The aim of this study was to investigate the effects of polypeptides from Chlamys farreri （PCF） on immunoeytes or immunocytes treated with dexamethasone （DEX） in vitro. After incubating immunoeytes with 25 mg/L PC...The aim of this study was to investigate the effects of polypeptides from Chlamys farreri （PCF） on immunoeytes or immunocytes treated with dexamethasone （DEX） in vitro. After incubating immunoeytes with 25 mg/L PCF or/and DEX for a given time, the proliferative response of thymocytes and splenoeytes to ConA were measured bv MTF assay; the subpopulations of thymocytes and splenic T lymphoeytes was analyzed by flow cytomety; the cytotoxicity of natural killer cells was measured by Llactate dehydrogenase （LDH） assay; the phagocytosis of rat peritoneal macrophages was measured by Neutral red assay and the Bel-2 protein expression of macrophages was detected by imrnunocytoehemical stain. The proliferative ability of rat thymocytes and splenocytes induced with ConA was enhanced and the depression of lymphoproliferation caused by DEX was reversed by PCF. The percentages of mouse thymic L3 T4^- Lyt-2^- and Lyt-2^＋ subpopulations and splenic Lyt-2 ^＋ cells were decreased and the percentage of splenic L3 T4^ ＋ cells was increased by PCF. The NK cytotoxicity, phagocytosis of macraphages and Bcl-2 protein expression of macrophages were enhanced and the decrease of NK cytotoxicity and Bel-2 protein expression of maerophages caused by DEX were reversed by PCF. PCF could not only enhance the normal immunity function, but also reverse the imrnunosuppression induced by DEX.展开更多
文摘The aim of this study was to investigate the effects of polypeptides from Chlamys farreri （PCF） on immunoeytes or immunocytes treated with dexamethasone （DEX） in vitro. After incubating immunoeytes with 25 mg/L PCF or/and DEX for a given time, the proliferative response of thymocytes and splenoeytes to ConA were measured bv MTF assay; the subpopulations of thymocytes and splenic T lymphoeytes was analyzed by flow cytomety; the cytotoxicity of natural killer cells was measured by Llactate dehydrogenase （LDH） assay; the phagocytosis of rat peritoneal macrophages was measured by Neutral red assay and the Bel-2 protein expression of macrophages was detected by imrnunocytoehemical stain. The proliferative ability of rat thymocytes and splenocytes induced with ConA was enhanced and the depression of lymphoproliferation caused by DEX was reversed by PCF. The percentages of mouse thymic L3 T4^- Lyt-2^- and Lyt-2^＋ subpopulations and splenic Lyt-2 ^＋ cells were decreased and the percentage of splenic L3 T4^ ＋ cells was increased by PCF. The NK cytotoxicity, phagocytosis of macraphages and Bcl-2 protein expression of macrophages were enhanced and the decrease of NK cytotoxicity and Bel-2 protein expression of maerophages caused by DEX were reversed by PCF. PCF could not only enhance the normal immunity function, but also reverse the imrnunosuppression induced by DEX.
