Objective To review the current advances on the role of uncoupling protein （UCP） in the pathogenesis and progress of nonalcoholic fatty liver disease （NAFLD）.Data sources A comprehensive search of the PubMed liter...Objective To review the current advances on the role of uncoupling protein （UCP） in the pathogenesis and progress of nonalcoholic fatty liver disease （NAFLD）.Data sources A comprehensive search of the PubMed literature without restriction on the publication date was carried out using keywords such as UCP and NAFLD.Study selection Articles containing information related to NAFLD and UCP were selected and carefully analyzed.Results The typical concepts,up-to-date findings,and existing controversies of UCP2 in NAFLD were summarized.Besides,the effect of a novel subtype of UCP （hepatocellular down regulated mitochondrial carrier protein,HDMCP） in NAFLD was also analyzed.Finally,the concept that any mitochondrial inner membrane carrier protein may have,more or less,the uncoupling ability was reinforced.Conclusions Considering the importance of NAFLD in clinics and UCP in energy metabolism,we believe that this review may raise research enthusiasm on the effect of UCP in NAFLD and provide a novel mechanism and therapeutic target for NAFLD.展开更多
Background It has been reported that CD8＋ regulatory cells could be induced upon oral tolerance. The purpose of this study was to investigate the changes of CD8a＋ T cells in dextran sulfate sodium （DSS）-induced co...Background It has been reported that CD8＋ regulatory cells could be induced upon oral tolerance. The purpose of this study was to investigate the changes of CD8a＋ T cells in dextran sulfate sodium （DSS）-induced colitis mice pretreated by oral immune regulation. Methods The effects of five low oral doses of colitis-extracted proteins （CEP） on colitis were evaluated by clinical manifestation and histological lesions. The percentages of CD8a＋ T cells gating on CD3＋ T cells were evaluated in the gut-associated lymphoid tissues （GALT） and the spleens by flow cytometry. Differences between the two groups were compared by Student＇s t test or Mann-Whitney U test. Results Compared to bovine serum albumin （BSA）-fed control mice, administration of CEP resulted in marked alleviation of colitis. The proportion of CDSa＋ T ceils, not only in intraepithelial lymphocytes （IELs） and lamina propria lymphocytes （LPLs） of the large intestine （LI） but also in spleen from CEP-fed colitis mice, was significantly higher than that from BSA-fed colitis mice （LI-IELs： （71.5±5.4）% vs. （60.1±4.3）%, P 〈0.01; LI-LPLs： （60.7±5.2）% vs. （51.9±4.7）%, P 〈0.01; spleen： （24.1±3.6）% vs. （20.3±4.1）%, P 〈0.05; n=8）. Mucosal repair in repair-period mice five days after termination of DSS treatment was also accompanied by an increase of CD8a＋ T cells in large intestinal mucosal lymphocytes （LI-IELs： （72.1±3.7）% vs. （61.5±4.5）%, P 〈0.01; LI-LPLs： （62.1±5.7）% vs. （52.7±3.6）%, P 〈0.01; n=8）. The proportion of CD3＋ T cells increased in Peyer＇s patches （PPs） and decreased in mesenteric lymph nodes （MLNs） from colitis mice compared to untreated mice, whereas the change pattern of CD3＋T cells in PPs and MLNs from CEP-fed colitis mice was just on the contrary. Conclusion Improvement of DSS-induced colitis resulted from oral immune regulation is associated with an increase in CD8a＋T cells in spleen and large intestinal mucosa.展开更多
基金This study was supported by grants from the National Natural Science Foundation of China （No. 81000169, No. 81100277, No. 81200284, No. 81230012）, the Excellent Young Investigator Foundation of Health Bureau of Zhejiang Province （No. 2010QNA011）, the Excellent Young Investigator Natural Science Foundation of Zhejiang province （No. R2110159）, and the Project of Zhejiang Traditional Chinese Medicine Administration Bureau （No. 2010ZA065）.
文摘Objective To review the current advances on the role of uncoupling protein （UCP） in the pathogenesis and progress of nonalcoholic fatty liver disease （NAFLD）.Data sources A comprehensive search of the PubMed literature without restriction on the publication date was carried out using keywords such as UCP and NAFLD.Study selection Articles containing information related to NAFLD and UCP were selected and carefully analyzed.Results The typical concepts,up-to-date findings,and existing controversies of UCP2 in NAFLD were summarized.Besides,the effect of a novel subtype of UCP （hepatocellular down regulated mitochondrial carrier protein,HDMCP） in NAFLD was also analyzed.Finally,the concept that any mitochondrial inner membrane carrier protein may have,more or less,the uncoupling ability was reinforced.Conclusions Considering the importance of NAFLD in clinics and UCP in energy metabolism,we believe that this review may raise research enthusiasm on the effect of UCP in NAFLD and provide a novel mechanism and therapeutic target for NAFLD.
基金This work was supported by a grant from the Natural Science Foundation of Zhejiang Province （No. Y2110864）. Conflicts of interest： None.
文摘Background It has been reported that CD8＋ regulatory cells could be induced upon oral tolerance. The purpose of this study was to investigate the changes of CD8a＋ T cells in dextran sulfate sodium （DSS）-induced colitis mice pretreated by oral immune regulation. Methods The effects of five low oral doses of colitis-extracted proteins （CEP） on colitis were evaluated by clinical manifestation and histological lesions. The percentages of CD8a＋ T cells gating on CD3＋ T cells were evaluated in the gut-associated lymphoid tissues （GALT） and the spleens by flow cytometry. Differences between the two groups were compared by Student＇s t test or Mann-Whitney U test. Results Compared to bovine serum albumin （BSA）-fed control mice, administration of CEP resulted in marked alleviation of colitis. The proportion of CDSa＋ T ceils, not only in intraepithelial lymphocytes （IELs） and lamina propria lymphocytes （LPLs） of the large intestine （LI） but also in spleen from CEP-fed colitis mice, was significantly higher than that from BSA-fed colitis mice （LI-IELs： （71.5±5.4）% vs. （60.1±4.3）%, P 〈0.01; LI-LPLs： （60.7±5.2）% vs. （51.9±4.7）%, P 〈0.01; spleen： （24.1±3.6）% vs. （20.3±4.1）%, P 〈0.05; n=8）. Mucosal repair in repair-period mice five days after termination of DSS treatment was also accompanied by an increase of CD8a＋ T cells in large intestinal mucosal lymphocytes （LI-IELs： （72.1±3.7）% vs. （61.5±4.5）%, P 〈0.01; LI-LPLs： （62.1±5.7）% vs. （52.7±3.6）%, P 〈0.01; n=8）. The proportion of CD3＋ T cells increased in Peyer＇s patches （PPs） and decreased in mesenteric lymph nodes （MLNs） from colitis mice compared to untreated mice, whereas the change pattern of CD3＋T cells in PPs and MLNs from CEP-fed colitis mice was just on the contrary. Conclusion Improvement of DSS-induced colitis resulted from oral immune regulation is associated with an increase in CD8a＋T cells in spleen and large intestinal mucosa.
