MPO在血管炎症中的研究进展

髓过氧化物酶(Myeloperoxidase,MPO)存在于髓系细胞中,是白细胞分泌的一种酶,催化形成多种活性氧化剂。MPO除参与防御反应外,还参与了氧化-炎症反应。血管炎症是指炎症细胞的激活并浸润血管壁导致血管壁损害的过程,临床上可表现为多种疾病。大量研究证实MPO通过氧化修饰参与血管炎症,在其疾病的发生、发展中发挥了重要的作用。MPO与动脉粥样硬化、糖尿病血管病变、过敏性紫癜、川崎病等存在密切的关系。文章将对MPO在血管炎症中的研究进展进行综述。

Determination of Quinolone Antibiotics in Water Using Solid Phase Extraction-High Performance Liquid Chromatography-Fluorescence Method

[Objective] To develop a solid phase extraction-high performance liquid chromatography-fluorescence method for determination of quin- olone antibiotics in water. [ Metbod] The standard curves of four quinolones （norfloxacin, ciprofloxacin, Iomefloxacin and enrofloxacin） were pre- pared. The detection limit in water and recovery were determined. The water samples collected from different areas, river and tap water were trea- ted using solid-phese extraction method and analyzed by high performance liquid chromatography. Then the concentration of quinolones antibiotics was determined by fluorescence method. [ Result] The detection limit of quinolone antibiotics in water was 0.083 -0.248 μg/L, and their recovery was 63.7% -134.1%. The four quinolone antibiotics at different levels were detected in various water samples, and the total concentration of quin- olone antibiotics was 0.045 -3.969 μg/L. The total concentration of quinolone antibiotics was higher in the water samples collected from rivers in Shenzhen area than in the sewage samples. The four quinolone antibiotics could be detected in all tap water samples. [ CoaduLsion ] The solid phase extraction-high performance liquid chromatography-fluorescence method is feasible and effective to detect quinolones in water. In addition, this method needs low cost and can meet requirements of daily monitorina and analysis.

血浆内皮素配合描记动态脑电图在儿童晕厥诊断中应用的临床意义

目的:探究血浆内皮素配合描记动态脑电图在儿童晕厥诊断中的应用意义。方法:选择2017年1月至2020年1月于我院接受治疗的83例存在晕厥风险儿童,采集其静脉血样进行血浆内皮素水平测定,并实施动态脑电图检测,而后以倾斜试验结果为金标准,分别分析单纯血浆内皮素、单纯动态脑电图以及血浆内皮素+动态脑电图对晕厥的诊断应用意义。结果:(1)83例入组儿童中阳性为68例,阴性为15例,血浆内皮素检测阳性51例,阴性32例,一致性为77.11%,灵敏度为73.53%,特异度为93.33%,阳性预测值为98.04%,阴性预测值为43.75%;(2)动态脑电图诊断一致性为78.31%,灵敏度为80.88%,特异度为66.67%,阳性预测值为91.67%,阴性预测值为43.48%;(3)联合检测诊断一致性为93.98%,灵敏度为94.12%,特异度为93.33%,阳性预测值为98.46%,阴性预测值为77.78%;(4)检测方式差异性比较发现,联合检测在一致性、灵敏度、阴性预测值方面明显优于血浆内皮素和动态脑电图检测,在特异度方面优于动态脑电图检测(P<0.05)。结论:血浆内皮素联合描记动态脑电图对儿童晕厥具有较好的诊断辅助价值,能够显著提高诊断的一致性、灵敏度和特异度。