LBD(Iateral organ boundaries domain)/ASL(Asymmetric leaves2-like)是一类具有LOB(Iateral organ boundaries)结构域的转录因子,主要参与植物生物与非生物应答。其中LBD37能够通过抑制PAP1和PAP2参与调控花青素积累。花青素作为一种...LBD(Iateral organ boundaries domain)/ASL(Asymmetric leaves2-like)是一类具有LOB(Iateral organ boundaries)结构域的转录因子,主要参与植物生物与非生物应答。其中LBD37能够通过抑制PAP1和PAP2参与调控花青素积累。花青素作为一种重要的类黄酮化合物广泛存在于植物体内,花青素的积累能有效地增强植物抗性。MeLBD47作为LBD37同源物,在木薯中发挥的功能尚不清晰。为了更好地研究MeLBD47,采用生物信息学手段分析了MeLBD47的基本理化性质、亲水性、蛋白质二级结构以及磷酸化位点,利用VIGS手段,验证MeLBD47对花青素的积累的影响。结果表明Me LBD47分子质量为27106.93,等电点为8.61,为亲水性蛋白,不包跨膜结构。MeLBD47蛋白主要有由无规则卷曲、α-螺旋结构和β-转角结构以及延伸链构成。RT-PCR结果显示Me LBD47全长为744 bp,编码247个氨基酸。RT-qPCR结果显示MeLBD47主要在根中表达。VIGS沉默MeLBD47,木薯花青素含量显著增加23.49%。综上所述,MeLBD47在花青素合成途径发挥重要作用。本研究为丰富花青素调控机制及木薯遗传育种提供一定理论依据。展开更多
Objective To observe the effect of electroacupuncture(EA)of“concurrent treatment of the brain and heart”on angiogenesis and cortical vascular endothelial growth factor(VEGF)and brain-derived neurotrophic factor(BDNF...Objective To observe the effect of electroacupuncture(EA)of“concurrent treatment of the brain and heart”on angiogenesis and cortical vascular endothelial growth factor(VEGF)and brain-derived neurotrophic factor(BDNF)in rats with focal cerebral ischemia,and to explore the mechanism of EA in cerebral ischemia treatment.Methods A total of 108 Sprague-Dawley rats,27 rats were randomly selected as the sham-operation group,and the rest rats received the right middle cerebral artery occlusion operation for model preparation firstly,and then were divided into a model group,a traditional acupoint group,and a concurrent treatment of the brain and heart group,with 27 rats in each group.In the sham-operation group,only the carotid artery was isolated.EA at Shuigou(CV26),Quchi(LI11),Hegu(LI4),and Zusanli(ST36)in the traditional acupoint group,and EA at Fengfu(GV16),Baihui(GV20),Xinshu(BL15),and Neiguan(PC6)in the concurrent treatment of the brain and heart group were performed 4 h after the operation,once a day,for 14 consecutive days.Rats in the sham-operation group and the model group were identically fixed without any treatment.Before and after treatment,the modified neurological severity score(mNSS),regional cerebral blood flow(rCBF),and CD34 positive expression by immunohistochemistry were measured.The positive protein expression levels of VEGF and BDNF were detected by immunofluorescence,and the mRNA expression levels of VEGF and BDNF were detected by quantitative reverse transcription-polymerase chain reaction(qRT-PCR).Results Compared with the sham-operation group,the mNSS,rCBF,and ischemic side cortical micro-vessel density(MVD)decreased,and the protein and mRNA expression levels of VEGF and BDNF increased in the model group(P<0.01).Compared with the model group,the mNSS of the two EA groups decreased,and the mNSS of the concurrent treatment of the brain and heart group was lower than that of the traditional acupoint group on the 14th day(P<0.05).Compared with the model group,the rCBF in the two EA groups increased,and the rCBF reached the highest on the 14th day(P<0.05 or P<0.01),and the rCBF in the concurrent treatment of the brain and heart group was higher than that in the traditional acupoint group(P<0.05);the MVD of the two EA groups was higher than that of the model group,and the MVD of the concurrent treatment of the brain and heart group was higher than that of the traditional acupoint group on the 7th and 14th days(P<0.05 or P<0.01).Compared with the model group,the protein and mRNA expression levels of VEGF and BDNF in the two EA groups increased(P<0.01).The VEGF expression level was the highest on the 7th day in the concurrent treatment of the brain and heart group(P<0.05),and the BDNF expression level was higher on the 7th and 14th days than on the 3rd day(P<0.05).The mRNA expression levels of VEGF and BDNF in both EA groups reached the highest on the 7th day(P<0.05 or P<0.01).Conclusion EA therapy can up-regulate the VEGF and BDNF expression levels and increase the rCBF in the cortex of rats with focal cerebral ischemia,which may be one mechanism of EA in the cerebral ischemia treatment.The therapeutic effect is accumulated with the effective time,and the concurrent treatment of the brain and heart group is superior to the traditional acupoint group in promoting angiogenesis.展开更多
文摘LBD(Iateral organ boundaries domain)/ASL(Asymmetric leaves2-like)是一类具有LOB(Iateral organ boundaries)结构域的转录因子,主要参与植物生物与非生物应答。其中LBD37能够通过抑制PAP1和PAP2参与调控花青素积累。花青素作为一种重要的类黄酮化合物广泛存在于植物体内,花青素的积累能有效地增强植物抗性。MeLBD47作为LBD37同源物,在木薯中发挥的功能尚不清晰。为了更好地研究MeLBD47,采用生物信息学手段分析了MeLBD47的基本理化性质、亲水性、蛋白质二级结构以及磷酸化位点,利用VIGS手段,验证MeLBD47对花青素的积累的影响。结果表明Me LBD47分子质量为27106.93,等电点为8.61,为亲水性蛋白,不包跨膜结构。MeLBD47蛋白主要有由无规则卷曲、α-螺旋结构和β-转角结构以及延伸链构成。RT-PCR结果显示Me LBD47全长为744 bp,编码247个氨基酸。RT-qPCR结果显示MeLBD47主要在根中表达。VIGS沉默MeLBD47,木薯花青素含量显著增加23.49%。综上所述,MeLBD47在花青素合成途径发挥重要作用。本研究为丰富花青素调控机制及木薯遗传育种提供一定理论依据。
文摘Objective To observe the effect of electroacupuncture(EA)of“concurrent treatment of the brain and heart”on angiogenesis and cortical vascular endothelial growth factor(VEGF)and brain-derived neurotrophic factor(BDNF)in rats with focal cerebral ischemia,and to explore the mechanism of EA in cerebral ischemia treatment.Methods A total of 108 Sprague-Dawley rats,27 rats were randomly selected as the sham-operation group,and the rest rats received the right middle cerebral artery occlusion operation for model preparation firstly,and then were divided into a model group,a traditional acupoint group,and a concurrent treatment of the brain and heart group,with 27 rats in each group.In the sham-operation group,only the carotid artery was isolated.EA at Shuigou(CV26),Quchi(LI11),Hegu(LI4),and Zusanli(ST36)in the traditional acupoint group,and EA at Fengfu(GV16),Baihui(GV20),Xinshu(BL15),and Neiguan(PC6)in the concurrent treatment of the brain and heart group were performed 4 h after the operation,once a day,for 14 consecutive days.Rats in the sham-operation group and the model group were identically fixed without any treatment.Before and after treatment,the modified neurological severity score(mNSS),regional cerebral blood flow(rCBF),and CD34 positive expression by immunohistochemistry were measured.The positive protein expression levels of VEGF and BDNF were detected by immunofluorescence,and the mRNA expression levels of VEGF and BDNF were detected by quantitative reverse transcription-polymerase chain reaction(qRT-PCR).Results Compared with the sham-operation group,the mNSS,rCBF,and ischemic side cortical micro-vessel density(MVD)decreased,and the protein and mRNA expression levels of VEGF and BDNF increased in the model group(P<0.01).Compared with the model group,the mNSS of the two EA groups decreased,and the mNSS of the concurrent treatment of the brain and heart group was lower than that of the traditional acupoint group on the 14th day(P<0.05).Compared with the model group,the rCBF in the two EA groups increased,and the rCBF reached the highest on the 14th day(P<0.05 or P<0.01),and the rCBF in the concurrent treatment of the brain and heart group was higher than that in the traditional acupoint group(P<0.05);the MVD of the two EA groups was higher than that of the model group,and the MVD of the concurrent treatment of the brain and heart group was higher than that of the traditional acupoint group on the 7th and 14th days(P<0.05 or P<0.01).Compared with the model group,the protein and mRNA expression levels of VEGF and BDNF in the two EA groups increased(P<0.01).The VEGF expression level was the highest on the 7th day in the concurrent treatment of the brain and heart group(P<0.05),and the BDNF expression level was higher on the 7th and 14th days than on the 3rd day(P<0.05).The mRNA expression levels of VEGF and BDNF in both EA groups reached the highest on the 7th day(P<0.05 or P<0.01).Conclusion EA therapy can up-regulate the VEGF and BDNF expression levels and increase the rCBF in the cortex of rats with focal cerebral ischemia,which may be one mechanism of EA in the cerebral ischemia treatment.The therapeutic effect is accumulated with the effective time,and the concurrent treatment of the brain and heart group is superior to the traditional acupoint group in promoting angiogenesis.
