基于PI3K/AKT/mTOR信号通路探讨大黄煎剂对轻微型肝性脑病大鼠脑组织炎症损伤的保护机制

目的 探讨大黄煎剂保留灌肠对轻微型肝性脑病(MHE)大鼠模型脑组织炎症损伤的改善作用及可能机制。方法 60只SD雄性大鼠按完全随机方法分为空白组(CON组,n=6)和慢性肝硬化造模组(n=54)。12周后慢性肝硬化造模成功并经Morris水迷宫测试确认符合MHE模型大鼠40只,采用完全随机方法分为模型组(MOD组,n=8)、乳果糖组(LT组,n=8)、大黄煎剂低剂量组(RD1组,n=8)、大黄煎剂中剂量组(RD2组,n=8)和大黄煎剂高剂量组(RD3组,n=8)。其中CON组和MOD组大鼠用生理盐水保留灌肠,2 mL/只,1次/d;LT组大鼠用乳果糖按22.5%剂量保留灌肠,2 mL/只,1次/d;RD1组、RD2组、RD3组大鼠分别用大黄煎剂按2.5、5.0、7.5 g/kg三种剂量保留灌肠,2 mL/只,1次/d。所有大鼠治疗10 d后,进行Morris水迷宫测试,分析大鼠的空间学习记忆能力。分析大鼠行为学状态;检测大鼠血清ALT、AST、IL-1β、IL-6、TNF-α和血氨水平;观察大鼠肝组织和脑组织病理学变化;检测大鼠脑组织磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(AKT)、雷帕霉素靶蛋白(mTOR)的mRNA和蛋白表达水平。计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果 与MOD组比较,RD1组、RD2组和RD3组大鼠逃避潜伏期时间显著缩短(P值均<0.01),ALT、AST、IL-1β、IL-6、TNF-α和血氨水平明显降低(P值均<0.05),肝细胞、脑细胞变性、坏死和炎症程度减轻,脑组织PI3K、AKT、mTOR的mRNA和蛋白表达水平均降低(P值均<0.05),且RD3组治疗效果优于RD1组和RD2组。结论 大黄煎剂保留灌肠能够改善MHE大鼠认知功能及脑组织炎症损伤,其作用机制可能与调控PI3K/AKT/mTOR信号通路有关。

Image Inpainting Technique Incorporating Edge Prior and Attention Mechanism

Recently,deep learning-based image inpainting methods have made great strides in reconstructing damaged regions.However,these methods often struggle to produce satisfactory results when dealing with missing images with large holes,leading to distortions in the structure and blurring of textures.To address these problems,we combine the advantages of transformers and convolutions to propose an image inpainting method that incorporates edge priors and attention mechanisms.The proposed method aims to improve the results of inpainting large holes in images by enhancing the accuracy of structure restoration and the ability to recover texture details.This method divides the inpainting task into two phases:edge prediction and image inpainting.Specifically,in the edge prediction phase,a transformer architecture is designed to combine axial attention with standard self-attention.This design enhances the extraction capability of global structural features and location awareness.It also balances the complexity of self-attention operations,resulting in accurate prediction of the edge structure in the defective region.In the image inpainting phase,a multi-scale fusion attention module is introduced.This module makes full use of multi-level distant features and enhances local pixel continuity,thereby significantly improving the quality of image inpainting.To evaluate the performance of our method.comparative experiments are conducted on several datasets,including CelebA,Places2,and Facade.Quantitative experiments show that our method outperforms the other mainstream methods.Specifically,it improves Peak Signal-to-Noise Ratio(PSNR)and Structure Similarity Index Measure(SSIM)by 1.141~3.234 db and 0.083~0.235,respectively.Moreover,it reduces Learning Perceptual Image Patch Similarity(LPIPS)and Mean Absolute Error(MAE)by 0.0347~0.1753 and 0.0104~0.0402,respectively.Qualitative experiments reveal that our method excels at reconstructing images with complete structural information and clear texture details.Furthermore,our model exhibits impressive performance in terms of the number of parameters,memory cost,and testing time.

基于灾害空间信息的MR安全救援路线指引系统

为了协助救援人员在灾害救援过程中,更好地掌握灾害环境信息以及更快地开展救援工作,设计了一种基于灾害空间信息的MR安全救援路线指引系统,系统通过采集并处理灾害空间信息,实现了MR灾害空间信息可视化以及安全救援路线指引的功能。根据系统的软硬件需求,设计了对灾害场景中物理场分布信息获取的方案、最佳救援路线的生成、混合现实三维可视化的方法以及体感辅助温度手环的实现。帮助救援人员掌握灾害空间信息,提供安全救援路线指引,为现场救援工作提供引导,进一步降低救灾成本,减少人员伤亡。

基于加权基因共表达网络分析及生物信息学分析鉴定肝细胞癌临床特征关键基因

目的:通过加权基因共表达网络分析鉴定肝细胞癌(hepatocellular carcinoma,HCC)临床特征密切相关模块基因,为临床早期诊断及治疗提供参考。方法:从GEO数据库下载GSE84598芯片数据,综合加权基因共表达网络分析提取与HCC临床特征密切相关的模块基因。通过最大集团中心性(maximal clique centrality,MCC)算法对蛋白相互作用网络分析,鉴定出枢纽基因;最后通过TCGA数据库验证枢纽基因的表达情况、Kaplan-Meier Plotter在线数据库评估枢纽基因与HCC患者的预后关系。结果:通过对比HCC组织样本与正常肝组织样本的基因表达数据,共获得6262个差异表达基因,其中上调2207个,下调4055个。运用加权基因共表达网络分析鉴定出关键模块的120个基因;通过与差异表达基因取交集,得到候选枢纽基因115个。富集分析结果显示,候选枢纽基因与细胞有丝分裂、p53信号通路等密切相关。进一步运用MCC算法对115个候选枢纽基因的蛋白相互作用网络进行分析,鉴定出5个枢纽基因,即NUF2、RRM2、UBE2C、CDC20和MAD2L1。通过TCGA数据库对枢纽基因的验证,结果发现,与正常肝组织相比,在HCC组织中5个枢纽基因均明显上调;而且生存分析显示枢纽基因的高表达与HCC患者的不良预后密切相关。结论:本研究通过结合多个数据库鉴定出了5个枢纽基因,为HCC的临床诊断及治疗提供方向。

Deep learning assisted variational Hilbert quantitative phase imaging

We propose a high-accuracy artifacts-free single-frame digital holographic phase demodulation scheme for relatively lowcarrier frequency holograms-deep learning assisted variational Hilbert quantitative phase imaging(DL-VHQPI).The method,incorporating a conventional deep neural network into a complete physical model utilizing the idea of residual compensation,reliably and robustly recovers the quantitative phase information of the test objects.It can significantly alleviate spectrum-overlapping-caused phase artifacts under the slightly off-axis digital holographic system.Compared to the conventional end-to-end networks(without a physical model),the proposed method can reduce the dataset size dramatically while maintaining the imaging quality and model generalization.The DL-VHQPI is quantitatively studied by numerical simulation.The live-cell experiment is designed to demonstrate the method's practicality in biological research.The proposed idea of the deep learning-assisted physical model might be extended to diverse computational imaging techniques.

藏木水电站竖缝式鱼道过鱼效果初探

藏木水电站鱼道是少有的海拔高、长度长的竖缝式鱼道,其建设运行对上下游鱼类基因交流、种群繁衍尤为重要。为了解藏木鱼道过鱼效果,2021年3~7月采用搬罾抽样调查和视频观测结合的方法连续监测藏木鱼道过鱼种类、数量及其环境因子,共监测到鱼类9种143680尾,属2目4科7属,以设计主要过鱼对象异齿裂腹鱼、巨须裂腹鱼和拉萨裂腹鱼为主,并表现出明显的季节节律和昼夜节律,过鱼数量和种类呈5、6月份显著高于其他月份,白天明显高于夜间的特征。鱼道环境因子典范对应分析(CCA)结果表明,鱼道进出口水位和水温是影响过鱼效果的主要环境因子。研究结果可为藏木鱼道优化与有效运行提供数据支持,对雅鲁藏布江中下游渔业资源保护有重要意义。

不同覆膜处理对马铃薯田主要杂草的防除效果及安全性评价

【目的】明确不同覆膜方式对马铃薯田间杂草的防效以及对马铃薯生理指标、产量和商品薯率的影响。【方法】以马铃薯田主要杂草为研究对象,采用随机区组设计,研究白膜、白膜+330 g/L二甲戊灵、黑膜、黑膜+330 g/L二甲戊灵4种覆膜方式对马铃薯田间杂草的防效及对马铃薯的安全性。【结果】黑膜+330 g/L二甲戊灵处理对主要杂草的防效最好;达到95%以上,马铃薯的出苗率达到95.72%;折合产量达到2334.42 kg/667 m^(2),较对照增产24.30%;商品薯率达80.07%。【结论】黑膜+330 g/L二甲戊灵处理对田间主要杂草防除效果最好,建议推广应用。

新形势下中医药高层次人才培养的探索与实践

党的十八大以来,以习近平同志为核心的党中央把中医药工作摆在突出位置,中医药改革发展取得令人瞩目的重大成就。但目前中医药仍然一定程度存在高质量供给不够、人才总量不足、创新体系不完善、发展特色不突出等问题。我校坚持以中国特色社会主义思想为指导,全面贯彻落实党的方针政策,遵循中医药发展规律,破解存在的问题,以中医药为主,充分发挥壮瑶医药特色和优势,提高中医药人才培养质量,培养更多中医药高层次人才,为中医药事业发展提供科学依据。

New Interpretation of Neonatal Outcomes by Phenotypically Classified Preterm Syndrome:A Retrospective Cohort Study

Objective:The global aim to lower preterm birth rates has been hampered by the insufficient and incomplete understanding of its etiology,classification,and diagnosis.This study was designed to evaluate the association of phenotypically classified preterm syndromes with neonatal outcomes;to what extent would these outcomes be modified after the obstetric interventions,including use of glucocorticoid,magnesium sulfate,and progesterone.Methods:This was a retrospective cohort study conducted at Tongji Hospital(composed of Main Branch,Optical Valley Branch and Sino-French New City Branch)in Wuhan.A total of 900 pregnant women and 1064 neonates were retrospectively enrolled.The outcomes were the distribution of different phenotypes among parturition signs and pathway to delivery,the association of phenotypically classified clusters with short-term unfavorable neonatal outcomes,and to what extent these outcomes could be modified by obstetric interventions.Results:Eight clusters were identified using two-step cluster analysis,including premature rupture of fetal membranes(PPROM)phenotype,abnormal amniotic fluid(AF)phenotype,placenta previa phenotype,mixed condition phenotype,fetal distress phenotype,preeclampsia-eclampsia&hemolysis,elevated liver enzymes,and low platelets syndrome(PE-E&HELLP)phenotype,multiple fetus phenotype,and no main condition phenotype.Except for no main condition phenotype,the other phenotypes were associated with one or more complications,which conforms to the clinical practice.Compared with no main condition phenotype,some phenotypes were significantly associated with short-term adverse neonatal outcomes.Abnormal AF phenotype,mixed condition phenotype,PE-E&HELLP phenotype,and multiple fetus phenotype were risk factors for neonatal small-for gestation age(SGA);placenta previa phenotype was not associated with adverse outcomes except low APGAR score being 0-7 at one min;mixed condition phenotype was associated with low APGAR scores,SGA,mechanical ventilation,and gradeⅢ-Ⅳintraventricular hemorrhage(IVH);fetal distress phenotype was frequently associated with neonatal SGA and mechanical ventilation;PE-E&HELLP phenotype was correlated with low APGAR score being 0-7 at one min,SGA and neonatal intensive care unit(NICU)admission;multiple fetus phenotype was not a risk factor for the outcomes included except for SGA.Not all neonates benefited from obstetric interventions included in this study.Conclusion:Our research disclosed the independent risk of different preterm phenotypes for adverse pregnancy outcomes.This study is devoted to putting forward the paradigm of classifying preterm birth phenotypically,with the ultimate purpose of defining preterm phenotypes based on multi-center studies and diving into the underlying mechanisms.

Integrated analysis of comorbidity, pregnant outcomes, and amniotic fluid cytogenetics of fetuses with persistent left superior vena cava

BACKGROUND Persistent left superior vena cava(PLSVC)is the most common venous system variant.The clinical characteristics and amniotic fluid cytogenetics of fetuses with PLSVC remain to be further explored.AIM To develop reliable prenatal diagnostic recommendations through integrated analysis of the clinical characteristics of fetuses with PLSVC.METHODS Cases of PLSVC diagnosed using prenatal ultrasonography between September 2019 and November 2022 were retrospectively studied.The clinical characteristics of the pregnant women,ultrasonic imaging information,gestational age at diagnosis,pregnancy outcomes,and amniocentesis results were summarized and analyzed using categorical statistics and the chi-square test or Fisher’s exact test.RESULTS Of the 97 cases diagnosed by prenatal ultrasound,49(50.5%)had isolated PLSVC and 48(49.5%)had other structural abnormalities.The differences in pregnancy outcomes and amniocentesis conditions between the two groups were statistically significant(P<0.05).No significant differences were identified between the two groups in terms of advanced maternal age and gestational age(P>0.05).According to the results of the classification statistics,the most common intrac-ardiac abnormality was a ventricular septal defect and the most common extrac-ardiac abnormality was a single umbilical artery.In the subgroup analysis,the concurrent combination of intra-and extracardiac structural abnormalities was a risk factor for adverse pregnancy outcomes(odds ratio>1,P<0.05).Additional-ly,all abnormal cytogenetic findings on amniocentesis were observed in the comorbidity group.One case was diagnosed with 21-trisomy and six cases was diagnosed with chromosome segment duplication.CONCLUSION Examination for other structural abnormalities is strongly recommended when PLSVC is diagnosed.Poorer pregnancy outcomes and increased amniocentesis were observed in PLSVC cases with other structural abnor-malities.Amniotic fluid cytogenetics of fetuses is recommended for PLSVC with other structural abnormalities.

Identification of key genes underlying clinical features of hepatocellular carcinoma based on weighted gene co‑expression network analysis and bioinformatics analysis

Objective: To identify module genes that are closely related to clinical features of hepatocellular carcinoma (HCC) by weighted gene co‑expression network analysis, and to provide a reference for early clinical diagnosis and treatment. Methods: GSE84598 chip data were downloaded from the GEO database, and module genes closely related to the clinical features of HCC were extracted by comprehensive weighted gene co‑expression network analysis. Hub genes were identified through protein interaction network analysis by the maximum clique centrality (MCC) algorithm;Finally, the expression of hub genes was validated by TCGA database and the Kaplan Meier plotter online database was used to evaluate the prognostic relationship between hub genes and HCC patients. Results: By comparing the gene expression data between HCC tissue samples and normal liver tissue samples, a total of 6 262 differentially expressed genes were obtained, of which 2 207 were upregulated and 4 055 were downregulated. Weighted gene co‑expression network analysis was applied to identify 120 genes of key modules. By intersecting with the differentially expressed genes, 115 candidate hub genes were obtained. The results of enrichment analysis showed that the candidate hub genes were closely related to cell mitosis, p53 signaling pathway and so on. Further application of the MCC algorithm to the protein interaction network of 115 candidate hub genes identified five hub genes, namely NUF2, RRM2, UBE2C, CDC20 and MAD2L1. Validation of hub genes by TCGA database revealed that all five hub genes were significantly upregulated in HCC tissues compared to normal liver tissues;Moreover, survival analysis revealed that high expression of hub genes was closely associated with poor prognosis in HCC patients. Conclusions: This study identifies five hub genes by combining multiple databases, which may provide directions for the clinical diagnosis and treatment of HCC.

基于“瘀毒”与血脂异常相关病症的关系探讨慢性疾病转归:治未病理论的提出与思考

赵为民教授认为,慢性疾病迁延日久亦多瘀毒,即病久瘀血发展到一定程度会内生毒邪,或邪极生毒,或虚极生毒。基于对"瘀""毒"的认识,推测"瘀毒"是血脂异常的病理现象,亦是导致多种慢性疾病发生的共性病因病机,甚至为慢性疾病转归的重要因素。从中医证候的演变规律可以看出,"瘀毒"与慢性疾病关联,尤其与血脂异常及相关疾病关系密切。临床对于疑难杂症、危重症均从瘀毒论治,且无不效验。从"瘀毒"理论探讨其对慢性疾病转归的影响,可为中医辨证论治方法学的异病同治提供理论基础,同时从治未病角度可建立药物作用慢病的效应机制,抓住慢病治疗的时间节点,群体化服务慢病人群,对治未病理论进行补充。

硬件与混合现实结合的掘进机培训方案研究

传统的掘进机培训效率差,缺乏一种高效安全的培训方案。目前关于掘进机培训方案的研究多数仅基于虚拟现实技术,缺乏与其他技术结合使用的相关研究。为了提高学生培训质量,设计了一种具有评价系统和姿态识别系统,并且可通过物理硬件控制虚拟掘进机的培训方案。硬件控制部分主要设计了矩阵键盘和控制手柄模拟真实控制面板和操纵手柄,软件部分使用Maya对掘进机精细建模,使用C#编程语言编写逻辑脚本。姿态识别模块使用均值滤波对图像空间域降噪处理,再用帧间差分法进行目标检测,使用Open-Pose算法提取目标人物骨点信息,最终使用模板匹配法进行姿态识别。其结果表明:姿态识别、评分系统和硬件控制三者结合使用的掘进机培训方案可以有效规范学员操作姿态,提高培训质量。

基于虚拟现实的交通事故应急仿真培训系统

为了克服传统交通事故应急培训方式效果不佳的问题,设计了一种全新的应急仿真培训系统,其中包括交通事故过程的模拟、记录学员培训姿态信息以及培训结果的评分等功能。系统运用虚拟现实头显和手柄进行虚拟交互,基于Unreal Engine 4开发,使用Perception Neuron捕捉学院动作信息。为用户提供了高度沉浸式、实时性和稳定性的交通事故仿真培训体验。

针刺配合中药灌肠在妇科疾病中的临床应用

目的探讨针刺配合中药灌肠治疗妇科疾病的方法,为临床治疗妇科疾病提供科学理论依据。方法将子宫腺肌症所致的肾虚血瘀型痛经的72名患者随机分为对照组和观察组,各36例。采用中药保留灌肠进行治疗的为对照组,采用针刺配合中药保留灌肠进行治疗的观察组。对2组的临床疗效进行评估,观察治疗前后患者的相关指标的变化。结果对照组总有效率为77.78%,低于观察组的总有效率94.44%,差异有统计学意义(P<0.05)。与治疗前比较,观察组和对照组痛经症状积分均低于治疗前(P<0.05)。观察组治疗后改善程度大于对照组(P<0.01)。观察肾虚血瘀证候疗效总有效率为94.44%,高于对照组的55.56%,差异有统计学意义(P<0.05)。结论针刺配合中药灌肠治疗子宫腺肌症所致肾虚血瘀型痛经,效果显著,能更有效改善肾虚血瘀证证候、痛经程度。

基于网络药理学分析柴胡加龙骨牡蛎汤治疗不寐的作用机制

[目的]基于网络药理学技术探讨柴胡加龙骨牡蛎汤治疗不寐的作用机制。[方法]采用中药系统药理学分析平台(TCMSP),根据OB≥30%,DL≥0.18以及BATMAN数据库,查找柴胡加龙骨牡蛎汤方中的有效成分,通过Swiss Target Prediction平台进行靶点的预测分析,筛选潜在靶点;通过检索OMIM数据库、DiGSeE数据库及GeneCard数据库获取不寐的相关靶点,通过Cytoscape软件构建成分-靶点等多层次网络关联图。利用DAVID数据库对有效靶点进行GO和KEGG富集分析。[结果]从TCMSP、BATMAN中共筛选出203个活性成分,去除重复后得到161个活性成分,根据OB值和DL值选择草药排名前30的成分以及矿石类成分进行分析,共涉及302个潜在靶点。将从疾病数据库搜集到的2 620个不寐靶点通过Venny图的形式与潜在靶点取交集,共获得98个柴胡加龙骨牡蛎汤的有效作用靶点。通过DAVID数据库对有效靶点进行GO和KEGG富集分析,根据P值由小到大排列,选取P<0.05的富集结果进行分析。[结论]本研究分析了柴胡加龙骨牡蛎汤的多成分、多靶点、多途径及其相关成分-靶点网络效应图,为其治疗不寐的机制研究提供了数据网络支持,也为后期实验研究奠定了基础。

低剂量三苯基锡暴露对鲤GH/IGF-I轴的毒性效应

为探究环境内分泌物对鱼类生长的毒性影响及作用机制,以鲤(Cyprinus carpio)为实验动物,利用三苯基氯化锡(TPT)为目标毒物,设定对照组和0. 024、0. 24、2. 4μg/L三个TPT暴露组,不同时间(7、21、48 d)暴露取样,通过检测血浆生长激素(GH)和胰岛素样生长因子(IGF-I)水平及肝脏生长激素受体(GHR)、IGF-I基因和脑垂体GH基因的表达量,探讨三苯基锡暴露对鲤GH/IGF-I轴毒性效应的影响及其机制。结果表明:暴露7 d时,高浓度的TPT(0. 24、2. 4μg/L)促进垂体GH的合成与分泌,而IGF-I的合成与分泌及肝脏IGF-I基因的表达受到显著抑制;暴露21、48 d时,血浆GH、IGF-I含量显著下降,脑垂体GH基因及肝脏GHR、IGF-I基因的表达均受到显著性抑制且呈剂量效应关系。研究结果表明,鲤血浆GH、IGF-I含量及肝脏GHR、IGF-I基因和垂体GH基因的表达量对不同浓度的TPT反应敏感,可以作为监测环境污染物对鱼类GH/IGF-I轴毒性影响的指示因子。

熔融盐–亚临界汽/水传热性能实验分析

熔融盐是太阳能热电联产系统中一种新型的蓄热介质,实验以60%Na NO3和40%KNO3混合二元盐为工质,分别研究熔融盐与亚临界蒸汽和亚临界水在套管中传热特性。实验结果表明：熔融盐与亚临界汽/水换热时,熔熔融盐进口温度对熔融盐努塞尔特数影响较大,而蒸汽或水因压力改变影响自身的物性的变化对其影响很小。根据实验数据并采用多元线性拟合熔融盐努塞尔特数实验关联式,其最大拟合偏差分别是±10%、±5%。并与经典圆管对流传热DittusBoelter、Sieder-Tate、Михеев、Gnielinski关联式比较,其计算值均比实验值大,其最大偏差在-20%- -40%。

健肝颗粒联合恩替卡韦治疗乙肝肝硬化代偿期的临床疗效观察

目的:研究健肝颗粒联合恩替卡韦治疗乙肝肝硬化代偿期临床疗效。方法:60例乙肝肝硬化代偿期患者分为对照组和观察组,两组患者均口服恩替卡韦进行治疗,观察组增服健肝颗粒,为期3个月。采用全自动生化仪检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBil)和白蛋白(ALB),放射免疫分析法检测透明质酸(HA)、层连蛋白(LN)、Ⅲ型前胶原肽(PCⅢ)和Ⅳ型胶原(Ⅳ-C),使用Fi-broscan检测肝脏硬度值。结果:相较于治疗前,对照组和观察组治疗后的ALT、AST、TBil、HA、LN、PCⅢ和Ⅳ-C水平及肝脏硬度值均明显下降(P<0.05),ALB水平明显升高,差异有统计学意义(P<0.05);相较于对照组治疗后,观察组治疗后的ALT、AST、TBil、HA、LN、PCⅢ和Ⅳ-C水平及肝脏硬度值均明显下降,ALB水平明显升高(P<0.05)。结论:健肝颗粒联合恩替卡韦治疗乙肝肝硬化代偿期疗效显著,可有效改善肝功能,有抗肝纤维化作用。

绳状青霉P-19不同固体发酵浸提液对马铃薯镰刀菌根腐病病原菌的抑制作用

近年来,由镰刀菌引起的马铃薯根腐病严重影响我国马铃薯产业的发展。本研究通过比较不同发酵产物的抑菌活性,筛选绳状青霉Penicillium funiculosum P-19菌株最适的固体发酵培养基配方,并通过平板对峙试验测定了其对马铃薯根腐病病原菌尖孢镰刀菌Fusarium oxysporum、接骨木镰刀菌F.sambucinum、茄病镰刀菌F.solani、木贼镰刀菌F.equiseti和锐顶镰刀菌F.acuminatum的抑制作用。结果表明,绳状青霉P-19对尖孢镰刀菌、接骨木镰刀菌、茄病镰刀菌、木贼镰刀菌、锐顶镰刀菌的抑制率分别为38.85%、45.07%、38.92%、28.13%、64.32%。不同培养基发酵后其浸提液对5种镰刀菌的拮抗作用存在较大差异,其中配方为甘草∶玉米粉∶木屑=5∶4∶1时发酵产物对尖孢镰刀菌的抑制率最高,为67.89%,板蓝根∶玉米粉∶木屑=3∶5∶2时对接骨木镰刀菌的抑制率最高,为67.00%,板蓝根∶玉米粉∶木屑=3∶5∶2时对茄病镰刀菌的抑制率为64.48%,甘草∶板蓝根∶葡萄皮渣∶玉米粉=2∶2∶10∶5时对木贼镰刀菌的抑制率为50.20%,甘草∶玉米粉∶木屑=3∶5∶2时对锐顶镰刀菌的抑制率达到78.25%。本研究为马铃薯镰刀菌根腐病的防控以及青霉菌生防制剂的开发和应用奠定了基础。