A novel method was presented to create composite micelles of amphiphilic copolymers and Ag nanoparticles(NPs) in a three-dimensional co-flow focusing microfluidic device(3D CFMD). Self-assembly of the copolymers was i...A novel method was presented to create composite micelles of amphiphilic copolymers and Ag nanoparticles(NPs) in a three-dimensional co-flow focusing microfluidic device(3D CFMD). Self-assembly of the copolymers was initiated by the fast mixing of water and a blend dispersion of hydrophobic Ag NPs and amphiphilic copolymers. At the same time, the hydrophobic Ag NPs enter the core of copolymer micelles, based on the hydrophobic interaction. The copolymer-Ag NPs composite micelles have a core-shell structure with copolymer shell and Ag NPs core. COMSOL Multiphysics is used to simulate the concentration distribution of copolymers and Ag NPs under different flow rates. Co-assembly microfluidic conditions are determined based on simulation results. Under suitable microfluidic conditions, both block copolymers and gradient copolymers can co-assemble with hydrophobic Ag NPs to form composite micelles, respectively. This microfluidic coassembly method will have a good prospect in the preparation of composite micelles of amphiphilic copolymers and metal nanoparticles.展开更多
Abdominal aortic aneurysm(AAA) is a common and potentially dangerous vascular disease with many risk factors related to its occurrence and development. This review collects the results from recent studies of different...Abdominal aortic aneurysm(AAA) is a common and potentially dangerous vascular disease with many risk factors related to its occurrence and development. This review collects the results from recent studies of different comorbidities including hypertension, diabetes, and hyperlipidemia and summarizes their connections with AAA development and its underlying mechanisms. We believe that hypertension, diabetes, and hyperlipidemia can affect AAA occurrence and development, but more studies are needed to further explore the mechanisms.展开更多
To the Editor:Ruptured abdominal aortic aneurysm(rAAA)is a fatal disease.Emergency surgery is the only way to provide an opportunity for survival.In 1994,endovascular aortic repair(EVAR)was reported as an emergency tr...To the Editor:Ruptured abdominal aortic aneurysm(rAAA)is a fatal disease.Emergency surgery is the only way to provide an opportunity for survival.In 1994,endovascular aortic repair(EVAR)was reported as an emergency treatment for rAAA.Compared with traditional open surgery(OS),patients could benefit from its lower perioperative mortality and minimal invasiveness.Although recent guidelines have recommended an EVARfirst approach if suitable as judged by the clinician,the standard of whether to give a priority to EVAR varies among clinicians in practical applications,because hostile aortic morphology and hemodynamic conditions were thought to weaken the benefit of EVAR.Hence,controversies still exist regarding whether EVAR as a priority is applicable to most rAAAs.In addition,a search of the literature found few population-based studies with long-term outcomes.Given this,a population-based study was conducted by Zhongshan Hospital,Fudan University.展开更多
The efficacy of stem cell therapy is substantially compromised due to low cell survival rate and poor local retention post-delivery. These issues drastically limit the application of stem cells for ischemic limb thera...The efficacy of stem cell therapy is substantially compromised due to low cell survival rate and poor local retention post-delivery. These issues drastically limit the application of stem cells for ischemic limb therapy, which requires effective blood perfusion and skeletal muscle regeneration. Herein, based on microfluidic technology, an integrated stem cell and cytokine co-delivery system designed for functional ischemic limb salvage was constructed by first incorporating the myogenic cytokine, fibroblast growth factor 19 (FGF19), into microspheres composed of methacrylate gelatin (GelMA). Then adipose-derived stem cells (ADSCs) were highly absorbed into the porous structure of the microspheres, overcoming the insufficient loading efficiency and activities by conventional encapsulation strategy. The fabricated ADSCs/FGF19@μsphere system demonstrated a uniform size of about 180 μm and a highly porous structure with pore sizes between 20 and 40 μm. The resultant system allowed high doses of ADSCs to be precisely engrafted in the lesion and to survive, and achieved sustained FGF19 release in the ischemic region to facilitate myoblast recruitment and differentiation and myofibrils growth. Furthermore, the combination of ADSCs and FGF19 exhibited a positive synergistic effect which substantially improved the therapeutic benefit of angiogenesis and myogenesis, both in vitro and in vivo. In summary, a stem cell and cytokine co-delivery system with the properties of easy preparation and minimal invasiveness was designed to ensure highly efficient cell delivery, sustained cytokine release, and ultimately realizes effective treatment of ischemic limb regeneration.展开更多
It is known that the Proximal Extension To Induce Complete Attachment(PETTICOAT)technique improves the distal aortic remodeling compared with standard thoracic endovascular aortic repair(TEVAR).[1,2]However,the only a...It is known that the Proximal Extension To Induce Complete Attachment(PETTICOAT)technique improves the distal aortic remodeling compared with standard thoracic endovascular aortic repair(TEVAR).[1,2]However,the only available Zenith Dissection Endovascular System(Cook Medical Inc.,Bloomington,Ind,USA)has several limitations,including only two sizes(36 mm and 46 mm)and an one-taper design,which may not be suitable for the complex morphology of dissection at distal aorta,particular for the east Asian population.Thus,our center designed the new“Fabulous”stent system and acheieved better early curative effect.展开更多
Thoracic endovascular aortic repair(TEVAR)has been gradually considered as the mainstream therapy for type B aortic dissection(TBAD),especially for complicated acute TBAD,[1]due to its minimally invasive nature and sa...Thoracic endovascular aortic repair(TEVAR)has been gradually considered as the mainstream therapy for type B aortic dissection(TBAD),especially for complicated acute TBAD,[1]due to its minimally invasive nature and satisfactory clinical outcomes.Currently,our knowledge of aortic dissection and endovascular treatment is primarily derived from western registries including the International Registry of Acute Aortic Dissection(IRAD)and Vascular Quality Initiative(VQI),which includes few Asian TBAD patients without any Chinese population.Therefore,we report a long-term single-center experience of TBAD patients who underwent TEVAR in a Chinese high-volume tertiary center.The preliminary results would provide useful insight and enhances our understanding of this disease in the Chinese population.展开更多
基金Funded by the National Natural Science Foundation of China(Nos.51873167 and 50803048)
文摘A novel method was presented to create composite micelles of amphiphilic copolymers and Ag nanoparticles(NPs) in a three-dimensional co-flow focusing microfluidic device(3D CFMD). Self-assembly of the copolymers was initiated by the fast mixing of water and a blend dispersion of hydrophobic Ag NPs and amphiphilic copolymers. At the same time, the hydrophobic Ag NPs enter the core of copolymer micelles, based on the hydrophobic interaction. The copolymer-Ag NPs composite micelles have a core-shell structure with copolymer shell and Ag NPs core. COMSOL Multiphysics is used to simulate the concentration distribution of copolymers and Ag NPs under different flow rates. Co-assembly microfluidic conditions are determined based on simulation results. Under suitable microfluidic conditions, both block copolymers and gradient copolymers can co-assemble with hydrophobic Ag NPs to form composite micelles, respectively. This microfluidic coassembly method will have a good prospect in the preparation of composite micelles of amphiphilic copolymers and metal nanoparticles.
文摘Abdominal aortic aneurysm(AAA) is a common and potentially dangerous vascular disease with many risk factors related to its occurrence and development. This review collects the results from recent studies of different comorbidities including hypertension, diabetes, and hyperlipidemia and summarizes their connections with AAA development and its underlying mechanisms. We believe that hypertension, diabetes, and hyperlipidemia can affect AAA occurrence and development, but more studies are needed to further explore the mechanisms.
基金National Natural Science Foundation of China(No.8207021027)Shanghai Clinical Research Center for Interventional Medicine(No.19MC1910300)Shanghai Science and Technology Commission(Nos.19441906600 and 21S31904800)
文摘To the Editor:Ruptured abdominal aortic aneurysm(rAAA)is a fatal disease.Emergency surgery is the only way to provide an opportunity for survival.In 1994,endovascular aortic repair(EVAR)was reported as an emergency treatment for rAAA.Compared with traditional open surgery(OS),patients could benefit from its lower perioperative mortality and minimal invasiveness.Although recent guidelines have recommended an EVARfirst approach if suitable as judged by the clinician,the standard of whether to give a priority to EVAR varies among clinicians in practical applications,because hostile aortic morphology and hemodynamic conditions were thought to weaken the benefit of EVAR.Hence,controversies still exist regarding whether EVAR as a priority is applicable to most rAAAs.In addition,a search of the literature found few population-based studies with long-term outcomes.Given this,a population-based study was conducted by Zhongshan Hospital,Fudan University.
基金supported by the National Natural Science Foundation of China(grant number 8207021027)Shanghai Clinical Research Center for Interventional Medicine(grant number 19MC1910300)Shanghai Science and Technology Commission(grant numbers 19441906600,21S31904800).
文摘The efficacy of stem cell therapy is substantially compromised due to low cell survival rate and poor local retention post-delivery. These issues drastically limit the application of stem cells for ischemic limb therapy, which requires effective blood perfusion and skeletal muscle regeneration. Herein, based on microfluidic technology, an integrated stem cell and cytokine co-delivery system designed for functional ischemic limb salvage was constructed by first incorporating the myogenic cytokine, fibroblast growth factor 19 (FGF19), into microspheres composed of methacrylate gelatin (GelMA). Then adipose-derived stem cells (ADSCs) were highly absorbed into the porous structure of the microspheres, overcoming the insufficient loading efficiency and activities by conventional encapsulation strategy. The fabricated ADSCs/FGF19@μsphere system demonstrated a uniform size of about 180 μm and a highly porous structure with pore sizes between 20 and 40 μm. The resultant system allowed high doses of ADSCs to be precisely engrafted in the lesion and to survive, and achieved sustained FGF19 release in the ischemic region to facilitate myoblast recruitment and differentiation and myofibrils growth. Furthermore, the combination of ADSCs and FGF19 exhibited a positive synergistic effect which substantially improved the therapeutic benefit of angiogenesis and myogenesis, both in vitro and in vivo. In summary, a stem cell and cytokine co-delivery system with the properties of easy preparation and minimal invasiveness was designed to ensure highly efficient cell delivery, sustained cytokine release, and ultimately realizes effective treatment of ischemic limb regeneration.
基金National Science Foundation of China(No.81970412)Shanghai Municipal Science and Technology Commission Innovation Fund(No.22S31904800)+5 种基金Fujian Province Health Science and Technology Fund(No.2021GGB030)Shanghai Municipal Science and Technology Commission Innovation Fund(No.18441902400)National Clinical Research Center for Interventional Medicine Fund(No.2021-004)Shanghai Municipal Health Commission(No.20214Y0474)Xiamen Municipal Health Science And Technology Program Fund(No.3502Z20194034)Fudan Zhangjiang Clinical Medicine Innovation Fund(No.KP7202115)
文摘It is known that the Proximal Extension To Induce Complete Attachment(PETTICOAT)technique improves the distal aortic remodeling compared with standard thoracic endovascular aortic repair(TEVAR).[1,2]However,the only available Zenith Dissection Endovascular System(Cook Medical Inc.,Bloomington,Ind,USA)has several limitations,including only two sizes(36 mm and 46 mm)and an one-taper design,which may not be suitable for the complex morphology of dissection at distal aorta,particular for the east Asian population.Thus,our center designed the new“Fabulous”stent system and acheieved better early curative effect.
基金a grant from the National Natural Science Foundation of China(No.82070497).
文摘Thoracic endovascular aortic repair(TEVAR)has been gradually considered as the mainstream therapy for type B aortic dissection(TBAD),especially for complicated acute TBAD,[1]due to its minimally invasive nature and satisfactory clinical outcomes.Currently,our knowledge of aortic dissection and endovascular treatment is primarily derived from western registries including the International Registry of Acute Aortic Dissection(IRAD)and Vascular Quality Initiative(VQI),which includes few Asian TBAD patients without any Chinese population.Therefore,we report a long-term single-center experience of TBAD patients who underwent TEVAR in a Chinese high-volume tertiary center.The preliminary results would provide useful insight and enhances our understanding of this disease in the Chinese population.
