Distinct molecular targets of ProEGCG from EGCG and superior inhibition of angiogenesis signaling pathways for treatment of endometriosis

Endometriosis is a common chronic gynecological disease with endometrial cell implantation outside the uterus.Angiogenesis is a major pathophysiology in endometriosis.Our previous studies have demonstrated that the prodrug of epigallocatechin gallate(ProEGCG)exhibits superior anti-endometriotic and anti-angiogenic effects compared to epigallocatechin gallate(EGCG).However,their direct binding targets and underlying mechanisms for the differential effects remain unknown.In this study,we demonstrated that oral ProEGCG can be effective in preventing and treating endometriosis.Additionally,1D and 2D Proteome Integral Solubility Alteration assay-based chemical proteomics identified metadherin(MTDH)and PX domain containing serine/threonine kinase-like(PXK)as novel binding targets of EGCG and ProEGCG,respectively.Computational simulation and BioLayer interferometry were used to confirm their binding affinity.Our results showed that MTDH-EGCG inhibited protein kinase B(Akt)-mediated angiogenesis,while PXK-ProEGCG inhibited epidermal growth factor(EGF)-mediated angiogenesis via the EGF/hypoxia-inducible factor(HIF-1a)/vascular endothelial growth factor(VEGF)pathway.In vitro and in vivo knockdown assays and microvascular network imaging further confirmed the involvement of these signaling pathways.Moreover,our study demonstrated that ProEGCG has superior therapeutic effects than EGCG by targeting distinct signal transduction pathways and may act as a novel antiangiogenic therapy for endometriosis.

Research on intelligent search-and-secure technology in accelerator hazardous areas based on machine vision

Prompt radiation emitted during accelerator operation poses a significant health risk,necessitating a thorough search and securing of hazardous areas prior to initiation.Currently,manual sweep methods are employed.However,the limitations of manual sweeps have become increasingly evident with the implementation of large-scale accelerators.By leveraging advancements in machine vision technology,the automatic identification of stranded personnel in controlled areas through camera imagery presents a viable solution for efficient search and security.Given the criticality of personal safety for stranded individuals,search and security processes must be sufficiently reliable.To ensure comprehensive coverage,180°camera groups were strategically positioned on both sides of the accelerator tunnel to eliminate blind spots within the monitoring range.The YOLOV8 network model was modified to enable the detection of small targets,such as hands and feet,as well as larger targets formed by individuals near the cameras.Furthermore,the system incorporates a pedestrian recognition model that detects human body parts,and an information fusion strategy is used to integrate the detected head,hands,and feet with the identified pedestrians as a cohesive unit.This strategy enhanced the capability of the model to identify pedestrians obstructed by equipment,resulting in a notable improvement in the recall rate.Specifically,recall rates of 0.915 and 0.82were obtained for Datasets 1 and 2,respectively.Although there was a slight decrease in accuracy,it aligned with the intended purpose of the search-and-secure software design.Experimental tests conducted within an accelerator tunnel demonstrated the effectiveness of this approach in achieving reliable recognition outcomes.

The BEL1-like transcription factor GhBLH5-A05 participates in cotton response to drought stress

Drought stress impairs crop growth and development.BEL1-like family transcription factors may be involved in plant response to drought stress,but little is known of the molecular mechanism by which these proteins regulate plant response and defense to drought stress.Here we show that the BEL1-like transcription factor GhBLH5-A05 functions in cotton(Gossypium hirsutum)response and defense to drought stress.Expression of GhBLH5-A05 in cotton was induced by drought stress.Overexpression of GhBLH5-A05 in both Arabidopsis and cotton increased drought tolerance,whereas silencing GhBLH5-A05 in cotton resulted in elevated sensitivity to drought stress.GhBLH5-A05 binds to cis elements in the promoters of GhRD20-A09 and GhDREB2C-D05 to activate the expression of these genes.GhBLH5-A05 interacted with the KNOX transcription factor GhKNAT6-A03.Co-expression of GhBLH5-A05 and GhKNAT6-A03 increased the transcription of GhRD20-A09 and GhDREB2C-D05.We conclude that GhBLH5-A05 acts as a regulatory factor with GhKNAT6-A03 functioning in cotton response to drought stress by activating the expression of the drought-responsive genes GhRD20-A09 and GhDREB2C-D05.

仑伐替尼通过调节肿瘤免疫微环境协同增强免疫检查点抑制剂对肝细胞癌的疗效

目的:探讨仑伐替尼(lenvatinib, Len)增强免疫检查点抑制剂在小鼠肝细胞癌(hepatocellular carcinoma, HCC)中的疗效,并分析其在肿瘤微环境中的免疫调节机制。方法:分析不同浓度的Len对人脐静脉血管内皮细胞迁移和CXC趋化因子配体10(CXC chemokine ligand 10, CXCL10)分泌的影响并分析Len影响CXCL10分泌的机制。构建小鼠原位HCC模型,将荷瘤小鼠随机分为PBS组、BMS-202(PD-1/PD-L1抑制剂)组、Len组和Len/BMS-202组,通过小动物活体成像观察小鼠原位HCC的生长情况。治疗第13天处死小鼠取肿瘤组织,免疫荧光法检测肿瘤组织凋亡、血管结构和缺氧情况。免疫组化法检测肿瘤组织内增殖标志物Ki67和转化生长因子β(transforming growth factor-β, TGF-β)的表达水平,及CD4+T细胞、CD8+T细胞的浸润程度。ELISA检测小鼠血清中的免疫因子干扰素γ(interferon-γ, IFN-γ)、CXCL10和TGF-α的分泌情况,进行统计学分析。结果:(1)Len在低剂量范围内可促进内皮细胞迁移,且Len通过阻断FGFR增强肿瘤细胞对IFN-γ的响应,进而促进肿瘤细胞分泌CXCL10。(2)与PBS组相比,各给药组肿瘤生长均较缓慢,其中以Len/BMS-202组荷瘤小鼠肿瘤生长受抑制为著(P<0.05)。(3)与PBS组及单药组相比,Len/BMS-202组明显促进了肿瘤组织的凋亡和抑制肿瘤细胞的增殖(P<0.05)。(4)与PBS组及BMS-202组相比,Len组及Len/BMS-202组肿瘤组织周细胞覆盖率明显提升(P<0.01),缺氧状态明显缓解(P<0.01)。(5)与PBS组及单药组相比,Len/BMS-202组肿瘤内CD4+T细胞和CD8+T细胞浸润明显增加(P<0.01),TGF-β的表达显著下降(P<0.01)。(6)与PBS组相比,各治疗组不同程度促进了小鼠血清中IFN-γ、CXCL10和TGF-α的分泌(P<0.05),其中Len/BMS-202组效果最好(P<0.01)。结论:Len可能通过促进肿瘤血管正常化、改善缺氧及促进CXCL10分泌,共同激活肿瘤免疫微环境,从而协同增强BMS-202对HCC的治疗效果。

Age-specific differences in the association between prediabetes and cardiovascular diseases in China:A national cross-sectional study

BACKGROUND Cardiovascular disease(CVD)is a leading cause of morbidity and mortality worldwide,the global burden of which is rising.It is still unclear the extent to which prediabetes contributes to the risk of CVD in various age brackets among adults.To develop a focused screening plan and treatment for Chinese adults with prediabetes,it is crucial to identify variations in the connection between prediabetes and the risk of CVD based on age.AIM To examine the clinical features of prediabetes and identify risk factors for CVD in different age groups in China.METHODS The cross-sectional study involved a total of 46239 participants from June 2007 through May 2008.A thorough evaluation was conducted.Individuals with prediabetes were categorized into two groups based on age.Chinese atherosclerotic CVD risk prediction model was employed to evaluate the risk of developing CVD over 10 years.Random forest was established in both age groups.SHapley Additive exPlanation method prioritized the importance of features from the perspective of assessment contribution.RESULTS In total,6948 people were diagnosed with prediabetes in this study.In prediabetes,prevalences of CVD were 5(0.29%)in the younger group and 148(2.85%)in the older group.Overall,11.11%of the younger group and 29.59% of the older group were intermediate/high-risk of CVD for prediabetes without CVD based on the Prediction for ASCVD Risk in China equation in ten years.In the younger age group,the 10-year risk of CVD was found to be more closely linked to family history of CVD rather than lifestyle,whereas in the older age group,resident status was more closely linked.CONCLUSION The susceptibility to CVD is age-specific in newly diagnosed prediabetes.It is necessary to develop targeted approaches for the prevention and management of CVD in adults across various age brackets.

Evaluation of G3BP1 in the prognosis of acute and acute-on-chronic liver failure after the treatment of artificial liver support system

BACKGROUND The increased expression of G3BP1 was positively correlated with the prognosis of liver failure.AIM To investigate the effect of G3BP1 on the prognosis of acute liver failure(ALF)and acute-on-chronic liver failure(ACLF)after the treatment of artificial liver support system(ALSS).METHODS A total of 244 patients with ALF and ACLF were enrolled in this study.The levels of G3BP1 on admission and at discharge were detected.The validation set of 514 patients was collected to verify the predicted effect of G3BP1 and the viability of prognosis.RESULTS This study was shown that lactate dehydrogenase(LDH),alpha-fetoprotein(AFP)and prothrombin time were closely related to the prognosis of patients.After the ALSS treatment,the patient’amount of decreased G3BP1 index in difference of G3BP1 between the value of discharge and admission(difG3BP1)<0 group had a nearly 10-fold increased risk of progression compared with the amount of increased G3BP1 index.The subgroup analysis showed that the difG3BP1<0 group had a higher risk of progression,regardless of model for end-stage liver disease high-risk or low-risk group.At the same time,compared with the inflam matory marks[tumor necrosis factor-α,interleukin(IL)-1βand IL-18],G3BP1 had higher discrimination and was more stable in the model analysis and validation set.When combined with AFP and LDH,concordance index was respectively 0.84 and 0.8 in training and validation cohorts.CONCLUSION This study indicated that G3BP1 could predict the prognosis of ALF or ACLF patients treated with ALSS.The combination of G3BP1,AFP and LDH could accurately evaluate the disease condition and predict the clinical endpoint of patients.

Exploring Effective Ways of Green Printing to Reduce Harmful Volatile Organic Compounds Emissions

This paper delves into the transformative shift in the printing industry from traditional petroleum-based inks to sustainable alternatives,focusing on soy ink.Initially,it examines the environmental and health hazards associated with conventional printing,highlighting the detrimental impact of volatile organic compounds(VOCs)and toxic substances in inks.The emergence of soy ink as an eco-friendly solution is then explored.Derived from soybeans,soy ink significantly reduces the release of harmful VOCs and enhances the recyclability of printed materials.The paper discusses not only the environmental benefits of soy ink but also its operational and economic advantages,such as improved deinking capabilities and waste reduction.A notable development in soy ink technology is the use of soy methyl ester,which addresses the challenges of slow drying and penetration associated with traditional inks.The paper concludes by emphasizing the need for continued innovation in sustainable practices within the printing industry,positioning soy ink as a key player in aligning economic goals with environmental responsibility.The shift to soy-based inks exemplifies a broader trend towards sustainability,pivotal for the future health of the planet.

并购重组业绩承诺与内部人减持

业绩承诺作为我国企业并购活动的重要内容之一,既可能是激发企业价值创造的利器,也可能沦为内部人自利的工具。本文以2008-2019年的A股上市公司为样本,研究并购重组业绩承诺对内部人减持行为的影响。研究发现,内部人在业绩承诺期内减持规模显著增加,尤其当公司盈余信息质量较低、分析师乐观度较高时,内部人减持规模更大。当公司业绩承诺实现率虚高时,内部人减持也更多。这些结果表明,内部人可能会利用自身信息优势在承诺期内高位减持套现,导致中小投资者利益受损。此外,本文还发现业绩承诺对内部人减持的影响在非关联并购、民营企业及外部媒体监督较弱的企业中更显著。进一步研究表明,内部人主要在业绩承诺期后期减持,并且当业绩承诺水平越高时,内部人减持规模越大。本文的研究有助于拓展业绩承诺对内部人减持的影响机理研究,也有助于丰富研究内部人信息优势和自利行为的相关文献,同时对于完善业绩承诺监管和改善投资者决策均具有重要的参考意义。

Adaptive Stochastic Synchronization of Uncertain Delayed Neural Networks

This paper considers adaptive synchronization of uncertain neural networks with time delays and stochastic perturbation. A general adaptive controller is designed to deal with the difficulties deduced by uncertain parameters and stochastic perturbations, in which the controller is less conservative and optimal since its control gains can be automatically adjusted according to some designed update laws. Based on Lyapunov stability theory and Barbalat lemma, sufficient condition is obtained for synchronization of delayed neural networks by strict mathematical proof. Moreover, the obtained results of this paper are more general than most existing results of certainly neural networks with or without stochastic disturbances. Finally, numerical simulations are presented to substantiate our theoretical results.

发酵豆渣和蛋白桑对蛋鸡生长性能、生产性能和蛋品质的影响

本试验旨在研究发酵豆渣(FSR)以及蛋白桑与豆渣混合发酵产物(FMASR)对蛋鸡生长性能、生产性能和蛋品质的影响。生长阶段:选取体重相近的47日龄华北柴鸡300只进行林下养殖,随机分成3组,每组4个重复,每个重复25只鸡,对照组(C组)饲喂基础日粮,试验1组(F1组)添加10%FSR,试验2组(F2组)添加10%FMASR,试验期21 d。试验结束后继续饲喂相应日粮至120日龄。产蛋阶段:从各试验组的每个重复选取15只鸡作为产蛋阶段的重复,对应为产蛋阶段的C组、F1组、F2组,分别饲喂基础日粮、含10%FSR的日粮、含10%FMASR的日粮,试验期98 d。结果表明:(1)与对照组相比,日粮中添加10%FSR和10%FMASR对蛋鸡平均日采食量无显著影响(P>0.05),但蛋鸡的日增重分别显著提高3.83%(P<0.05)和8.40%(P<0.01),料重比分别显著降低3.20%(P<0.05)和6.40%(P<0.01);(2)与对照组相比,日粮中添加10%FMASR,可以促进蛋鸡提前8 d开产和提前2周进入产蛋高峰期,产蛋高峰期的产蛋率、日产蛋重、平均蛋重、蛋白高度、哈氏单位、蛋黄a*值、蛋黄b*值分别显著提高6.55%(P<0.01)、4.22%(P<0.05)、3.97%(P<0.05)、26.2%(P<0.05)、11.63%(P<0.01)、10.40%(P<0.05)、7.44%(P<0.05),料蛋比显著降低3.77%(P<0.05),对蛋形指数、蛋壳强度、蛋壳厚度和蛋黄L*值无显著影响(P>0.05);(3)与对照组相比,日粮中添加10%FSR可以促进蛋鸡提前4 d开产和提前1周进入产蛋稳定期,但对产蛋高峰期的产蛋率日产蛋重、平均蛋重、料蛋比和蛋品质的各项指标无显著影响(P>0.05)。由此可见,日粮中添加10%FMASR可显著提高蛋鸡的生长性能、产蛋初期及产蛋高峰期生产性能和产蛋高峰期的蛋品质,日粮中添加10%FSR可显著提高蛋鸡生长性能和产蛋初期的生产性能。

从免疫微环境角度探讨健脾化湿法在大肠癌中的运用

大肠癌是临床中常见的恶性肿瘤,随着精准医学的不断发展,肿瘤免疫疗法日益成为重要的治疗手段。免疫微环境与大肠癌的发生发展、预后和免疫治疗反应密切相关,免疫微环境作为肿瘤微环境中的主要组成部分,其作用值得关注。中医药治疗作为肿瘤综合治疗的一部分,在个体化防治大肠癌中具有重要的地位,且安全性较高。运用扶正祛邪之法治疗肿瘤能够提高机体免疫功能,进而抑制肿瘤进展,改善生活质量等。健脾化湿法是中医治疗大肠癌常用的治法,以免疫微环境为切入点,从中医理论深入探讨健脾化湿法在调节大肠癌免疫微环境中的作用机制具有重要意义,并将为中医在调节免疫微环境并改善大肠癌免疫治疗的疗效提供相应的理论依据。

预测性编码在视听觉神经活动中的典型表征

现代神经科学研究指出,大脑是外部世界的“预测器”,它能根据先验知识和当前信息对即将到来的感觉信息进行主动估计,从而完成与外部世界的高效交互。预测性编码是描述预期作用机制的主要理论模型,梳理其在解释视、听觉神经现象方面的研究进展,可为深入理解大脑工作模式提供新的理论基础。本文简述了预测性编码的内容;从常用范式、典型现象、面临争议等方面梳理预期与感觉输入相互作用的典型研究;从有预期无刺激的神经表征、预期相关神经振荡模式两方面简述预期独立于刺激的内源性神经表征;进而回顾了支持预测性编码中分级结构的神经生理证据及重要神经结构。最后,本文从深化理论研究、助力疾病诊疗、启发脑-机接口技术等方面对预测性编码相关研究的发展进行了展望。深入理解预测性编码在视、听觉神经活动中的计算模型及神经表征,有望为揭示大脑感知觉神经活动工作模式开辟新途径。

基于改进YOLOv5的矿区输电线路鸟群检测算法

针对矿区输电线路图像背景复杂、鸟类目标较小、目标聚集检测困难的问题,提出一种改进的YOLOv5目标检测算法进行矿区输电线路鸟群检测。首先,针对矿区输电线路图像中不同尺度的目标,使用Kmeans++算法来聚类锚框,以生成更符合数据集目标的一组锚框,从而提高目标检测精度和加速模型拟合;其次,通过在模型中新增一个检测层来充分利用网络模型的浅层语义信息,实现对微小目标的精准检测;最后,通过施加CBAM注意力,进一步增强对特征信息的利用。为验证所提算法的有效性,在矿区输电线路数据集上进行实验,结果表明所提算法较原算法平均精度提升5.2%,对小目标和密集鸟类目标具有更好的检测效果。

Neuroprotective effect of mesenchymal stem cellderived extracellular vesicles on optic nerve injury in chronic ocular hypertension

Mesenchymal stem cells have neuroprotective effects that limit damage to the retina and photoreceptors,and which may be mediated by extracellular vesicles(or exosomes)released by mesenchymal stem cells.To investigate the neuroprotective effect of extracellular vesicles derived from umbilical cord mesenchymal stem cells on glaucoma,we established rat models of chronic ocular hypertension by injecting conjunctival fibroblasts into the anterior chamber to mimic optic nerve injury caused by glaucoma.One week after injury,extracellular vesicles derived from umbilical cord-derived mesenchymal stem cells were injected into the vitreous cavity.We found that extracellular vesicles derived from mesenchymal stem cells substantially reduced retinal damage,increased the number of retinal ganglion cells,and inhibited the activation of caspase-3.These findings suggest that mesenchymal stem cell-derived extracellular vesicles can help alleviate optic nerve injury caused by chronic ocular hypertension,and this effect is achieved by inhibiting cell apoptosis.

基于WPT和SSA-BP的直流充电桩充电模块故障诊断

充电模块是电动汽车直流充电桩最为关键的部分,针对其功率开关和电解电容等核心器件的开路故障特点,提出了一种基于小波包变换(wavelet packet transform,WPT)和麻雀搜索算法优化反向传播神经网络(sparrow search algorithm-back propagation,SSA-BP)的故障诊断方法。该方法以充电模块输出电压为原始信号,首先通过预处理剔除其直流分量,对处理后的信号进行小波包分解,然后计算出各子频带信号的能量,通过归一化得到初始特征向量,最后将直流分量和归一化后的特征向量作为最终故障特征量输入到SSA-BP神经网络,进而输出分类结果实现故障诊断。为验证方法的可行性和优越性,搭建了输出功率15 kW的两级仿真模型,在不同工况下进行仿真验证。实验结果表明该方法具有较好的诊断效果,诊断率达到93.85%,对电动汽车直流充电桩故障诊断具有现实指导意义。

Stress granules inhibit endoplasmic reticulum stress-mediated apoptosis during hypoxia-induced injury in acute liver failure

BACKGROUND Stress granules(SGs)could be formed under different stimulation to inhibit cell injury.AIM To investigate whether SGs could protect hepatocytes from hypoxia-induced damage during acute liver failure(ALF)by reducing endoplasmic reticulum stress(ERS)mediated apoptosis.METHODS The agonist of SGs,arsenite(Ars)was used to intervene hypoxia-induced hepatocyte injury cellular model and ALF mice models.Further,the siRNA of activating transcription factor 4(ATF4)and SGs inhibitor anisomycin was then used to intervene in cell models.RESULTS With the increase of hypoxia time from 4 h to 12 h,the levels of HIF-1α,ERS and apoptosis gradually increased,and the expression of SGs marker G3BP1 and TIA-1 was increased and then decreased.Compared with the hypoxia cell model group and ALF mice model,the levels of HIF-1α,apoptosis and ERS were increased in the Ars intervention group.After siRNA-ATF4 intervention,the level of SGs in cells increased,and the levels of HIF-1α,ERS and apoptosis decreased.Compared with the siRNA-ATF4 group,the levels of G3BP1 in the siRNAATF4+anisomycin group were decreased,and the levels of HIF-1α,ERS and apoptosis were increased.Moreover,compared with the ALF group,the degree of liver injury and liver function,the levels of HIF-1α,ERS and apoptosis in the Ars intervention group were decreased,the level of SGs was increased.CONCLUSION SGs could protect hepatocytes from hypoxia-induced damage during ALF by reducing ERSmediated apoptosis.

Solid polymer electrolytes in all-solid-state lithium metal batteries:From microstructures to properties

All-solid-state lithium(Li)metal batteries(ASSLMBs)are considered one of the most promising secondary batteries due to their high theoretical capacity and high safety performance.However,low room-temperature ionic conductivity and poor interfacial stability are two key factors affecting the practical application of ASSLMBs,and our understanding of the mechanisms behind these key problems from microscopic perspective is still limited.In this review,the mechanisms and advanced characterization techniques of ASSLMBs are summarized to correlate the microstructures and properties.Firstly,we summarize the challenges faced by solid polymer electrolytes(SPEs)in ASSLMBs,such as the low roomtemperature ionic conductivity and the poor interfacial stability.Secondly,several typical improvement methods of polymer ASSLMBs are discussed,including composite SPEs,ultra-thin SPEs,SPEs surface modification and Li anode surface modification.Finally,we conclude the characterizations for correlating the microstructures and the properties of SPEs,with emphasis on the use of emerging advanced techniques(e.g.,cryo-transmission electron microscopy)for in-depth analyzing ASSLMBs.The influence of the microstructures on the properties is very important.Until now,it has been difficult for us to understand the microstructures of batteries.However,some recent studies have demonstrated that we have a better understanding of the microstructures of batteries.Then we suggest that in situ characterization,nondestructive characterization and sub-angstrom resolution are the key technologies to help us further understand the batteries'microstructures and promote the development of batteries.And potential investigations to understand the microstructures evolution and the batteries behaviors are also prospected to expect further reasonable theoretical guidance for the design of ASSLMBs with ideal performance.

Resequencing of sweetpotato germplasm resources reveals key loci associated with multiple agronomic traits

Sweetpotato is an important crop that exhibits hexaploidy and high heterozygosity,which limits gene mining for important agronomic traits.Here,314 sweetpotato germplasm resources were deeply resequenced,and 4599509 SNPs and 846654 InDels were generated,among which 196124 SNPs were nonsynonymous and 9690 InDels were frameshifted.Based on the Indels,genome-wide marker primers were designed,and 3219 of 40366 primer pairs were selected to construct the core InDel marker set.The molecular ID of 104 sweetpotato samples verified the availability of these primers.The sweetpotato population structures were then assessed through multiple approaches using SNPs,and diverse approaches demonstrated that population stratification was not obvious for most Chinese germplasm resources.As many as 20 important agronomic traits were evaluated,and a genome-wide association study was conducted on these traits.A total of 19 high-confidence loci were detected in both models.These loci included several candidate genes,such as IbMYB1,IbZEP1,and IbYABBY1,which might be involved in anthocyanin metabolism,carotenoid metabolism,and leaf morphogenesis,respectively.Among them,IbZEP1 and IbYABBY1 were first reported in sweetpotato.The variants in the promoter and the expression levels of IbZEP1 were significantly correlated with f lesh color(orange or not orange)in sweetpotato.The expression levels of IbYABBY1 were also correlated with leaf shape.These results will assist in genetic and breeding studies in sweetpotato.

低扬程泵站更新改造关键技术

合理地选择设计流量和如何使水泵在特征扬程变化的情况下保持较高的效率是泵站改造的关键。如果设计流量偏大,水泵内部的流速变大,空化性能下降,可能产生空蚀振动。对于扬程变幅较大的泵站,应当采用定制式设计方法为泵站专门开发高效区宽广的水力模型,提高泵站的平均效率。

小鼠衰老所致“正虚”过程中免疫功能衰退表征的特点

目的:基于小鼠渐进衰老模型探讨衰老所致“正虚”的免疫功能衰退表征的特点。方法:使用不同月龄(2、6、15月龄)C57BL/6小鼠,通过流式细胞术检测并比较小鼠外周血和脾组织中T细胞、髓源性抑制细胞(MDSC)及其亚群的丰度变化。结果:外周血中T细胞亚群表型为CD3^(+)CD4^(+)CD44-CD62L^(+)的幼稚CD4^(+)T细胞(2 vs 6月龄,P=0.137;2 vs 15月龄,P=0.004;6 vs15月龄,P=0.105)和表型为CD3^(+)CD8^(+)CD44-CD62L^(+)的幼稚CD8^(+)T细胞(2 vs 6月龄,P=0.179;2 vs 15月龄,P=0.001;6 vs15月龄,P=0.015)出现与衰老有关的细胞比例降低,差异具有统计学意义。表型为CD3^(+)CD4^(+)CD44^(+)CD62L^(+)的中央记忆CD8^(+)T细胞出现与衰老有关的比例升高,差异具有统计学意义(2 vs 6月龄,P=0.01;2 vs 15月龄,P=0.007;6 vs 15月龄,P=0.164)。对脾组织的检测结果具有与外周血相同特点。同时,CD8^(+)T细胞比例随衰老逐渐升高(2 vs 6月龄,P=0.027;2 vs 15月龄,P<0.001;6 vs15月龄,P<0.001);表型为CD8^(+)CD28^(+)的活化CD8^(+)T细胞亚群比例也出现随月龄增长的上升(2 vs 6月龄,P=0.863;2 vs 15月龄,P=0.016;6 vs 15月龄,P=0.024),差异均具有统计学意义。结论:衰老所致“正虚”过程中,不同免疫细胞亚群变化并不都反映免疫抑制特点,虽然总体免疫功能下降,但单一表型难以反应整体免疫功能变化。