrittlement nature. The results have shown that 550 ℃ aging led only to a modest toughness reduction, whereas, aging around 475 ℃ resulted in ferrite embrittlement, regardless of Ti, A1 levels. However, high Ti, A1 levels reduced the austenite phase, which, in the case of its inferior shape and distribution due to insufficient hot deformation, failed to resist cleavagetype cracks initiated in the ferrite phase. Increasing hot-forged areas reduction could modify the shape and distribution of austenite, resulting in the enhanced resistance of the austenite to the cleavage-type cracks, which have been confirmed to be beneficial for brittlement suppression of 1Cr21Ni5Ti duplex stainless steel.展开更多
基金China Pharmaceutical University "Double First-Class" University project(CPU2018GY32)National Science and Technology Major Project of China(2016ZX09101031)
文摘OBJECTIVE Psoriasis is an immune system meditated disease,especially T cells.It disturbed many people around the world and hard to therapy.Paeonia lactiflora Pall has been used as a medicine in china for thousands of years.Recent studies has found that the main component of Paeonia lactiflora Pall can alleviates the immune response in many diseases.In this study,we researched the effects and possible mechanisms of total glucosides of paeony(TGP)on animal psoriasis in order to study the therapeutic effects and mechanisms of TGP in 5%propranolol creaminduced psoriasis in guinea pigs and Imiquimod(IMQ)cream-induced psoriasis in mice.METHODS The effect of TGP was evaluated using a psoriasis-like model of guinea pigs and mice.Ear thickness was accessed,and pathology injury was observed by HE staining.The levels of serum IL-1β,IL-6,IL-12,IL-17,IL-23,TNF-α,and IFN-γ,skin IL-17A,IL-22 and orphan nuclear receptor(RORγt)mRNA expression,proliferating cell nuclear antigen(PCNA),total or phosphorylated signal transducers and activators of transcription(STAT1 and STAT3)were determined by ELISA,real time PCR,immu⁃nohistochemical staining,and Western blotting,respectively.RESULTS Compared with model group,TGP treatment decreased the ear thickness,improved pathology of psoriasis,alleviated IMQ-induced keratinocyte proliferation,reduced the inflammatory cytokine,and downregulated IL-17A,IL-22,and RORγt mRNA in mice.Further study indicated that TGP inhibited STAT1 and STAT3 phosphorylation in lesion skins of psoriasis-like mice.CONCLUSION TGP alleviates the symptoms of psoriasis-like guinea pigs and mice,and the possible mechanism may relate to inhibit T helper 17(TH17)cell differentiation and keratinocytes proliferation by inhibiting STAT1 and STAT3 phosphorylation.
文摘Based on the fact that toughness degradation occurred in 1Cr21Ni5Ti duplex stainless steel during slow cooling in a temperature interval of 400-600 ℃ following brazed welding, a simulated embrittlement treatment was utilized to evaluate its emhrittlement tendency. Accumulated experiences have shown that high Ti and A1 contents led frequently to severe toughness deterioration. To elucidate this phenomenon, four heats with various Ti and Al contents were prepared in laboratory-scale melts, which were used to investigate the inherent embrittlement nature. The results have shown that 550 ℃ aging led only to a modest toughness reduction, whereas, aging around 475 ℃ resulted in ferrite embrittlement, regardless of Ti, A1 levels. However, high Ti, A1 levels reduced the austenite phase, which, in the case of its inferior shape and distribution due to insufficient hot deformation, failed to resist cleavagetype cracks initiated in the ferrite phase. Increasing hot-forged areas reduction could modify the shape and distribution of austenite, resulting in the enhanced resistance of the austenite to the cleavage-type cracks, which have been confirmed to be beneficial for brittlement suppression of 1Cr21Ni5Ti duplex stainless steel.
