Scrub typhus is an acute febrile vector-borne zoonotic disease caused by the obligate intracellular growth bacterium Orientia tsutsugamushi(Ot).Mites are the primary vectors and rodents play a pivotal role in the tran...Scrub typhus is an acute febrile vector-borne zoonotic disease caused by the obligate intracellular growth bacterium Orientia tsutsugamushi(Ot).Mites are the primary vectors and rodents play a pivotal role in the transmission of scrub typhus.Due to the climate warming,increased human activity and other factors,cases of scrub typhus have been increased sharply during the past decade in China,especially in the northern China.To understand the incidence trend,epidemic pattern,clinical sign,diagnosis and therapy of this diseases as well as genotype evolution of Orientia tsutsugamushi,we summarized and analyzed the current knowledge of scrub typhus in China from 2010 to 2020.The data indicated that the dominate genotypes of scrub typhus in China were Karp,Kato and Gilliam.Although the disease was distributed national wide,Yunnan,Guangzhou and Fujian showed the highest incidence rate.The main vector of scrub typhus in southwest,middle east and southeast of China appeared a geographic preference respectively.Seasonal timing,age and occupation were the key factors that relate to the peak incidence of scrub typhus.Notably,farmer was the occupation with the highest risk of Ot infection.Further study on the epidemic characteristics,risk factors,diagnosis and treatments of scrub typhus will be of benefit to a comprehensive guideline for prevention and control of this ancient disease.展开更多
Objective:To evaluate the legacy and novel per-and polyfluoroalkyl substances(PFAS)exposure in women of reproductive age,and to explore the effects on female reproductive hormones.Methods:We used cross-sectional data ...Objective:To evaluate the legacy and novel per-and polyfluoroalkyl substances(PFAS)exposure in women of reproductive age,and to explore the effects on female reproductive hormones.Methods:We used cross-sectional data of 433 normal women of reproductive age from 2013 to 2015.Data of age,age at menarche,parity,BMI,education,and income were collected through questionnaires,serum samples were collected for reproductive hormones,and plasma samples for PFAS measurement by ultraperformance liquid chromatography-tandem mass spectrometer(UPLC-MS/MS).Multiple linear regression and quantile g-computation were adopted to analyze the association between PFAS and reproductive hormones.Results:The detection rates of 6:2 Cl-PFESA,8:2 Cl-PFESA and HFPO-DA in the plasma were 100%,99.8%and 99.9%,respectively.The median concentrations of these chemicals were 2.27 ng/mL,0.07 ng/mL and 0.03 ng/mL,respectively.Multiple linear regression analysis showed a positive association between branched PFOS[1m-PFOS(β=0.131,95%CI:0.021,0.242),br-PFOS(β=0.119,95%CI:0.005,0.234)]and progesterone.In addition,mixed exposure model suggested that PFAS had a positive association with progesterone(β=0.549,95%CI:0.323,0.774).Conclusion:In this study,a mixed exposure model was used to evaluate the combined effects of PFAS mixtures,reflecting the association between multiple environmental PFAS exposure and reproductive hormones,and a higher concentration of novel PFAS was found in women of reproductive age,confirming that PFAS exposure may be related to reproductive hormone disorders in women of reproductive age.展开更多
Arenaviruses belong to the family of RNA viruses that can infect humans in various ways and cause different degrees of mortality.Rodents is the mainly hosts.Human pathogenic arenaviruses include lymphocytic choroid me...Arenaviruses belong to the family of RNA viruses that can infect humans in various ways and cause different degrees of mortality.Rodents is the mainly hosts.Human pathogenic arenaviruses include lymphocytic choroid meningitis,Lassa virus group and Takarib virus group,which cause human lymphocytic choriomeningitis and human hemorrhagic diseases.Rodents account for about 43%of mammalian species.At present,more than 30 highly pathogenic viruses have been found in rodents,including arenaviruses.The arenaviridae that infects rodents are mainly mammalian arenaviruses.This article reviews the etiology,clinicopathology,epidemiology,prevention and control of arenavirus,and provides references for the research and prevention of arenavirus.展开更多
The total fertility rate of women in childbearing age showed a downward trend in China.In addition to the age and genetic factors,environmental endocrine disruption can also impair fertility.The impact of increasing n...The total fertility rate of women in childbearing age showed a downward trend in China.In addition to the age and genetic factors,environmental endocrine disruption can also impair fertility.The impact of increasing new environmental pollutants on the couples in childbearing age has become a research hotspot recently.Phthalate acid esters(PAEs)is a common plasticizer in plastic products,which is widely found in toys,food packaging,construction materials,electronic and medical components,personal care products,office and school supplies and other plastic packaging products,and is the main substance of environmental pollution.Multiple studies have shown that PAEs can not only cause environmental and water pollution,but also have a variety of toxic effects such as reproductive toxicity,genotoxicity,immunotoxicity,neurotoxicity,teratogenicity,and carcinogenesis.Therefore,its impact on human health,especially on reproductive health of people of reproductive age and their offspring,cannot be ignored.However,the current epidemiological study of PAEs and new alternatives in reproductive health population is still controversial,and the toxicity mechanism is still in the exploration stage.This article through to PAEs of parental generation,children(including embryo)of reproductive development and the influence of genetic toxicity research progress at home and abroad to do a review,aims to promote effective control measures for the establishment of PAEs pollutants rather than on reproductive health risk prediction,thus for PAEs of adverse reproductive outcomes of reproductive stage of people provide a scientific basis for precision control and guidance.展开更多
为建立可以同时检测犬瘟热病毒(CDV)和犬副流感病毒(CPIV)的检测方法,依据GenBank中登录的CDV的H基因和CPIV的NP基因保守序列,设计合成扩增序列分别为593 bp (CDV H基因)、1 530 bp (CPIV NP基因)的2对引物,通过优化反应条件,建立一种...为建立可以同时检测犬瘟热病毒(CDV)和犬副流感病毒(CPIV)的检测方法,依据GenBank中登录的CDV的H基因和CPIV的NP基因保守序列,设计合成扩增序列分别为593 bp (CDV H基因)、1 530 bp (CPIV NP基因)的2对引物,通过优化反应条件,建立一种能够同时检测CDV和CPIV的双重一步法RT-PCR检测方法。特异性和敏感性试验显示,该方法能特异地检测CDV和CPIV,其最低检测限分别为3.58×10~6拷贝/μL和1.74×10~6拷贝/μL。对51份临床疑似病料样品进行检测,同时分别采用商品化的CDV和CPIV抗原检测试剂盒进行检测,结果二者符合率为100%。该结果表明本实验所建立的双重一步法RT-PCR方法的灵敏度及特异性均较好,可用于临床相关疫病的诊断。展开更多
Objective:To study the in vitro virucidal activity of 9 extracts of traditional Chinese herbal medicine(the water extracts of Evodia lepta,Clausena lansium,Clerodendrum cyrtophyllum,Callicarpa nudiflora,Nauclea offici...Objective:To study the in vitro virucidal activity of 9 extracts of traditional Chinese herbal medicine(the water extracts of Evodia lepta,Clausena lansium,Clerodendrum cyrtophyllum,Callicarpa nudiflora,Nauclea officinalis and Elaeagnus gonyanthes,the alcohol extracts of Nauclea officinalis,Elaeagnus gonyanthes and Zanthoxylumarmatum)on human respiratory syncytial virus(HRSV).Methods:The cytotoxic effect of the extracts on cells was evaluated by a cell viability assay using the CCK-8 method,a concentration of the extracts with cell viability greater than 50%was selected for the follow-up anti-HRSV effect assay,the 50%effective concentration(EC50)was assessed by an in vitro cell infection model.Results:The EC50s of the water extract from Clerodendrum cyrtophyllum,Callicarpa nudiflora and Elaeagnus gonyanthes were 0.05 mg/mL,0.03 mg/mL and 0.05 mg/mL,and the therapeutic index(TI)of them were 18.60,21.67 and 56.80 respectively.Conclusion:The water extracts of Clerodendrum cyrtophyllum,Callicarpa nudiflora and Elaeagnus gonyanthes possess the activity of anti-HRSV virus.展开更多
目的了解可能影响急诊科护士灾害准备度的因素。方法检索PubMed、Web of Science、EMbase、CINAHL、中国知网、万方及维普数据库,检索时间为建库至2022年8月,纳入有关我国急诊科护士灾害准备度影响因素的相关文献,进行筛选、提取、评价...目的了解可能影响急诊科护士灾害准备度的因素。方法检索PubMed、Web of Science、EMbase、CINAHL、中国知网、万方及维普数据库,检索时间为建库至2022年8月,纳入有关我国急诊科护士灾害准备度影响因素的相关文献,进行筛选、提取、评价文献,使用RevMan 5.3软件进行Meta分析。结果共纳入7篇文献,总样本量为2418例;Meta分析显示,灾害课程培训、救援经验、职称及学历是影响急诊科护士灾害准备度的因素。结论灾害课程培训、救援经验、职称以及学历是我国急诊科护士灾害准备度的影响因素,护理管理者应制定相应改进政策提升急诊科护士的备灾能力。展开更多
基金High-level Talent Project of the Hainan Province (No.820RC628)National Natural Science Foundation of China (No.82060378,81860367)+1 种基金Natural Science Foundation Youth Project of Hainan Province (No.820QN267)Cultivation Fund of Hainan Medical College (No.HYPY201919)。
文摘Scrub typhus is an acute febrile vector-borne zoonotic disease caused by the obligate intracellular growth bacterium Orientia tsutsugamushi(Ot).Mites are the primary vectors and rodents play a pivotal role in the transmission of scrub typhus.Due to the climate warming,increased human activity and other factors,cases of scrub typhus have been increased sharply during the past decade in China,especially in the northern China.To understand the incidence trend,epidemic pattern,clinical sign,diagnosis and therapy of this diseases as well as genotype evolution of Orientia tsutsugamushi,we summarized and analyzed the current knowledge of scrub typhus in China from 2010 to 2020.The data indicated that the dominate genotypes of scrub typhus in China were Karp,Kato and Gilliam.Although the disease was distributed national wide,Yunnan,Guangzhou and Fujian showed the highest incidence rate.The main vector of scrub typhus in southwest,middle east and southeast of China appeared a geographic preference respectively.Seasonal timing,age and occupation were the key factors that relate to the peak incidence of scrub typhus.Notably,farmer was the occupation with the highest risk of Ot infection.Further study on the epidemic characteristics,risk factors,diagnosis and treatments of scrub typhus will be of benefit to a comprehensive guideline for prevention and control of this ancient disease.
基金Hainan Clinical Medical Center Construction Project(Qiongwei Yihan[2021]No.75)。
文摘Objective:To evaluate the legacy and novel per-and polyfluoroalkyl substances(PFAS)exposure in women of reproductive age,and to explore the effects on female reproductive hormones.Methods:We used cross-sectional data of 433 normal women of reproductive age from 2013 to 2015.Data of age,age at menarche,parity,BMI,education,and income were collected through questionnaires,serum samples were collected for reproductive hormones,and plasma samples for PFAS measurement by ultraperformance liquid chromatography-tandem mass spectrometer(UPLC-MS/MS).Multiple linear regression and quantile g-computation were adopted to analyze the association between PFAS and reproductive hormones.Results:The detection rates of 6:2 Cl-PFESA,8:2 Cl-PFESA and HFPO-DA in the plasma were 100%,99.8%and 99.9%,respectively.The median concentrations of these chemicals were 2.27 ng/mL,0.07 ng/mL and 0.03 ng/mL,respectively.Multiple linear regression analysis showed a positive association between branched PFOS[1m-PFOS(β=0.131,95%CI:0.021,0.242),br-PFOS(β=0.119,95%CI:0.005,0.234)]and progesterone.In addition,mixed exposure model suggested that PFAS had a positive association with progesterone(β=0.549,95%CI:0.323,0.774).Conclusion:In this study,a mixed exposure model was used to evaluate the combined effects of PFAS mixtures,reflecting the association between multiple environmental PFAS exposure and reproductive hormones,and a higher concentration of novel PFAS was found in women of reproductive age,confirming that PFAS exposure may be related to reproductive hormone disorders in women of reproductive age.
基金Hainan Provincial Higher Education Science Research Project(Hnky 2020‑33)Key Research and Development Plan of Hainan Province(ZDYF2020150)+4 种基金Major Science and Technology Project of Hainan Province(ZDKJ202003)National Natural Science Foundation of China(32060015)High level Talent Program of Hainan Natural Science Foundation(2019RC218)Hainan Academician Innovation Platform Project(YSPTZX202004)Hainan Academician Workstation Project(SRC200003)。
文摘Arenaviruses belong to the family of RNA viruses that can infect humans in various ways and cause different degrees of mortality.Rodents is the mainly hosts.Human pathogenic arenaviruses include lymphocytic choroid meningitis,Lassa virus group and Takarib virus group,which cause human lymphocytic choriomeningitis and human hemorrhagic diseases.Rodents account for about 43%of mammalian species.At present,more than 30 highly pathogenic viruses have been found in rodents,including arenaviruses.The arenaviridae that infects rodents are mainly mammalian arenaviruses.This article reviews the etiology,clinicopathology,epidemiology,prevention and control of arenavirus,and provides references for the research and prevention of arenavirus.
基金Hainan Province Clinical Medical Center (No.QWYH202175).
文摘The total fertility rate of women in childbearing age showed a downward trend in China.In addition to the age and genetic factors,environmental endocrine disruption can also impair fertility.The impact of increasing new environmental pollutants on the couples in childbearing age has become a research hotspot recently.Phthalate acid esters(PAEs)is a common plasticizer in plastic products,which is widely found in toys,food packaging,construction materials,electronic and medical components,personal care products,office and school supplies and other plastic packaging products,and is the main substance of environmental pollution.Multiple studies have shown that PAEs can not only cause environmental and water pollution,but also have a variety of toxic effects such as reproductive toxicity,genotoxicity,immunotoxicity,neurotoxicity,teratogenicity,and carcinogenesis.Therefore,its impact on human health,especially on reproductive health of people of reproductive age and their offspring,cannot be ignored.However,the current epidemiological study of PAEs and new alternatives in reproductive health population is still controversial,and the toxicity mechanism is still in the exploration stage.This article through to PAEs of parental generation,children(including embryo)of reproductive development and the influence of genetic toxicity research progress at home and abroad to do a review,aims to promote effective control measures for the establishment of PAEs pollutants rather than on reproductive health risk prediction,thus for PAEs of adverse reproductive outcomes of reproductive stage of people provide a scientific basis for precision control and guidance.
文摘为建立可以同时检测犬瘟热病毒(CDV)和犬副流感病毒(CPIV)的检测方法,依据GenBank中登录的CDV的H基因和CPIV的NP基因保守序列,设计合成扩增序列分别为593 bp (CDV H基因)、1 530 bp (CPIV NP基因)的2对引物,通过优化反应条件,建立一种能够同时检测CDV和CPIV的双重一步法RT-PCR检测方法。特异性和敏感性试验显示,该方法能特异地检测CDV和CPIV,其最低检测限分别为3.58×10~6拷贝/μL和1.74×10~6拷贝/μL。对51份临床疑似病料样品进行检测,同时分别采用商品化的CDV和CPIV抗原检测试剂盒进行检测,结果二者符合率为100%。该结果表明本实验所建立的双重一步法RT-PCR方法的灵敏度及特异性均较好,可用于临床相关疫病的诊断。
基金Key Science and Technology Project of Hainan Province(No.ZDKJ202003)Hainan Provincial Natural Science Foundation of China(No.821QN262)+1 种基金Hainan Provincial Natural Science Foundation of China(No.2019RC207)Hainan Provincial Natural Science Foundation of China(No.820RC628)。
文摘Objective:To study the in vitro virucidal activity of 9 extracts of traditional Chinese herbal medicine(the water extracts of Evodia lepta,Clausena lansium,Clerodendrum cyrtophyllum,Callicarpa nudiflora,Nauclea officinalis and Elaeagnus gonyanthes,the alcohol extracts of Nauclea officinalis,Elaeagnus gonyanthes and Zanthoxylumarmatum)on human respiratory syncytial virus(HRSV).Methods:The cytotoxic effect of the extracts on cells was evaluated by a cell viability assay using the CCK-8 method,a concentration of the extracts with cell viability greater than 50%was selected for the follow-up anti-HRSV effect assay,the 50%effective concentration(EC50)was assessed by an in vitro cell infection model.Results:The EC50s of the water extract from Clerodendrum cyrtophyllum,Callicarpa nudiflora and Elaeagnus gonyanthes were 0.05 mg/mL,0.03 mg/mL and 0.05 mg/mL,and the therapeutic index(TI)of them were 18.60,21.67 and 56.80 respectively.Conclusion:The water extracts of Clerodendrum cyrtophyllum,Callicarpa nudiflora and Elaeagnus gonyanthes possess the activity of anti-HRSV virus.
文摘目的了解可能影响急诊科护士灾害准备度的因素。方法检索PubMed、Web of Science、EMbase、CINAHL、中国知网、万方及维普数据库,检索时间为建库至2022年8月,纳入有关我国急诊科护士灾害准备度影响因素的相关文献,进行筛选、提取、评价文献,使用RevMan 5.3软件进行Meta分析。结果共纳入7篇文献,总样本量为2418例;Meta分析显示,灾害课程培训、救援经验、职称及学历是影响急诊科护士灾害准备度的因素。结论灾害课程培训、救援经验、职称以及学历是我国急诊科护士灾害准备度的影响因素,护理管理者应制定相应改进政策提升急诊科护士的备灾能力。
