Dear Editor, Nanosized particulate systems combining better cancer diagnosis with therapeutic effect are being designed based on the merging of nanotechnology with cellular and molecular techniques. The surface of the...Dear Editor, Nanosized particulate systems combining better cancer diagnosis with therapeutic effect are being designed based on the merging of nanotechnology with cellular and molecular techniques. The surface of these nanoscale carriers is often functionalized with biological molecules for stabilization and targeted delivery. The combinations of nano-core and associated functional molecules can cross the cell membrane [1], and the surface of nanomaterials （including coating and associated functional molecules） plays a critical role in determining the outcome of their interactions with cells [2, 3]. Studying the potential effects of nanomaterials in biological systems often requires the administration of nanoparticles into a cell culture system or into living organisms in vivo. It should be noted, however, that under such conditions nanopaticles are known to adsorb proteins from the biological system,展开更多
Hexagonal gallium nitride films were successfully fabricated throughammoniating Ga_2O_3 films deposited on silicon (111) substrates by electrophoresis. The structure,composition, and surface morphology of the formed f...Hexagonal gallium nitride films were successfully fabricated throughammoniating Ga_2O_3 films deposited on silicon (111) substrates by electrophoresis. The structure,composition, and surface morphology of the formed films were characterized by X-ray diffraction(XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and transmissionelectron microscopy (TEM). The measurement results reveal that the polycrystalline GaN films withhexagonal wurtzite structure were successfully grown on the silicon (111) substrates. Preliminaryresults suggest that varying the ammoniating temperature has obvious effect on the quality of theGaN films formed with this method.展开更多
dinitrotoluene(2,4 DNT), 2,6 dinitrotoluene(2,6 DNT) and 4 nitrotoluene(4 NT) are typical pollutants in the Songhua River of Northeast China. Sertoli/germ cell cocultures and single cell gel electrophoresis(SCGE) are ...dinitrotoluene(2,4 DNT), 2,6 dinitrotoluene(2,6 DNT) and 4 nitrotoluene(4 NT) are typical pollutants in the Songhua River of Northeast China. Sertoli/germ cell cocultures and single cell gel electrophoresis(SCGE) are applied to investigate whether they have genotoxicity on DNA damage of germ cell of Kunming male rat. The results showed that all three nitrotoluene compounds tested could induce DNA single strand breaks of the germ cell. A significant relationship is found between logarithm dose and the degree of DNA damage, which implies that 2,4 DNT, 2,6 DNT and 4 NT have genotoxicity and can induce the germ cell DNA strand to break in vitro.展开更多
The physical and thermal properties of P2O5-Al2O3-BaO-La2O3 glasses were investigated. The effects of glass compositions on the transition temperature, thermal expansion coefficient, density, hardness and refractive i...The physical and thermal properties of P2O5-Al2O3-BaO-La2O3 glasses were investigated. The effects of glass compositions on the transition temperature, thermal expansion coefficient, density, hardness and refractive index of glasses were studied. The highest hardness of the glasses is 4143.891 MPa and the lowest thermal expansion coefficient of the glasses is 71.770×10-7/℃. A phosphate glass with high mechanical strength and good thermal characteristic is obtained.展开更多
OBJECTIVE This study discusses the effects of scutellarin(SCU),which is one of effective component of Erigeron breviscapus(Vant.)Hand-Mazz,on cGMP dependent protein kinase(PKG)in human cardiac microvascular endothelia...OBJECTIVE This study discusses the effects of scutellarin(SCU),which is one of effective component of Erigeron breviscapus(Vant.)Hand-Mazz,on cGMP dependent protein kinase(PKG)in human cardiac microvascular endothelial cells(HCMECs)after hypoxia roxygenation(HR).METHODS Based on a model of HCMECs cells with HR injury,cell viability is examined by MTT assay.Protein expression of PKG is analyzed by Western blotting and its enzyme activity is investigated by ELISA technology.RESULTS The results of MTT assay- indicate that SCU(1,10μmol·L1)could protect HCMECs against HR injury.SCU(0.1,1 and 10μmol·L-1)canenhance PKG-I expression in control and HR injury cells.Furthermore,SCU(1,10μmol·L-1 significantly increase PKG activity in control cells(P<0.01),and also SCU(100μmol·L-1)appears similar on enzyme activity in HR injury cells(P <0.05).CONCLUSION SCU appears protective effects on endothelial cells against HR damage.While its mechanisms may be related to the influence of SCU on PKG activity in HCMECs.展开更多
OBJECTIVE To investigate the fast antidepressant effect and the underlying mechanism of it.METHODS C57BL/6 mice were exposed to a 6-week chronic unpredictable mild stress(CUMS)protocol,and treated with YY-23 or a posi...OBJECTIVE To investigate the fast antidepressant effect and the underlying mechanism of it.METHODS C57BL/6 mice were exposed to a 6-week chronic unpredictable mild stress(CUMS)protocol,and treated with YY-23 or a positive drug fluoxetine in the last 3weeks,behavioral assessments proceed every week.NMDA-induced current were recorded by whole-cell patch clamp on dissociated hippocampal CA1 neurons of Sprague-Dawley rats.Phosphorylation of the signaling proteins was evaluated by Western blotting.RESULTS The electrophysiological results showed that YY-23 is a non-competitive NMDA receptor antagonist,neither in′use-dependent′nor′voltage-dependent′way,with no prominent effect on AMPA induced current.This antagonism of YY-23 could be partially prevented by proven blockers MK801 and Mg2+,laterally indicating the nature of NMDA receptor antagonist both pharmacologically and physiologically.Behavioral and Biochemical study showed that YY-23 could act as antidepressant and exerted strong inhibitory effect on GSK3β in PFC of CMS depression mice model.CONCLUSION YY-23 showed promising antidepressant effect with rapid onset,which might be related to NMDA receptor antagonism and GSK3β inhibition.展开更多
A substituted polyacetylene bearing a furan ring, poly(2-propynyl 2-furoate) (PPF), was synthesized for the first time with palladium acetylide complex catalysts in CHCl3 at 60°C to give a soluble and stable poly...A substituted polyacetylene bearing a furan ring, poly(2-propynyl 2-furoate) (PPF), was synthesized for the first time with palladium acetylide complex catalysts in CHCl3 at 60°C to give a soluble and stable polymer with a yield of ca. 75%. Some features for polymerization of 2-propynyl 2-furoate are described and discussed. The resulting polymer was characterized by IR, GPC and 1H-NMR analysis.展开更多
Using chiral hydrogenesterification reaction as the key step, the stereoselective synthesisof (+)-nuciferal 1, (+)-(E)-nuciferol 2 and (+)-α-curcumene 3 has been achieved.
Activin A is a kind of pre-inflammatory factor that belongs to the transforming growth factor-β(TGF-β) superfamily.To investigate the effect and mechanism of activin A on the activities of mouse macrophages,the secr...Activin A is a kind of pre-inflammatory factor that belongs to the transforming growth factor-β(TGF-β) superfamily.To investigate the effect and mechanism of activin A on the activities of mouse macrophages,the secretion of NO in the supernatant of cultured mouse peritoneal macrophages was examined by NO assay kit,and the expression of iNOS,ActRIIA and ARIP2 mRNA in mouse peritoneal macrophages was analyzed by RT-PCR. The results showed that activin A stimulated the secretion of NO and the expression of iNOS mRNA in non-activated mouse macrophages in a time-and dose-dependent manner.In contrast,activin A in the same concentration inhibited the secretion of NO in LPS-activated mouse macrophages in a dose-dependent manner. ActRIIA was highly expressed on macrophages,and activin A upregulated the ActRIIA mRNA expression in macrophages.Anti-ActRIIA antibody can block the secretion of NO from the macrophages stimulated by activin A. Furthermore,RT-PCR analysis revealed that activin A enhanced the ARIP2 mRNA expression in macrophages. These results indicated that Activin A may be a weak activator compared with LPS to mouse macrophages,and activin A may modulate the secretion of NO through ActRIIA-ARIP2 signal pathway in mouse macrophages. Cellular & Molecular Immunology.2005;2(1):63-67.展开更多
文摘Dear Editor, Nanosized particulate systems combining better cancer diagnosis with therapeutic effect are being designed based on the merging of nanotechnology with cellular and molecular techniques. The surface of these nanoscale carriers is often functionalized with biological molecules for stabilization and targeted delivery. The combinations of nano-core and associated functional molecules can cross the cell membrane [1], and the surface of nanomaterials （including coating and associated functional molecules） plays a critical role in determining the outcome of their interactions with cells [2, 3]. Studying the potential effects of nanomaterials in biological systems often requires the administration of nanoparticles into a cell culture system or into living organisms in vivo. It should be noted, however, that under such conditions nanopaticles are known to adsorb proteins from the biological system,
基金This work is financially supported by the Key Research Program of National Natural Science Foundation of China (No. 90201025)the National Natural Science Foundation of China (No. 60071006)
文摘Hexagonal gallium nitride films were successfully fabricated throughammoniating Ga_2O_3 films deposited on silicon (111) substrates by electrophoresis. The structure,composition, and surface morphology of the formed films were characterized by X-ray diffraction(XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and transmissionelectron microscopy (TEM). The measurement results reveal that the polycrystalline GaN films withhexagonal wurtzite structure were successfully grown on the silicon (111) substrates. Preliminaryresults suggest that varying the ammoniating temperature has obvious effect on the quality of theGaN films formed with this method.
文摘dinitrotoluene(2,4 DNT), 2,6 dinitrotoluene(2,6 DNT) and 4 nitrotoluene(4 NT) are typical pollutants in the Songhua River of Northeast China. Sertoli/germ cell cocultures and single cell gel electrophoresis(SCGE) are applied to investigate whether they have genotoxicity on DNA damage of germ cell of Kunming male rat. The results showed that all three nitrotoluene compounds tested could induce DNA single strand breaks of the germ cell. A significant relationship is found between logarithm dose and the degree of DNA damage, which implies that 2,4 DNT, 2,6 DNT and 4 NT have genotoxicity and can induce the germ cell DNA strand to break in vitro.
基金This work was finicially supported by the National Natu-ral Science Foundation of China under grant No.69988006.
文摘The physical and thermal properties of P2O5-Al2O3-BaO-La2O3 glasses were investigated. The effects of glass compositions on the transition temperature, thermal expansion coefficient, density, hardness and refractive index of glasses were studied. The highest hardness of the glasses is 4143.891 MPa and the lowest thermal expansion coefficient of the glasses is 71.770×10-7/℃. A phosphate glass with high mechanical strength and good thermal characteristic is obtained.
基金The project supported by National Natural Science Foundation of China(30960450,81373964,81173110and 81402991)Yunnan Provincial Science and Technology Department(2011FA022,2014FA010,2014IA033and 2014BC012)+1 种基金Shanghai Science&Technology Support Program(13431900401)National Science&Technology Major Project of China(2014ZX09301-306-03)
文摘OBJECTIVE This study discusses the effects of scutellarin(SCU),which is one of effective component of Erigeron breviscapus(Vant.)Hand-Mazz,on cGMP dependent protein kinase(PKG)in human cardiac microvascular endothelial cells(HCMECs)after hypoxia roxygenation(HR).METHODS Based on a model of HCMECs cells with HR injury,cell viability is examined by MTT assay.Protein expression of PKG is analyzed by Western blotting and its enzyme activity is investigated by ELISA technology.RESULTS The results of MTT assay- indicate that SCU(1,10μmol·L1)could protect HCMECs against HR injury.SCU(0.1,1 and 10μmol·L-1)canenhance PKG-I expression in control and HR injury cells.Furthermore,SCU(1,10μmol·L-1 significantly increase PKG activity in control cells(P<0.01),and also SCU(100μmol·L-1)appears similar on enzyme activity in HR injury cells(P <0.05).CONCLUSION SCU appears protective effects on endothelial cells against HR damage.While its mechanisms may be related to the influence of SCU on PKG activity in HCMECs.
基金The project supported by the National Natural Science Foundation(31128009,31171011)the National Science&Technology Major Project″Key New Drug Creation and Manufacturing Program″(2014ZX09102-001-005)the Ministry of Science and Technology(2013CB910601)
文摘OBJECTIVE To investigate the fast antidepressant effect and the underlying mechanism of it.METHODS C57BL/6 mice were exposed to a 6-week chronic unpredictable mild stress(CUMS)protocol,and treated with YY-23 or a positive drug fluoxetine in the last 3weeks,behavioral assessments proceed every week.NMDA-induced current were recorded by whole-cell patch clamp on dissociated hippocampal CA1 neurons of Sprague-Dawley rats.Phosphorylation of the signaling proteins was evaluated by Western blotting.RESULTS The electrophysiological results showed that YY-23 is a non-competitive NMDA receptor antagonist,neither in′use-dependent′nor′voltage-dependent′way,with no prominent effect on AMPA induced current.This antagonism of YY-23 could be partially prevented by proven blockers MK801 and Mg2+,laterally indicating the nature of NMDA receptor antagonist both pharmacologically and physiologically.Behavioral and Biochemical study showed that YY-23 could act as antidepressant and exerted strong inhibitory effect on GSK3β in PFC of CMS depression mice model.CONCLUSION YY-23 showed promising antidepressant effect with rapid onset,which might be related to NMDA receptor antagonism and GSK3β inhibition.
基金This work was supported by the Scientific and Technological Agreement, China-France.
文摘A substituted polyacetylene bearing a furan ring, poly(2-propynyl 2-furoate) (PPF), was synthesized for the first time with palladium acetylide complex catalysts in CHCl3 at 60°C to give a soluble and stable polymer with a yield of ca. 75%. Some features for polymerization of 2-propynyl 2-furoate are described and discussed. The resulting polymer was characterized by IR, GPC and 1H-NMR analysis.
文摘Using chiral hydrogenesterification reaction as the key step, the stereoselective synthesisof (+)-nuciferal 1, (+)-(E)-nuciferol 2 and (+)-α-curcumene 3 has been achieved.
基金supported by the National Natural Science Foundation of China(No.30170478,30400400)International Cooperation Projects of Jilin Province of China(No.20040707-6).
文摘Activin A is a kind of pre-inflammatory factor that belongs to the transforming growth factor-β(TGF-β) superfamily.To investigate the effect and mechanism of activin A on the activities of mouse macrophages,the secretion of NO in the supernatant of cultured mouse peritoneal macrophages was examined by NO assay kit,and the expression of iNOS,ActRIIA and ARIP2 mRNA in mouse peritoneal macrophages was analyzed by RT-PCR. The results showed that activin A stimulated the secretion of NO and the expression of iNOS mRNA in non-activated mouse macrophages in a time-and dose-dependent manner.In contrast,activin A in the same concentration inhibited the secretion of NO in LPS-activated mouse macrophages in a dose-dependent manner. ActRIIA was highly expressed on macrophages,and activin A upregulated the ActRIIA mRNA expression in macrophages.Anti-ActRIIA antibody can block the secretion of NO from the macrophages stimulated by activin A. Furthermore,RT-PCR analysis revealed that activin A enhanced the ARIP2 mRNA expression in macrophages. These results indicated that Activin A may be a weak activator compared with LPS to mouse macrophages,and activin A may modulate the secretion of NO through ActRIIA-ARIP2 signal pathway in mouse macrophages. Cellular & Molecular Immunology.2005;2(1):63-67.
