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Research on the Space Construction of the Novel Ready Player One
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作者 yao yu-xin 《Journal of Literature and Art Studies》 2023年第3期165-169,共5页
The novel Ready Player One shows Ernest Cline’s ability of space construction,and demonstrates charm and artistic value of the virtual fantasy space it depicts.It is a science fiction with perfect combination of ideo... The novel Ready Player One shows Ernest Cline’s ability of space construction,and demonstrates charm and artistic value of the virtual fantasy space it depicts.It is a science fiction with perfect combination of ideological content and artistic form.This thesis aims to discuss the Ernest Cline’s construction of the virtual pace and the real space in Ready Player One and the function of their construction.In real space,the environment is awful and dilapidated,and people’s lives are depressed.While in the virtual space,the world is colorful and people have varied ways of entertainment to fulfill their mind.This Thesis summarizes the effects brought by both the real space and virtual space for Ready Player One.With such a strong contrast,the conflict between the two spaces promotes the narrative process and also highlights the theme of the novel. 展开更多
关键词 Ernest Cline Ready Player One space construction science fiction
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Polyphenolic Compound and the Degree of Browning in Processing Apple Varieties 被引量:17
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作者 SONG Ye yao yu-xin +3 位作者 ZHAI Heng DU Yuan-peng CHEN Feng WEI Shu-wei 《Agricultural Sciences in China》 CAS CSCD 2007年第5期607-612,共6页
Polyphenolic compound in processing apple (Malus domestica Borkh.) varieties and the relationship between polyphenol content and enzymatic browning were studied to provide reference for raw material selection and pr... Polyphenolic compound in processing apple (Malus domestica Borkh.) varieties and the relationship between polyphenol content and enzymatic browning were studied to provide reference for raw material selection and processing method optimization. The content of polyphenol compound in 10 processing apple varieties (4 cider and 6 juice varieties) were analyzed using the Folin-Ciocalteu method and HPLC. The degree of browning and the activities of polyphenol oxidase were also studied. The content and proportion of the polyphenol varied depending on the variety. Bitter varieties globally showed a higher polyphenol concentration than sweet or acid varieties. Proanthocyanidins, chlorogenic acid, (+)-catechin, (-)-epicatechin were high-concentrated polyphenols in apple fruits. Phloridzin, the unique polyphenol of apple, was abundant in the bitter variety Frequin rouge fruit. Total polyphenols, proanthocyanidins, (+)-catechin, and phloridzin had higher correlations with browning. The correlation was low between chlorogenic acid and browning. The polyphenolic profiles were correlated with the apple types. Cider apples contained more polyphenol than juice apple varieties. The content of flavan-3-ol has a close relationship with fruit browning. 展开更多
关键词 APPLE processing varieties polyphenolic compounds BROWNING polyphenol oxidase
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基于跨反向剪接位点引物特异性检测circRNA的PCR方法
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作者 孙宝箴 全龙萍 +5 位作者 康慧 姚玉新 沈甜 陈卫平 杜远鹏 高振 《生物技术通报》 CAS CSCD 北大核心 2022年第5期279-285,共7页
为了特异扩增circRNA发生可变反向剪接环化事件时被包含的circRNA,准确分析目标circRNA的表达水平。首先通过生物信息学分析葡萄高可信度circRNA的可变反向剪接环化事件特征,提出一种适用于葡萄circRNA可变反向剪接环化事件的引物设计... 为了特异扩增circRNA发生可变反向剪接环化事件时被包含的circRNA,准确分析目标circRNA的表达水平。首先通过生物信息学分析葡萄高可信度circRNA的可变反向剪接环化事件特征,提出一种适用于葡萄circRNA可变反向剪接环化事件的引物设计改良方法,即一端引物的3'端跨反向剪接位点3-4个碱基,并运用RT-PCR和qRT-PCR方法进行验证。结果表明,高可信度葡萄circRNA的来源基因中有21.7%存在可变反向剪接环化事件,可分为并列、交叉和包含关系。选取4组circRNA进行扩增,其中circRNA_4363、circRNA_6017、circRNA_6044和circRNA_7086分别被circRNA_4364、circRNA_6018、circRNA_6045和circRNA_7085包含,使用常规背向引物对被包含的4个circRNA进行RT-PCR可获得2条扩增条带,且qRT-PCR溶解曲线为双峰。通过应用改良引物对circRNA_4363、circRNA_6017和circRNA_7086进行RT-PCR和qRT-PCR可获得单一扩增产物。相较于常规背向引物,使用改良引物可以特异扩增发生可变反向剪接环化事件时被包含的circRNA,使其定量分析结果更加准确可靠。 展开更多
关键词 circRNA QRT-PCR 背向引物 可变反向剪接
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首件鉴定在柴油机新机型零部件开发中的应用探究
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作者 姚玉欣 黄瑛 《内燃机与配件》 2022年第17期14-16,共3页
首件鉴定作为实施产品质量控制的重要手段之一,新机型开发中除标准件、借用件之外的零部件,均可通过其来验证产品可制造性、工艺可操作性及合理性、设备精度及生产能力等,以保证零部件质量。本文针对柴油机新机型零部件开发中首件鉴定... 首件鉴定作为实施产品质量控制的重要手段之一,新机型开发中除标准件、借用件之外的零部件,均可通过其来验证产品可制造性、工艺可操作性及合理性、设备精度及生产能力等,以保证零部件质量。本文针对柴油机新机型零部件开发中首件鉴定的应用进行探究,分析鉴定的步骤和流程,总结出首件鉴定过程中的质量控制要点,为后续其他类型产品开发的首件鉴定提供参考依据。 展开更多
关键词 首件鉴定 零部件开发 柴油机新机型 质量控制
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三维共价有机框架与分子筛复合材料的制备
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作者 马云超 韩雨航 +3 位作者 姚宇新 付跃 李京阳 刘春波 《吉林师范大学学报(自然科学版)》 2023年第3期103-107,共5页
将四(4-氨基苯基)甲烷与对苯二甲醛在溶剂热的条件下通过席夫碱反应合成具有亚胺键连接的三维共价有机框架材料(COF-300).选取常规水热法合成的分子筛SSZ-13,并用3-氨基丙基三乙基硅烷将分子筛表面进行后修饰氨基化,最后与COF-300进行... 将四(4-氨基苯基)甲烷与对苯二甲醛在溶剂热的条件下通过席夫碱反应合成具有亚胺键连接的三维共价有机框架材料(COF-300).选取常规水热法合成的分子筛SSZ-13,并用3-氨基丙基三乙基硅烷将分子筛表面进行后修饰氨基化,最后与COF-300进行复合得到COF-300@SSZ-13复合材料.粉末X射线衍射、扫描电子显微镜和元素分析仪等分析结果表明,COF-300和SSZ-13具有高的结晶性,COF-300在与分子筛复合后,均匀地生长在SSZ-13表面,证明了COF-300@SSZ-13复合材料的成功制备. 展开更多
关键词 三维共价有机框架 分子筛 复合材料
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