The N-terminal sequence of Luffaculin 1 was determined to be Asp-Val-Ser-Phe-Ser-. The CD spectrum of Luffaculin 1 indicated that Luffaculin 1 contains the 37.1% α-helix, 33.4% β-sheet,and 29.5% random coil. N-glyco...The N-terminal sequence of Luffaculin 1 was determined to be Asp-Val-Ser-Phe-Ser-. The CD spectrum of Luffaculin 1 indicated that Luffaculin 1 contains the 37.1% α-helix, 33.4% β-sheet,and 29.5% random coil. N-glycosidase activity of Luffaculin 1 against animal rRNA is observed. The anti-tumor activity of Luffaculin 1 on cell strains B16, MGC, Bel were determined,giving IC50 of 1.78×10^-7mol/L,2.11×10^-7mol/L,and 4.21×10^-7mol/L,respectively. We also discussed the N-terminal sequences, secondary structures,and bioactivities of Luffaculin 1 against Trichosanthin.展开更多
文摘The N-terminal sequence of Luffaculin 1 was determined to be Asp-Val-Ser-Phe-Ser-. The CD spectrum of Luffaculin 1 indicated that Luffaculin 1 contains the 37.1% α-helix, 33.4% β-sheet,and 29.5% random coil. N-glycosidase activity of Luffaculin 1 against animal rRNA is observed. The anti-tumor activity of Luffaculin 1 on cell strains B16, MGC, Bel were determined,giving IC50 of 1.78×10^-7mol/L,2.11×10^-7mol/L,and 4.21×10^-7mol/L,respectively. We also discussed the N-terminal sequences, secondary structures,and bioactivities of Luffaculin 1 against Trichosanthin.
