The main methods of coalbed methane(CBM)development are drainage and depressurization,and a precise prediction of coal reservoir pressure is thus crucial for the evaluation of reservoir potentials and the formulation ...The main methods of coalbed methane(CBM)development are drainage and depressurization,and a precise prediction of coal reservoir pressure is thus crucial for the evaluation of reservoir potentials and the formulation of reasonable development plans.This work established a new reservoir pressure prediction model based on the material balance equation(MBE)of coal reservoir,which considers the self-regulating effects of coal reservoirs and the dynamic change of equivalent drainage area(EDA).According to the proposed model,the reservoir pressure can be predicted based on reservoir condition data and the actual production data of a single well.Compared with traditional reservoir pressure prediction models which regard EDA as a fixed value,the proposed model can better predict the average pressure of reservoirs.Moreover,orthogonal experiments were designed to evaluate the sensitivity of reservoir parameters on the reservoir pressure prediction results of this proposed model.The results show that the saturation of irreducible water is the most sensitive parameter,followed by Langmuir volume and reservoir porosity,and Langmuir pressure is the least sensitive parameter.In addition,the pressure drop of reservoirs is negatively correlated with the saturation of irreducible water and the Langmuir volume,while it is positively correlated with porosity.This work analyzed the reservoir pressure drop characteristics of the CBM wells in the Shizhuangnan Block of the Qinshui Basin,and the results show that the CBM reservoir depressurization can be divided into three types,i.e.,rapidly drop type,medium-term stability type,and slowly drop type.The drainage features of wells were reasonably interpreted based on the comprehensive analysis of the reservoir depressurization type;the latter was coupled to the corresponding permeability dynamic change characteristics,eventually proving the applicability of the proposed model.展开更多
目的采用脂多糖(LPS)处理膀胱癌(BC)5637细胞以模拟炎症微环境,研究炎症对BC细胞侵袭迁移的影响及由NOD样受体热蛋白结构域相关蛋白3(NLRP3)介导的调控机制。方法将5637细胞分为LPS组、MCC950组、LPS/MCC950组和空白对照组4组,采用CCK-...目的采用脂多糖(LPS)处理膀胱癌(BC)5637细胞以模拟炎症微环境,研究炎症对BC细胞侵袭迁移的影响及由NOD样受体热蛋白结构域相关蛋白3(NLRP3)介导的调控机制。方法将5637细胞分为LPS组、MCC950组、LPS/MCC950组和空白对照组4组,采用CCK-8实验检测细胞增殖活力,Transwell侵袭实验检测细胞侵袭能力,细胞划痕实验检测细胞迁移能力,qRT-PCR检测基质金属蛋白酶-2(MMP-2)、钙黏蛋白E(E-cadhjerin)、波形蛋白(Vimentin)、NLRP3 m RNA表达水平,Western blot检测MMP-2、基质金属蛋白酶-9(MMP-9)、E-cadherin、Vimentin、NLRP3蛋白表达水平。结果LPS可显著增强5637细胞的增殖活力,促进其侵袭迁移及上皮间充质转化,并上调NLRP3 mRNA和蛋白的表达;MCC950可显著下调NLRP3蛋白的表达,并抑制LPS促进5637细胞侵袭迁移的效应。结论LPS可通过诱导NLRP3表达来促进BC细胞侵袭迁移,NLRP3炎症小体可能是炎症状态下促进BC进展的重要因子。展开更多
目的分析终末期肝病肝移植受者围手术期的诸多指标,寻找影响短期预后(≤3个月)的相关因素。方法回顾性分析上海海军军医大学附属东方肝胆外科医院单一治疗中心165例终末期肝病接受肝移植治疗的终末期肝病受者资料。将受者性别、年龄、...目的分析终末期肝病肝移植受者围手术期的诸多指标,寻找影响短期预后(≤3个月)的相关因素。方法回顾性分析上海海军军医大学附属东方肝胆外科医院单一治疗中心165例终末期肝病接受肝移植治疗的终末期肝病受者资料。将受者性别、年龄、体重指数、术前血清白蛋白水平、血清钠浓度、尿素氮水平及供受者血型等围手术期指标运用Logistic回归分析进行处理。结果165例受者术后3个月内死亡18例,死亡率为10.9%。单因素分析提示术前血清钠、血尿素氮、凝血酶原时间国际标准化比值(PT-INR)、Child-Turcotte-Pugh评分(CTP评分)、终末期肝病模型(Model for End-Stage Liver Disease,MELD)评分和终末期肝病模型-血清钠(MELD-Na)积分与预后的相关性具有统计学意义;而多元Logistic逐步回归分析提示只有术前MELD-Na积分具有统计学意义[P=0.001,β=-2.510,优势比=0.088,95%置信区间为(0.037,0.349)]。结论术前MELD-Na积分是终末期肝病受者肝移植术后短期存活的独立危险因素,积分越高短期死亡风险越大。展开更多
本研究拟探讨致炎因子脂多糖(lipopolysaccharide,LPS)对人膀胱癌5637细胞增殖及NOD样受体热蛋白结构域相关蛋白3(NOD-like receptor thermal protein domain associated protein 3,NLRP3)表达的影响。一、材料与方法1.材料:人膀胱癌563...本研究拟探讨致炎因子脂多糖(lipopolysaccharide,LPS)对人膀胱癌5637细胞增殖及NOD样受体热蛋白结构域相关蛋白3(NOD-like receptor thermal protein domain associated protein 3,NLRP3)表达的影响。一、材料与方法1.材料:人膀胱癌5637细胞、LPS、NLRP3抗体、NLRP3特异性抑制剂MCC950、细胞计数试剂盒(cell counting kit-8,CCK-8)、细胞周期试剂盒。展开更多
基金financially supported by the National Natural Science Foundation of China (grants No. 41772159/D0208, No. 41872178)the National Science and Technology Major Project of China (grant No. 2017ZX05064003)
文摘The main methods of coalbed methane(CBM)development are drainage and depressurization,and a precise prediction of coal reservoir pressure is thus crucial for the evaluation of reservoir potentials and the formulation of reasonable development plans.This work established a new reservoir pressure prediction model based on the material balance equation(MBE)of coal reservoir,which considers the self-regulating effects of coal reservoirs and the dynamic change of equivalent drainage area(EDA).According to the proposed model,the reservoir pressure can be predicted based on reservoir condition data and the actual production data of a single well.Compared with traditional reservoir pressure prediction models which regard EDA as a fixed value,the proposed model can better predict the average pressure of reservoirs.Moreover,orthogonal experiments were designed to evaluate the sensitivity of reservoir parameters on the reservoir pressure prediction results of this proposed model.The results show that the saturation of irreducible water is the most sensitive parameter,followed by Langmuir volume and reservoir porosity,and Langmuir pressure is the least sensitive parameter.In addition,the pressure drop of reservoirs is negatively correlated with the saturation of irreducible water and the Langmuir volume,while it is positively correlated with porosity.This work analyzed the reservoir pressure drop characteristics of the CBM wells in the Shizhuangnan Block of the Qinshui Basin,and the results show that the CBM reservoir depressurization can be divided into three types,i.e.,rapidly drop type,medium-term stability type,and slowly drop type.The drainage features of wells were reasonably interpreted based on the comprehensive analysis of the reservoir depressurization type;the latter was coupled to the corresponding permeability dynamic change characteristics,eventually proving the applicability of the proposed model.
文摘目的采用脂多糖(LPS)处理膀胱癌(BC)5637细胞以模拟炎症微环境,研究炎症对BC细胞侵袭迁移的影响及由NOD样受体热蛋白结构域相关蛋白3(NLRP3)介导的调控机制。方法将5637细胞分为LPS组、MCC950组、LPS/MCC950组和空白对照组4组,采用CCK-8实验检测细胞增殖活力,Transwell侵袭实验检测细胞侵袭能力,细胞划痕实验检测细胞迁移能力,qRT-PCR检测基质金属蛋白酶-2(MMP-2)、钙黏蛋白E(E-cadhjerin)、波形蛋白(Vimentin)、NLRP3 m RNA表达水平,Western blot检测MMP-2、基质金属蛋白酶-9(MMP-9)、E-cadherin、Vimentin、NLRP3蛋白表达水平。结果LPS可显著增强5637细胞的增殖活力,促进其侵袭迁移及上皮间充质转化,并上调NLRP3 mRNA和蛋白的表达;MCC950可显著下调NLRP3蛋白的表达,并抑制LPS促进5637细胞侵袭迁移的效应。结论LPS可通过诱导NLRP3表达来促进BC细胞侵袭迁移,NLRP3炎症小体可能是炎症状态下促进BC进展的重要因子。
文摘目的分析终末期肝病肝移植受者围手术期的诸多指标,寻找影响短期预后(≤3个月)的相关因素。方法回顾性分析上海海军军医大学附属东方肝胆外科医院单一治疗中心165例终末期肝病接受肝移植治疗的终末期肝病受者资料。将受者性别、年龄、体重指数、术前血清白蛋白水平、血清钠浓度、尿素氮水平及供受者血型等围手术期指标运用Logistic回归分析进行处理。结果165例受者术后3个月内死亡18例,死亡率为10.9%。单因素分析提示术前血清钠、血尿素氮、凝血酶原时间国际标准化比值(PT-INR)、Child-Turcotte-Pugh评分(CTP评分)、终末期肝病模型(Model for End-Stage Liver Disease,MELD)评分和终末期肝病模型-血清钠(MELD-Na)积分与预后的相关性具有统计学意义;而多元Logistic逐步回归分析提示只有术前MELD-Na积分具有统计学意义[P=0.001,β=-2.510,优势比=0.088,95%置信区间为(0.037,0.349)]。结论术前MELD-Na积分是终末期肝病受者肝移植术后短期存活的独立危险因素,积分越高短期死亡风险越大。
文摘本研究拟探讨致炎因子脂多糖(lipopolysaccharide,LPS)对人膀胱癌5637细胞增殖及NOD样受体热蛋白结构域相关蛋白3(NOD-like receptor thermal protein domain associated protein 3,NLRP3)表达的影响。一、材料与方法1.材料:人膀胱癌5637细胞、LPS、NLRP3抗体、NLRP3特异性抑制剂MCC950、细胞计数试剂盒(cell counting kit-8,CCK-8)、细胞周期试剂盒。
