Computational Analysis of Signal Peptide-Dependent Secreted Proteins in Saccharomyces cerevisiae

Computer based software such as the SignalP v3.0, TargetP v1.01, big-PI predictor and TMHMM v2.0 were combined to predict the signal peptides, and the signal peptide-dependent secreted proteins among the 6 700 ORFs in genome of Saccharomyces cerevisiae. The results showed that 163 proteins were the secreted ones containing signal peptides, and they were secreted via Sec pathway. Among the 163 predicted secreted proteins, the signal peptides of 47 secreted proteins included only the H-domain and C-domain, without N-domain, but the signal peptides of other 116 secreted proteins included all the three domains. There were differences in the constitution of signal peptides between the secreted proteins of S. cerevisiae and of Candida albicans, but the length and amino acids types of their signal peptides were similar in general. Few of the same signal peptides occurred in the secreted proteins of S. cerevisiae genome, and the homology could be compared among the secreted proteins with the same signal peptides. The BLAST 2 SEQUENECES and CLUSTAL W were used to align the two protein sequences and multi-protein sequences, respectively. The alignment result indicated that homology of these sequences with the same signal peptide was very highly conservative in amino acid of complete gene. The effect of the signal peptides in S. cerevisia on expression of foreign eukaryotic secreted proteins is discussed in this paper.

HPLC法同时测定老蔻丸中8个成分的含量

目的:建立HPLC法同时测定老蔻丸(陈皮、肉桂、丁香等)中8个成分的含量。方法:药物的甲醇提取液的采用依利特SinoChrom ODS-BP C_(18)色谱柱(200 mm×4.6 mm,5μm);流动相为乙腈-水-甲醇,梯度洗脱;流速为1.0 mL·min^(-1),柱温为30℃,检测波长为280 nm,进样量为15μL。结果:柚皮苷、橙皮苷、新橙皮苷、桂皮醛、丁香酚、和厚朴酚、厚朴酚、大黄酚质量浓度分别在26.669～400.031、26.803～402.042、13.335～200.028、2.566～38.484、4.272～64.086、19.994～299.908、26.682～400.223、1.068～16.014范围内与各自峰面积积分值呈良好的线性关系,相关系数r值均大于0.999;平均加样回收率(n=6)分别为100.7%、99.9%、100.1%、100.9%、99.9%、99.9%、100.6%、100.4%,RSD分别为0.77%、0.65%、0.55%、1.3%、1.4%、0.70%、0.56%、1.3%,3批样品中柚皮苷、橙皮苷、新橙皮苷、桂皮醛、丁香酚、和厚朴酚、厚朴酚、大黄酚的含量分别为1 487.42～1 678.33、6 203.50～6 982.31、1 272.29～1 410.19、236.49～292.03、1 869.33～1 896.70、3 767.62～4 073.41、2 879.84～3 250.23、279.55～301.75μg·g^(-1)。结论:该方法灵敏度高,专属性好,可用于老蔻丸的质量控制。

2015—2016年深圳市龙华区学校卫生常见病发病情况

目的了解2015—2016学年深圳市龙华区中小学生发育状况和常见病检测情况,为学校卫生工作提供依据。方法对2015—2016学年龙华区60所体检学校上报深圳市学生健康信息监测系统的身高、体重和常见病检出情况等数据进行分析。结果龙华区各学龄阶段学生身高上等率和下等率低于深圳市平均水平;各学龄组超重检出率为10.49%,低于深圳市平均水平11.80%;检出疾病以龋齿、视力不良、肥胖3种常见病为主,检出率依次为55.00%、8.31%、7.33%。其中龋齿(55.00%)和贫血的发病率(5.28%)较深圳市(40.03%、3.30%)高,且龋均最大值出现在小学组,恒龋失补(DMF)率均高于深圳市平均水平。视力不良发病率(38.31%)较深圳市低(46.98%),沙眼和脊柱侧弯发病率差异无统计学意义(P>0.05)。结论通过体检监测系统可以更全面,准确地分析龙华区学生健康状况,了解龙华区中小学生的生长发育情况和常见病的检出情况,有利于学校卫生工作顺利开展。

深圳市龙华区各级医疗机构医务人员手卫生现状调查

目的了解深圳市龙华区各级医疗机构医务人员手卫生依从性及卫生质量,以便加强医务人员手卫生管理。方法采用问卷调查和采样检测方法,对深圳市龙华区部分医疗机构医务人员手卫生现状进行调查。结果共调查深圳市龙华区38家不同级别医疗机构,采样132份,手卫生质量平均合格率为91.67%。公立医院、民营医院、社区健康中心和诊所医务人员手卫生指征知晓正确率分别为93.33%、86.00%、85.33%和81.00%,依从率分别为34.75%、40.00%、22.00%和31.25%。结论该区医疗机构的医务人员手卫生质量合格率91%以上,手卫生管理制度落实尚存在差距。