目的总结应用寰椎椎弓根提拉螺钉结合枢椎椎弓根螺钉行后路提拉复位固定植骨融合术治疗寰枢椎脱位的疗效。方法2010年1月一2014年12月本院共收治27例寰枢椎脱位患者，均有不同程度的枕颈部疼痛和活动受限，并伴有神经功能障碍，美国脊...目的总结应用寰椎椎弓根提拉螺钉结合枢椎椎弓根螺钉行后路提拉复位固定植骨融合术治疗寰枢椎脱位的疗效。方法2010年1月一2014年12月本院共收治27例寰枢椎脱位患者，均有不同程度的枕颈部疼痛和活动受限，并伴有神经功能障碍，美国脊髓损伤协会(ASIA)分级：B级2例，C级17例，D级8例； 日本骨科学会(J0 A )评分4~14分，平均8.3分。MRI示20例患者有不同程度的脊髓受压，其中8例脊髓受压节段髓内出现T2加权像高信号改z变。患者均为寰椎前脱位，术前均进行颅骨牵引，17例部分复位，10例不可复位。术前寰齿间距(A D I)4~15 mm，平均10.3 m m ;颈髓延髓角（C M A )im 0~135.70, 平均120.9〇〇均采用寰椎椎弓根提拉螺钉结合枢椎椎弓根螺钉行后路提拉复位固定植骨融合术，观察患者术后临床症状和神经功能改善情况及寰枢椎复位和植骨融合情况。结果所有患者均顺利完成手术，术中均未发生椎动脉和脊髓损伤。患者随访6~36个月，平均20个月。术后CT及MRI示寰枢椎序列重建满意，齿突区域脑脊液线清晰，脊髓无压迫。术后6个月随访时患者神经功能明显改善，2例B级患者提高至C级；17例C级患者中2 例提高至E 级，15例提高至D级；8例D级患者均提高至E 级。J0A评分10~17分，平均14.6分，平均改善率78.4%。术后ADI 2~4 m m ,平均2.6 mm; CMA139.2。~152.4。，平均144.6。。术后6个月随访时所有患者获得骨性融合；随访期间未发现螺钉松动、移位和断裂及寰枢椎再移位、失稳现象。结论寰枢椎脱位会造成寰枢椎不稳及脊髓受压，应用寰椎椎弓根提拉螺钉结合枢椎椎弓根螺钉后路提拉复位技术治疗可获得良好的临床效果。展开更多
China is a large marine country. Developing marine economy is an effective way to solve a series of problems with which man is faced, such as the want of natural resources, space limitation, the environmental deterior...China is a large marine country. Developing marine economy is an effective way to solve a series of problems with which man is faced, such as the want of natural resources, space limitation, the environmental deterioration, etc. This article analyzes the rich resources of marine biology, harbor, offshore oil and natural gas and coastal tourism resources in China and describes the developing features and regional differences of marine economy. To realize the sustainable development of marine economy in China, what we need to do are as follows: 1) to list exploiting ocean into national development strategy; 2) to realize integrated economy of sea and land; 3) to develop ocean by science and technology; 4) to perfect legal institution of marine environment; 5) to establish new idea of sea defending.展开更多
A new avirulent, heat-resistance HB92 strain of newcastle Disease Virus (NDV) was acquired from Australia V4 strain. Its complete nucleotides sequence was first determined. The entire genome of NDV HB92 consists of 15...A new avirulent, heat-resistance HB92 strain of newcastle Disease Virus (NDV) was acquired from Australia V4 strain. Its complete nucleotides sequence was first determined. The entire genome of NDV HB92 consists of 15 186 nucleotides (GenBank accession no. AY225110). It was demonstrated by sequence analysis that nucleotides homology of HB92 strain with virulent strain ZJ1 were 83.9%, and the homology compared with avirulent vaccine strain La Sota and B1 were 94.0% and 93.5%, respectively. These results might be contributive to the study of the molecular mechanism of evolution of the NDV strain HB92 and to develop the engineered recombinant vaccine. Key words newcastle disease virus - genomic sequence - sequence analysis CLC number S 852. 65 Foundation item: Supported by Hubei Natural Science Foundation (2002AB144)Biography: Pan Zhi-shu(1961-), male, Ph. D, Associate professor, research direction: molecular biology and pathogenesis of eucaryotic viruses.展开更多
To prepare 125/131I-β-CIT (2β-carbomethoxy-β- (4-iodophenyl)tropane) as an imaging agent for dopamine transporter (DAT), the labeling method from tributylstannyl precursor with peracetic acid has been reported in t...To prepare 125/131I-β-CIT (2β-carbomethoxy-β- (4-iodophenyl)tropane) as an imaging agent for dopamine transporter (DAT), the labeling method from tributylstannyl precursor with peracetic acid has been reported in this article. The radio-chemical purity (RCP) of the labeled compound was over 95% determined by HPLC and TLC. The stability, partition coefficients were also determined. The pharmacological studies of the imaging agent were performed in rats, mice, rabbits and normal monkey. The ligand showed preferable uptake in brain (1 .9%ID/organ in rats and 4.5%ID/organ in mice at 5 min). The ratios of striatum/cerebellum, hippocampus/cerebellum and cortex/cerebellum were 28.9, 3.97 and 4.75 at 6 h in rats, and 8.52, 2.99 and 3.06 at 6h in mice, respectively. In monkey brain imaging the ratios of striatum/frontal cortex (ST/FC) and striatum/occipital cortex (ST/OC) were 5.14 and 5.97 at 4 h, respectively. All of above showed the high affinity of the ligand to DAT. The compound was primarily metabo lized in liver because the hepatic uptake was much higher than other organs (75.4%ID/organ at 18h). The half-life of blood elimination was 5min The dose received by mice was 2500 times as high as that received by human in the test of undue toxicity, which evaluated the safety of the agent. All the results suggest that fl-CIT can be used as a potential DAT imaging agent.展开更多
Objective: To study effects of adenosine 5‘-triphosphate (ATP) on cochlear function of guinea pig. Methods: After perfusion of ATP into pcrilymphatic spaces of the guinea pig cochlea, summating potential (SP) , cochl...Objective: To study effects of adenosine 5‘-triphosphate (ATP) on cochlear function of guinea pig. Methods: After perfusion of ATP into pcrilymphatic spaces of the guinea pig cochlea, summating potential (SP) , cochlear microphonic ( CM ) , auditory nerve compound action potential (CAP), distortion product otoacoustic emission (DPOAE) and auditory brainstem response (ABR) were measured. Results: The results showed concentration-dependent effect of ATP on the response alterations of hioelectric activity in cochlea.Administration of lmmol/L ATP caused an increase both in the amplitude of the SP and in the threshold of ABR, a decrease in amplitude of the CAP and DPOAE. In addition, response alterations of the CAP and DPOAE showed in an intensity- and frequency-dependent manner, respectively. At levels of 20 -70dB nHL sound intensity, 1 mmol/L ATP caused a significant decrease in the CAP amplitude, while at moderate and high frequency ranges of 2 -8kHz it reduced DPOAE amplitude significantly. 330μmol/L ATP also increased the threshold of ABR. Conclusion: ATP through perilymphatic perfusion could inhibit cochlear function of guinea pig.展开更多
Background Macrophage migration inhibitory factor (MIF) plays a pivotal role in inflammatory and immune-mediated diseases. However, its molecular function and role in gastrointestinal diseases has rarely been studied ...Background Macrophage migration inhibitory factor (MIF) plays a pivotal role in inflammatory and immune-mediated diseases. However, its molecular function and role in gastrointestinal diseases has rarely been studied and thus warrants an in-depth investigation. This study was designed and conducted to determine MIF expression in Helicobacter pylori (H. pylori)-induced gastritis and the effect of H. pylori on MIF expression in monocytes in vitro. Methods Gastric specimens of 62 patients with chronic gastritis were obtained through endoscopic biopsies. Both gastric antrum and body were examined for histopathologic changes. Positive H. pylori was determined through rapid urease test and histopathological examination. A patient was classified as H. pylori positive if both tests showed positive results. The updated Sydney System was employed to assess the severity and activity of gastric inflammation. Double immunoassaying for MIF/T-cells (CD45RO) and MIF/macrophage (KP1), as well as in situ hybridization for the expression of MIF mRNA were used for the current analysis. THP-1, a monocyte cell line, was co-incubated with H. pylori strains (ATCC26695) and subsequently examined for the expression of MIF protein and mRNA by enzyme linked immunosorbent assay and retrospective transcription-polymerase chain reaction, respectively. Results Among 62 patients with chronic gastritis, significant increase in total T-cells, MIF+ T-cells, total macrophages, MIF+ macrophages and MIF mRNA+ cells was observed in 42 H. pylori positive patients compared to H. pylori negative patients. Moreover, the increase of the MIF mRNA+ cells was highly correlated with the severity of the disease(number of MIF mRNA+cells/mm2, mild: 2834±382, moderate: 3569±123, severe: 3881±118, P<0.01). In vitro results showed that the expression of MIF protein and mRNA in monocytes was significantly increased after incubation with H. pylori strains.Conclusions Overexpression of MIF is common in H. pylori-induced gastric inflammation, which suggests MIF may play an important role in the initiation and development of this disease.展开更多
Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the sensitivity of chemotherapy and radiotherapy. E1A have the ability to integrate into the host genome, resulting in long-time ex...Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the sensitivity of chemotherapy and radiotherapy. E1A have the ability to integrate into the host genome, resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization. This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy. Thus, we firstly constructed E1A eu-caryotic expression vector (pPIC9/E1A), transformated the pichia pastoris yeast cells (GS115) and screened the high-expressing recombinant strains. The positive yeast strains were cultured in the shake flask, and induced for 3 d. The crude E1A protein was purified using two steps of col-umn chromatography on HiTrap Q and HiTrap SP. The pu-rified E1A protein was identified by SDS-PAGE and Western blot. E1A protein was mostly located at cellular nuclear when Chariot delivered E1A protein into cells. The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase, and significantly inhibited the growth of LN686 tumor cells. The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.展开更多
The transcription factor nuclear factor kB (NF-kB) plays a key role in the delayed xenograft rejection (DXR). One of the important objects in the field is how to inhibit the NF-kB activity at optimal level. Thus, a mo...The transcription factor nuclear factor kB (NF-kB) plays a key role in the delayed xenograft rejection (DXR). One of the important objects in the field is how to inhibit the NF-kB activity at optimal level. Thus, a modified E1A gene (E1AD) containing function domain (1—80 aa) and nuclear localization domain (139—243 aa) was used and cloned into an eucaryotic expression vector pcDNA3 to transfect the porcine aortic endothelial cells (PAEC). The stable transfectants were screened with G418. E1AD gene was able to be stably expressed in the PAEC and could not affect the growth of PAEC as analyzed by RT-PCR and cell growth rate. Reporter gene assay demonstrated that E1AD was capable of inhibiting NF-kB activity in the PAEC in-duced by TNF-a without sensitizing to apoptosis, and the rate of inhibition was 53%. Furthermore, E1AD inhibited the expression of a NF-kBdependent inflammatory gene E-selectin in the cells, and the rate of inhibition was 63%. In summary, the usage of E1AD gene may be a new strategy to overcome DXR in the xenotransplantation.展开更多
文摘目的总结应用寰椎椎弓根提拉螺钉结合枢椎椎弓根螺钉行后路提拉复位固定植骨融合术治疗寰枢椎脱位的疗效。方法2010年1月一2014年12月本院共收治27例寰枢椎脱位患者，均有不同程度的枕颈部疼痛和活动受限，并伴有神经功能障碍，美国脊髓损伤协会(ASIA)分级：B级2例，C级17例，D级8例； 日本骨科学会(J0 A )评分4~14分，平均8.3分。MRI示20例患者有不同程度的脊髓受压，其中8例脊髓受压节段髓内出现T2加权像高信号改z变。患者均为寰椎前脱位，术前均进行颅骨牵引，17例部分复位，10例不可复位。术前寰齿间距(A D I)4~15 mm，平均10.3 m m ;颈髓延髓角（C M A )im 0~135.70, 平均120.9〇〇均采用寰椎椎弓根提拉螺钉结合枢椎椎弓根螺钉行后路提拉复位固定植骨融合术，观察患者术后临床症状和神经功能改善情况及寰枢椎复位和植骨融合情况。结果所有患者均顺利完成手术，术中均未发生椎动脉和脊髓损伤。患者随访6~36个月，平均20个月。术后CT及MRI示寰枢椎序列重建满意，齿突区域脑脊液线清晰，脊髓无压迫。术后6个月随访时患者神经功能明显改善，2例B级患者提高至C级；17例C级患者中2 例提高至E 级，15例提高至D级；8例D级患者均提高至E 级。J0A评分10~17分，平均14.6分，平均改善率78.4%。术后ADI 2~4 m m ,平均2.6 mm; CMA139.2。~152.4。，平均144.6。。术后6个月随访时所有患者获得骨性融合；随访期间未发现螺钉松动、移位和断裂及寰枢椎再移位、失稳现象。结论寰枢椎脱位会造成寰枢椎不稳及脊髓受压，应用寰椎椎弓根提拉螺钉结合枢椎椎弓根螺钉后路提拉复位技术治疗可获得良好的临床效果。
文摘China is a large marine country. Developing marine economy is an effective way to solve a series of problems with which man is faced, such as the want of natural resources, space limitation, the environmental deterioration, etc. This article analyzes the rich resources of marine biology, harbor, offshore oil and natural gas and coastal tourism resources in China and describes the developing features and regional differences of marine economy. To realize the sustainable development of marine economy in China, what we need to do are as follows: 1) to list exploiting ocean into national development strategy; 2) to realize integrated economy of sea and land; 3) to develop ocean by science and technology; 4) to perfect legal institution of marine environment; 5) to establish new idea of sea defending.
文摘A new avirulent, heat-resistance HB92 strain of newcastle Disease Virus (NDV) was acquired from Australia V4 strain. Its complete nucleotides sequence was first determined. The entire genome of NDV HB92 consists of 15 186 nucleotides (GenBank accession no. AY225110). It was demonstrated by sequence analysis that nucleotides homology of HB92 strain with virulent strain ZJ1 were 83.9%, and the homology compared with avirulent vaccine strain La Sota and B1 were 94.0% and 93.5%, respectively. These results might be contributive to the study of the molecular mechanism of evolution of the NDV strain HB92 and to develop the engineered recombinant vaccine. Key words newcastle disease virus - genomic sequence - sequence analysis CLC number S 852. 65 Foundation item: Supported by Hubei Natural Science Foundation (2002AB144)Biography: Pan Zhi-shu(1961-), male, Ph. D, Associate professor, research direction: molecular biology and pathogenesis of eucaryotic viruses.
基金Supported by the National Natural Science Foundation of China (39770230), Science Foundation of Health Ministry (98-1-326) and H
文摘To prepare 125/131I-β-CIT (2β-carbomethoxy-β- (4-iodophenyl)tropane) as an imaging agent for dopamine transporter (DAT), the labeling method from tributylstannyl precursor with peracetic acid has been reported in this article. The radio-chemical purity (RCP) of the labeled compound was over 95% determined by HPLC and TLC. The stability, partition coefficients were also determined. The pharmacological studies of the imaging agent were performed in rats, mice, rabbits and normal monkey. The ligand showed preferable uptake in brain (1 .9%ID/organ in rats and 4.5%ID/organ in mice at 5 min). The ratios of striatum/cerebellum, hippocampus/cerebellum and cortex/cerebellum were 28.9, 3.97 and 4.75 at 6 h in rats, and 8.52, 2.99 and 3.06 at 6h in mice, respectively. In monkey brain imaging the ratios of striatum/frontal cortex (ST/FC) and striatum/occipital cortex (ST/OC) were 5.14 and 5.97 at 4 h, respectively. All of above showed the high affinity of the ligand to DAT. The compound was primarily metabo lized in liver because the hepatic uptake was much higher than other organs (75.4%ID/organ at 18h). The half-life of blood elimination was 5min The dose received by mice was 2500 times as high as that received by human in the test of undue toxicity, which evaluated the safety of the agent. All the results suggest that fl-CIT can be used as a potential DAT imaging agent.
文摘Objective: To study effects of adenosine 5‘-triphosphate (ATP) on cochlear function of guinea pig. Methods: After perfusion of ATP into pcrilymphatic spaces of the guinea pig cochlea, summating potential (SP) , cochlear microphonic ( CM ) , auditory nerve compound action potential (CAP), distortion product otoacoustic emission (DPOAE) and auditory brainstem response (ABR) were measured. Results: The results showed concentration-dependent effect of ATP on the response alterations of hioelectric activity in cochlea.Administration of lmmol/L ATP caused an increase both in the amplitude of the SP and in the threshold of ABR, a decrease in amplitude of the CAP and DPOAE. In addition, response alterations of the CAP and DPOAE showed in an intensity- and frequency-dependent manner, respectively. At levels of 20 -70dB nHL sound intensity, 1 mmol/L ATP caused a significant decrease in the CAP amplitude, while at moderate and high frequency ranges of 2 -8kHz it reduced DPOAE amplitude significantly. 330μmol/L ATP also increased the threshold of ABR. Conclusion: ATP through perilymphatic perfusion could inhibit cochlear function of guinea pig.
文摘Background Macrophage migration inhibitory factor (MIF) plays a pivotal role in inflammatory and immune-mediated diseases. However, its molecular function and role in gastrointestinal diseases has rarely been studied and thus warrants an in-depth investigation. This study was designed and conducted to determine MIF expression in Helicobacter pylori (H. pylori)-induced gastritis and the effect of H. pylori on MIF expression in monocytes in vitro. Methods Gastric specimens of 62 patients with chronic gastritis were obtained through endoscopic biopsies. Both gastric antrum and body were examined for histopathologic changes. Positive H. pylori was determined through rapid urease test and histopathological examination. A patient was classified as H. pylori positive if both tests showed positive results. The updated Sydney System was employed to assess the severity and activity of gastric inflammation. Double immunoassaying for MIF/T-cells (CD45RO) and MIF/macrophage (KP1), as well as in situ hybridization for the expression of MIF mRNA were used for the current analysis. THP-1, a monocyte cell line, was co-incubated with H. pylori strains (ATCC26695) and subsequently examined for the expression of MIF protein and mRNA by enzyme linked immunosorbent assay and retrospective transcription-polymerase chain reaction, respectively. Results Among 62 patients with chronic gastritis, significant increase in total T-cells, MIF+ T-cells, total macrophages, MIF+ macrophages and MIF mRNA+ cells was observed in 42 H. pylori positive patients compared to H. pylori negative patients. Moreover, the increase of the MIF mRNA+ cells was highly correlated with the severity of the disease(number of MIF mRNA+cells/mm2, mild: 2834±382, moderate: 3569±123, severe: 3881±118, P<0.01). In vitro results showed that the expression of MIF protein and mRNA in monocytes was significantly increased after incubation with H. pylori strains.Conclusions Overexpression of MIF is common in H. pylori-induced gastric inflammation, which suggests MIF may play an important role in the initiation and development of this disease.
文摘Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the sensitivity of chemotherapy and radiotherapy. E1A have the ability to integrate into the host genome, resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization. This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy. Thus, we firstly constructed E1A eu-caryotic expression vector (pPIC9/E1A), transformated the pichia pastoris yeast cells (GS115) and screened the high-expressing recombinant strains. The positive yeast strains were cultured in the shake flask, and induced for 3 d. The crude E1A protein was purified using two steps of col-umn chromatography on HiTrap Q and HiTrap SP. The pu-rified E1A protein was identified by SDS-PAGE and Western blot. E1A protein was mostly located at cellular nuclear when Chariot delivered E1A protein into cells. The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase, and significantly inhibited the growth of LN686 tumor cells. The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.
基金This work was supported by Beijing "248"High-Tech Program China.
文摘The transcription factor nuclear factor kB (NF-kB) plays a key role in the delayed xenograft rejection (DXR). One of the important objects in the field is how to inhibit the NF-kB activity at optimal level. Thus, a modified E1A gene (E1AD) containing function domain (1—80 aa) and nuclear localization domain (139—243 aa) was used and cloned into an eucaryotic expression vector pcDNA3 to transfect the porcine aortic endothelial cells (PAEC). The stable transfectants were screened with G418. E1AD gene was able to be stably expressed in the PAEC and could not affect the growth of PAEC as analyzed by RT-PCR and cell growth rate. Reporter gene assay demonstrated that E1AD was capable of inhibiting NF-kB activity in the PAEC in-duced by TNF-a without sensitizing to apoptosis, and the rate of inhibition was 53%. Furthermore, E1AD inhibited the expression of a NF-kBdependent inflammatory gene E-selectin in the cells, and the rate of inhibition was 63%. In summary, the usage of E1AD gene may be a new strategy to overcome DXR in the xenotransplantation.
