There is a method for investigating the transformation of resistance gene of carbendazim into Trichoderma harzianum. In order to introduce the resistance to benzimidazole fungicide into bio-control microorganism Trich...There is a method for investigating the transformation of resistance gene of carbendazim into Trichoderma harzianum. In order to introduce the resistance to benzimidazole fungicide into bio-control microorganism Trichoderma harzianum was transformed with the resistance gene. In this study, we investigate resistance level and calculate EC 50 (effective concentration of carbendazim that can survive 50% of Trichoderma harzianum in that concentration) and stability of the resistance for the transformant isolate of Trichoderma harzianum. Results show the transformants can growth on the medium containing more than 1 000 μg/ml carbendazim and the resistance is stabled after 10 times transfer on non-selective medium and have EC 50 average about, 1 200 μg/ml.展开更多
An about 1.40 Kb target gene fragment was yielded by PCR amplification with the plasmid pRB 129,which was identified by restriction enzyme digestion that the PCR product was TUB2 gene.The gene was digested by the rest...An about 1.40 Kb target gene fragment was yielded by PCR amplification with the plasmid pRB 129,which was identified by restriction enzyme digestion that the PCR product was TUB2 gene.The gene was digested by the restriction enzyme and was linked with pTA plasmid to construct pTA TUB2 plasmid.The plasmid was transformed into Chaetomium spp.by PEG method and the transformation rate was 27/(2×10 5) and it is nine times higher than that of pRB 129.The transformants can grow on the PDA containing 1 000 μg·mL -1 carbendazim,which is 1 000 times higher than the original Chaetomium spp.The resistance was stable after 10 times transfer on non selective medium.展开更多
The article explores the effectiveness of story writing in L2 teaching. A language teaching method based on the theory of language acquisition and focuses on students for active and meaningful learning is essential fo...The article explores the effectiveness of story writing in L2 teaching. A language teaching method based on the theory of language acquisition and focuses on students for active and meaningful learning is essential for EFL students. Free story writing, with its unique advantages, motivates and strengthens students' learning and improves students' writing ability by creating a strong context for the target language. The paper discusses the topic by examining the writing process, story principles, composition, and second language acquisition including Input Hypothesis and Affective Filter Hypothesis.展开更多
文摘There is a method for investigating the transformation of resistance gene of carbendazim into Trichoderma harzianum. In order to introduce the resistance to benzimidazole fungicide into bio-control microorganism Trichoderma harzianum was transformed with the resistance gene. In this study, we investigate resistance level and calculate EC 50 (effective concentration of carbendazim that can survive 50% of Trichoderma harzianum in that concentration) and stability of the resistance for the transformant isolate of Trichoderma harzianum. Results show the transformants can growth on the medium containing more than 1 000 μg/ml carbendazim and the resistance is stabled after 10 times transfer on non-selective medium and have EC 50 average about, 1 200 μg/ml.
基金Heilongjiang Province Natural Science fund( Grant No.C0 2 0 3 )
文摘An about 1.40 Kb target gene fragment was yielded by PCR amplification with the plasmid pRB 129,which was identified by restriction enzyme digestion that the PCR product was TUB2 gene.The gene was digested by the restriction enzyme and was linked with pTA plasmid to construct pTA TUB2 plasmid.The plasmid was transformed into Chaetomium spp.by PEG method and the transformation rate was 27/(2×10 5) and it is nine times higher than that of pRB 129.The transformants can grow on the PDA containing 1 000 μg·mL -1 carbendazim,which is 1 000 times higher than the original Chaetomium spp.The resistance was stable after 10 times transfer on non selective medium.
文摘The article explores the effectiveness of story writing in L2 teaching. A language teaching method based on the theory of language acquisition and focuses on students for active and meaningful learning is essential for EFL students. Free story writing, with its unique advantages, motivates and strengthens students' learning and improves students' writing ability by creating a strong context for the target language. The paper discusses the topic by examining the writing process, story principles, composition, and second language acquisition including Input Hypothesis and Affective Filter Hypothesis.
