Background Since an effective method for generating induced pluripotent stem cells （iPSCs） from human neural stem cells （hNSCs） can offer us a promising tool for studying brain diseases, here we reported direct re...Background Since an effective method for generating induced pluripotent stem cells （iPSCs） from human neural stem cells （hNSCs） can offer us a promising tool for studying brain diseases, here we reported direct reprogramming of adult hNSCs into iPSCs by retroviral transduction of four defined factors. Methods NSCs were successfully isolated and cultured from the hippocampus tissue of epilepsy patients. When combined with four factors （OCT3/4, SOX2, KLF4, and c-MYC）, iPSCs colonies were successfully obtained. Results Morphological characterization and specific genetic expression confirmed that these hNSCs-derived iPSCs showed embryonic stem cells-like properties, which include the ability to differentiate into all three germ layers both in vitro and in vivo. Conclusion Our method would be useful for generating human iPSCs from NSCs and provide an important tool for studying neurological diseases.展开更多
基金This work was supported by grants from the Major State Basic Research Program （No. 2010CB945500, No. 2012CB966300, and No. 2009CB941100）, the National Natural Science Foundation of China （No. 81271003 and No. 81200936）, Shanghai Committee of Science and Technology （No. 08dj140053）, and 2011 Shanghai Medical College Young Scientist Fund of Fudan University （11L-24）.Acknowledgements： We are grateful to technicians CHEN Lu-ping, SHEN Yi-wen and TANG Qi-sheng in our lab for their kind assistance in animal preparation and cell culture. We also thank Dr. SHA Hong-ying for picture processing and helpful comments and suggestions.
文摘Background Since an effective method for generating induced pluripotent stem cells （iPSCs） from human neural stem cells （hNSCs） can offer us a promising tool for studying brain diseases, here we reported direct reprogramming of adult hNSCs into iPSCs by retroviral transduction of four defined factors. Methods NSCs were successfully isolated and cultured from the hippocampus tissue of epilepsy patients. When combined with four factors （OCT3/4, SOX2, KLF4, and c-MYC）, iPSCs colonies were successfully obtained. Results Morphological characterization and specific genetic expression confirmed that these hNSCs-derived iPSCs showed embryonic stem cells-like properties, which include the ability to differentiate into all three germ layers both in vitro and in vivo. Conclusion Our method would be useful for generating human iPSCs from NSCs and provide an important tool for studying neurological diseases.
